Diagenode

Flp polyclonal antibody (Sample Size)

目录号
格式
价格
C15310169-20
20 µl
$100.00
  Bulk order
其他格式



Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein.

LotA280-004
Concentrationnot determined
Species reactivity
TypePolyclonal
PurityWhole antiserum from rabbit containing 0.05% azide.
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:500 Fig 1
Western Blotting 1:500 Fig 2, (1)

  • Validation data

    ELISA

    Figure 1. Determination of the antibody titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. CS-169-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against Flp
    Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. CS-169-100), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.

  •  应用
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ELISA
    Enzyme-linked immunosorbent assay. Read more
  •  文档
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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    Datasheet Flp C15310169 DATASHEET
    Datasheet description
    Download
  •  Safety sheets
    Flp polyclonal antibody SDS GB en Download
    Flp polyclonal antibody SDS US en Download
    Flp polyclonal antibody SDS DE de Download
    Flp polyclonal antibody SDS JP ja Download
    Flp polyclonal antibody SDS BE nl Download
    Flp polyclonal antibody SDS BE fr Download
    Flp polyclonal antibody SDS FR fr Download
    Flp polyclonal antibody SDS ES es Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Flp polyclonal antibody (Sample Size) (Diagenode Cat# C15310169-20 Lot# A280-004). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Development of a lentivirus vector-based assay for non-destructive monitoring of cell fusion activity.
    Neshati Z, Liu J, Zhou G, Schalij MJ, de Vries AA
    Cell-to-cell fusion can be quantified by endowing acceptor and donor cells with latent reporter genes/proteins and activators of these genes/proteins, respectively. One way to accomplish this goal is by using a bipartite lentivirus vector (LV)-based cell fusion assay system in which the cellular fusion partners are ...

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