Infinium Mouse Methylation Array Service

End-to-end array 

• Bisulfite conversion
• Whole genome amplification
• Array hybridization
• Single base extension  
• Array scanner 

Analysis

Features

Standard

Standard files provided:

• Sample annotation
• Variable annotation
• Scanner output (IDAT files)

Differential methylation analysis

Identification of differentially methylated CpGs between sample groups.

Files provided:

• Report with summary of differential methylation analysis and plots
• File containing the differentially methylated CpGs and breakdown of those positions in regional analysis (CpG islands, shelves, shores and open sea)
• File containing differential methylated regions (DMRs)

Gene ontology terms analysis

Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.

Pathway analysis

Identify biochemical pathways in which genes associated with differentially methylated regions (or individual differentially methylated CpGs) may be overrepresented.

Antagonizing epigenetically controlled PAF/PAF-R pathway improves liver function during experimental cirrhosis
Ángel-Gomis, Enrique et al.
Background and aims Platelet-activating factor (PAF) phospholipid is mainly produced by macrophages and involved in pro-inflammatory responses. We evaluated the regulation of PAF-R gene expression during experimental cirrhosis and whether antagonizing its ligand PAF improves liver function and inflammation. Method...