Diagenode

ELAVL1 monoclonal antibody

カタログ番号
フォーマット
価格
C15200238
100 µg
$315.00
  Bulk order



Monoclonal antibody raised in mouse against human ELAVL1 (ELAV Like RNA Binding Protein 1).

Lot001
Concentration1 µg/µl
Species reactivityHuman, mouse, rat, hamster: positive. Other species: not tested.
TypeMonoclonal
PurityPurified monoclonal antibody.
HostMouse
Storage ConditionsStore at -20°C.
Storage BufferPBS containing 50% glycerol, does not contain a preservative.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
RIP 15 µg per 107 cells Fig 1
Western blotting 1:1,000 Fig 2
IP 1.5 µg per 1.5x105 cells Fig 3
  • Validation data

    ChIP

    RIP

    Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1
    Immunoprecipitation was performed on total RNA isolated from 10 million HeLa cells using 15 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL1 antibody (upper right). The lower figure shows the gel image for the input, the negative IgG control and the ELAVL1 antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.

    WB

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against ELAVL1
    Whole cell extracts from 293T, HeLa, K562, Jurkat, NIH3T3, WR19L, Rat1, PC12 and CHO cells (lanes 1 to 9, respectively) were analysed by Western blot using the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    IP

    Figure 3. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1
    Immunoprecipitation was performed on whole cell extracts from HeLa cells using 1.5 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238, lane 3). An equal amount of mouse IgG2a was used as a negative control (lane 2). Lane 1 shows the input. The immunoprecipitated ELAVL1 protein was subsequently detected by western blot with the ELAVL1 antibody as described above.

  •  実験手法
    RIP
    RIP Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
  •  資料
    ELAVL1 monoclonal antibody Datasheet DATASHEET
    Monoclonal antibody raised in mouse against human ELAVL1 (ELAV Like RNA Binding Protein 1).
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: ELAVL1 monoclonal antibody (Diagenode Cat# C15200238 Lot# 001). Click here to copy to clipboard.

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