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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million HeLa cells using 15 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL1 antibody (upper right). The lower figure shows the gel image for the input, the negative IgG control and the ELAVL1 antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Whole cell extracts from 293T, HeLa, K562, Jurkat, NIH3T3, WR19L, Rat1, PC12 and CHO cells (lanes 1 to 9, respectively) were analysed by Western blot using the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 1.5 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238, lane 3). An equal amount of mouse IgG2a was used as a negative control (lane 2). Lane 1 shows the input. The immunoprecipitated ELAVL1 protein was subsequently detected by western blot with the ELAVL1 antibody as described above.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Whole cell extracts from 293T, HeLa, K562, Jurkat, NIH3T3, WR19L, Rat1, PC12 and CHO cells (lanes 1 to 9, respectively) were analysed by Western blot using the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 1.5 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238, lane 3). An equal amount of mouse IgG2a was used as a negative control (lane 2). Lane 1 shows the input. The immunoprecipitated ELAVL1 protein was subsequently detected by western blot with the ELAVL1 antibody as described above.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million HeLa cells using 15 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL1 antibody (upper right). The lower figure shows the gel image for the input, the negative IgG control and the ELAVL1 antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Whole cell extracts from 293T, HeLa, K562, Jurkat, NIH3T3, WR19L, Rat1, PC12 and CHO cells (lanes 1 to 9, respectively) were analysed by Western blot using the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 1.5 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238, lane 3). An equal amount of mouse IgG2a was used as a negative control (lane 2). Lane 1 shows the input. The immunoprecipitated ELAVL1 protein was subsequently detected by western blot with the ELAVL1 antibody as described above.</small></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million HeLa cells using 15 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL1 antibody (upper right). The lower figure shows the gel image for the input, the negative IgG control and the ELAVL1 antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 1.5 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238, lane 3). An equal amount of mouse IgG2a was used as a negative control (lane 2). Lane 1 shows the input. The immunoprecipitated ELAVL1 protein was subsequently detected by western blot with the ELAVL1 antibody as described above.</small></p>
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<p>Monoclonal antibody raised in mouse against human ELAVL1 (ELAV Like RNA Binding Protein 1).</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million HeLa cells using 15 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL1 antibody (upper right). The lower figure shows the gel image for the input, the negative IgG control and the ELAVL1 antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Whole cell extracts from 293T, HeLa, K562, Jurkat, NIH3T3, WR19L, Rat1, PC12 and CHO cells (lanes 1 to 9, respectively) were analysed by Western blot using the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 1.5 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238, lane 3). An equal amount of mouse IgG2a was used as a negative control (lane 2). Lane 1 shows the input. The immunoprecipitated ELAVL1 protein was subsequently detected by western blot with the ELAVL1 antibody as described above.</small></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million HeLa cells using 15 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL1 antibody (upper right). The lower figure shows the gel image for the input, the negative IgG control and the ELAVL1 antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Whole cell extracts from 293T, HeLa, K562, Jurkat, NIH3T3, WR19L, Rat1, PC12 and CHO cells (lanes 1 to 9, respectively) were analysed by Western blot using the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 1.5 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238, lane 3). An equal amount of mouse IgG2a was used as a negative control (lane 2). Lane 1 shows the input. The immunoprecipitated ELAVL1 protein was subsequently detected by western blot with the ELAVL1 antibody as described above.</small></p>
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'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:23:07',
'created' => '2015-07-08 13:46:50',
'locale' => 'zho'
)
$description = '<p>Immunoprecipitation</p>'
$name = 'IP'
$document = array(
'id' => '1010',
'name' => 'ELAVL1 monoclonal antibody Datasheet',
'description' => '<p>Monoclonal antibody raised in mouse against human ELAVL1 (ELAV Like RNA Binding Protein 1).</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/elavl1-monoclonal-antibody-100-ul.pdf',
'slug' => 'elavl1-monoclonal-antibody-tds',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-07-03 16:19:13',
'created' => '2018-07-03 16:19:13',
'ProductsDocument' => array(
'id' => '2648',
'product_id' => '2972',
'document_id' => '1010'
)
)
$sds = array(
'id' => '1551',
'name' => 'ELAVL1 antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/ELAVL1/SDS-C15200238-ELAVL1_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2021-08-30 16:16:16',
'created' => '2021-08-30 16:16:16',
'ProductsSafetySheet' => array(
'id' => '2702',
'product_id' => '2972',
'safety_sheet_id' => '1551'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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