Cre polyclonal antibody

100 µl
  Bulk order

Polyclonal antibody raised in rabbit against Cre recombinase using a KLH-conjugated synthetic peptide located at the C-terminal part of the protein.

Concentrationnot determined
Species reactivity
PurityWhole antiserum
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 Fig 1
Western Blotting 1:500 Fig 2, (1)
  • Validation Data


    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Cre (cat. No. CS-168-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,900.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against Cre (1)
    Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Cre (cat. No. CS-168-100), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 38 KD) is indicated on the right.

  •  应用
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  文档
    Datasheet Cre CS-168-100 DATASHEET
    Datasheet description
  •  Safety sheets
    SDS C15310168 Cre Antibody GB en Download
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Cre polyclonal antibody (Diagenode Cat# C15310168 Lot# A1040-004). Click here to copy to clipboard.

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