Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K36me3
ChIP was performed with the Diagenode antibody against H3K36me3 (cat. No. C15210013) on sheared chromatin from 500,000 HeLaS3 cells using the “iDeal ChIP-seq” kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the CCT5 coding region, used as positive control, and for a region upstream the ACTB promoter, used as negative control. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).