Protocols

Protocols

Download Activity Test - Procedure for immunoprecipitation of native chromatin proteins
Proteins play role in almost all cellular processes, and the study of their activity is crucial for understanding these mechanisms. Slimane AIT-SI-ALI and his “epigenetics and cell fate&rdquo...

Download AMPure® XP Size Selection - IP-Star Compact
The “AMPure XP Size Selection” protocol on the IP-Star® is using the standard “Agencourt® AMPure® XP Beads” from Agencourt®. It allows you to perform size se...

Download AMPure® XP Size Selection - IP-Star® Compact -
The “AMPure_XP_Size_Selection” protocol on the IP-Star® is using the standard “Agencourt® AMPure® XP Beads” from Agencourt®. It allows you to perform size se...

Download Bacterial Cell Disruption using Bioruptor® Standard, Plus or XL
For cell lysis, we highly recommend using 1.5 ml TPX microtubes (Cat. No. C30010010) or 10 ml tubes (Cat. No. C30010012) and the corresponding tube holders (Cat. No. B01200011 and B01200012, respec...

Download ChIP - Just 4 steps to foolproofing fragmentation for ChIP using Bioruptor^® Standard, Plus or Pico
Just follow these four steps for shearing chromatin, and you’ll be well on your way to excellent ChIP results without the troubleshooting headache.

Download ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus
A review of the critical points for chromatin shearing.

Download ChIP from plant material with the LowCell# ChIP Kit
As preparation of plant material for Chromatin Immunoprecipiation differs essentially from preparation of culture cells, the LowCell# ChIP protocol has to be adapted respectively. We recommend ther...

Download ChIP from tissues with the LowCell# ChIP kit
In theory, you could use 30 mg of tissue per antibody per ChIP. You will start by optimizing conditions for the type of tissue you work with. The exact amount of tissue to start with depends on the...

Download ChIPettor Protocols
ChIPettor™ System for Histones and Transcription Factors. These protocols have been optimised for 1M cells per IP for Histones and 4 M cells per IP for Transcription Factors, although it is ...

Download Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor^® Pico
Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in cells and tissues. The assay can be cumbersome and the success is highly dependent on the quality of ...

Download Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor^® Standard, Plus or Pico
This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq ki...

Download Chromatin Shearing in Yeast Cells with Bioruptor® Standard and Plus
Standard procedure to shear Chromatin from Yeast.

Download Chromatin shearing with the Bioruptor Pico 10 µl - 300 µl
HeLa cells are fixed with 1% molecular grade formaldehyde (for 8 min at room temperature). Nucleus isolation is performed using buffers and reagents of Diagenode’s Chromatin Shearing Optimiza...

Download Chromatin shearing with the Bioruptor^® Pico 500 µl - 2 ml
Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShear kit - Low SDS (previous name: Chromatin Shearing Optimization kit – Low SDS) protocol (Cat. ...

Download Chromatin shearing with the Bioruptor® Pico, 0.2 ml Bioruptor® Microtubes and the corresponding holder
The first critical step of a successful ChIP experiment is the preparation of sheared chromatin with a suitable fragment range which best represents the biological scenario being investigated. The ...

Download CRISPR/Cas9 editing: mutation detection with mismatch cleavage assay
Genome editing using CRISPR/Cas9 is used for targeted mutagenesis. But because genome editing does not target all loci with similar efficiencies, the mutation hit-rate at a given locus needs to be ...

Download Deparaffinization - Procedure for FFPE nucleic acid extraction with the Bioruptor®
Deparafinization of FFPE samples is typically performed using a non-polar solvent, such as xylene, or a mineral oil-based method which can be time consuming and messy. Claire Josse and colleagues f...

Download DNA Shearing for Bioruptor^® Pico (2013-2019) - Standard 100 μl Volume
Standard protocols for programmable DNA size distributions, excellent reproducibility, and high dsDNA yields with Bioruptor® Pico.

Download DNA Shearing for Bioruptor® Pico (2013-2019) - 50 μl Volume
For DNA shearing in 50 μl we highly recommend to use the 0.1 ml tube holder & tube adaptors (Cat. No. B01200041) and the corresponding 0.1 ml Bioruptor® Microtubes (Cat. No. C30010015).

Download DNA Shearing for Bioruptor® Standard and Plus
For DNA shearing we highly recommend to use the tube holder for 0.5/0.65 ml tubes (Cat. No. UCD-pack 0.5) and the corresponding Bioruptor® 0.5 ml Microtubes for DNA Shearing (Cat. No. WA-004-05...

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