Diagenode

MycK323ac polyclonal antibody

Catalog Number
Format
Price
C15410346
50 μg/63 μl
$245.00
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Other names: v-Myc, c-Myc, MRTL, BHLHE39

Polyclonal antibody raised in rabbit against Myc (v-myc myelocytomatosis viral oncogene homolog) acetylated at lysine 323 (MycK323ac) using a KLH-conjugated synthetic peptide.

LotA506-0041
Concentration0.8 μg/μl
Species reactivityHuman: positive. Other species: not tested.
TypePolyclonal
PurityAffinity purified polyclonal antibody.
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide and 0.05% ProClin 300.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 - 1:500 Fig 1
IF 1:500 Fig 2
  • Validation data

    ELISA

    Figure 1. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MycK323ac (Cat. No. C15410346). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:8,000.

    IF

    Figure 2. Immunofluorescence using the Diagenode antibody directed against MycK323ac
    HeLa cells were stained with the Diagenode antibody against MycK323ac (cat. No. C15410346) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the MycK323ac antibody (middle) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  •  実験手法
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  •  資料
    MycK323ac polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against Myc (v-myc myelocytomatosis viral oncogene homolog) ...
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  •  Safety sheets
    MycK323ac Antibody SDS GB en Download
    MycK323ac Antibody SDS US en Download
    MycK323ac Antibody SDS DE de Download
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    MycK323ac Antibody SDS BE nl Download
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: MycK323ac polyclonal antibody (Diagenode Cat# C15410346 Lot# A506-0041). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Pathologic HDAC1/c-Myc signaling axis is responsible forangiotensinogen transcription and hypertension induced by high-fat diet.
    Youn E.K. et al.
    High-fat diet (HFD)-induced obesity is a cause of resistant hypertension. We have shown a possible link between histone deacetylases (HDACs) and renal angiotensinogen (Agt) upregulation in the HFD-induced hypertension, whereas the underlying mechanisms remain to be elucidated. Here, using a HDAC1/2 inhibitor romidep...

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  • Symposium: "Signaling through Chromatin"
    Grenoble, France
    Oct 2-Oct 4, 2023
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    Heidelberg, Germany
    Oct 11-Oct 14, 2023
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    Crete, Greece
    Oct 15-Oct 20, 2023
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