MBD1 polyclonal antibody

50 μg /84 μl
  Bulk order

Alternative names: CXXC3, PCM1, RFT

Polyclonal antibody raised in rabbit against human MBD1 (Methyl-CpG-binding domain protein 1), using 3 KLH-conjugated synthetic peptides, two containing a sequence from the C-terminal part and one containing a sequence from the N-terminal part of the protein. 

Concentration0.6 µg/µl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:1,000 Fig 1
Western Blotting 1:1,000 Fig 2
  • Validation Data


    Figure 1. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD1 (cat. No. C15410038) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against MBD1
    HeLa cells were transfected with an expression vector for TY1-tagged MBD1. The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against MBD1 (cat. No. C15410038) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.

  •  应用
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  文档
    Datasheet MBD1 pAb-038-050 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Safety sheets
    MBD1 Antibody SDS GB en Download
    MBD1 Antibody SDS US en Download
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    MBD1 Antibody SDS FR fr Download
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: MBD1 polyclonal antibody (Diagenode Cat# C15410038 Lot# A1223P). Click here to copy to clipboard.

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