| ELISA Enzyme-linked immunosorbent assay. Read more |
Other names: v-Myc, c-Myc, MRTL, BHLHE39
Polyclonal antibody raised in rabbit against Myc (v-myc myelocytomatosis viral oncogene homolog) acetylated at lysine 323 (MycK323ac) using a KLH-conjugated synthetic peptide.
| Lot | A506-0041 |
|---|---|
| Concentration | 0.8 μg/μl |
| Species reactivity | Human: positive. Other species: not tested. |
| Type | Polyclonal |
| Purity | Affinity purified polyclonal antibody. |
| Host | Rabbit |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 - 1:500 | Fig 1 |
| IF | 1:500 | Fig 2 |
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MycK323ac (Cat. No. C15410346). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:8,000.
Figure 2. Immunofluorescence using the Diagenode antibody directed against MycK323ac
HeLa cells were stained with the Diagenode antibody against MycK323ac (cat. No. C15410346) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the MycK323ac antibody (middle) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
 How to properly cite this product in your workDiagenode strongly recommends using this: MycK323ac polyclonal antibody (sample size) (Diagenode Cat# C15410346-10 Lot# A506-0041). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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