Request a quote for a bulk order for H3K9ac monoclonal antibody (sample size). Please fill out the form here below. Your local sales account manager will get in touch with you
shortly and send you a quotation based on your requirements.
Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K9ac ChIP assays were performed using human HeLa cells, the Diagenode monoclonal antibody against H3K9ac (Cat. No. C15200185) and optimized PCR primer pairs for qPCR. ChIP was performed with the “Auto Histone ChIP-seq” kit (Cat. No. C01010020), using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the EIF4A2 gene, used as positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against H3K9ac To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against H3K9ac (Cat. No. MAb-185-050). The wells were coated with peptides containing the unmodified H3K9 region as well as the acetylated H3K9 and the acetylated H3K27. Figure 1 shows a high specificity of the antibody for the peptide containing the modification of interest.
Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against H3K9ac HeLa cells were stained with the Diagenode antibody against H3K9ac (Cat. No. MAb-185-050) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9ac antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
Learn more about: Load... Read more
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
HeLa cells transfected with a Cas9 expression vector (... Read more
Citrate modulates lipopolysaccharide-induced monocyte inflammatory responses. Ashbrook MJ, McDonough KL, Pituch JJ, Christopherson PL, Cornell TT, Selewski DT, Shanley TP, Blatt NB Citrate, a central component of cellular metabolism, is a widely used anti-coagulant due to its ability to chelate calcium. Adenosine triphosphate (ATP)-citrate lyase, which metabolizes citrate, has been shown to be essential for inflammation, but the ability of exogenous citrate to impact inflammatory signalling ca...
A novel mechanism for CTCF in the epigenetic regulation of Bax in breast cancer cells. Méndez-Catalá CF, Gretton S, Vostrov A, Pugacheva E, Farrar D, Ito Y, Docquier F, Kita GX, Murrell A, Lobanenkov V, Klenova E. We previously reported the association of elevated levels of the multifunctional transcription factor, CCCTC binding factor (CTCF), in breast cancer cells with the specific anti-apoptotic function of CTCF. To understand the molecular mechanisms of this phenomenon, we investigated regulation of the human Bax gene by ...