Diagenode

ATAC-seq kit

Catalog Number
Format
Price
C01080001
8 rxns
$615.00
Other format

ATAC-seq (Assay for Transposase-Accessible Chromatin) とその後に行われる次世代シーケンシングは、ゲノムワイドにアクセス可能なクロマチンを特例するのに重要な技術です。 この技術は、トランスポザーゼTn5を用いる事により露出したオープンクロマチンを切断、同時にアダプターをライゲーションして、その後の増幅とシーケンシングを行います。 ATAC-seqメソッドにより以下の事が可能です。

  •     遺伝子調節の洞察を得る事で、オープンクロマチンシグネチャーを理解する
  •     一塩基分解能でヌクレオソームの位置を決定
  •     転写因子(TF)の占有率を明確にする

反応あたり50,000細胞用に最適化されたDiagenodeのATAC-seqキットは、高度に検証されたプロトコールに基づきます。 またキットには細胞溶解、核抽出、タグ付け、DNA精製、およびライブラリー増幅用の試薬が含まれています。 多重化用のプライマーインデックスは別途購入していただく必要があります。

ATAC-seqキットの特徴:

  • 細胞要件:50,000セル / rxn
  • 反復実験と反復実験の間で高い再現性を備えた堅牢なプロトコール
  • DiagenodeのMicroChIPDiaPureColumns(付属)を使用したタグ付け反応後の簡単で効率的なDNAキャプチャー
  • ライブラリー増幅に必要なサイクル数の決定に不可欠なボーナスqPCRステップ:  
    • 過剰増幅を回避
    • より困難なサンプルにも適応/柔軟性がライブラリー調製の成功を実現
    • 機能しない実験の(qPCR増幅なし)早期発見

  • メソッドの概要

    ATAC-seq, Assay for Transposase-Accessible Chromatin, followed by next generation sequencing, is a key technology to easily identify the open regions of the chromatin. The protocol consists of 3 steps: nuclei preparation, tagmentation and library amplification. First, the cells undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which  cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.

    ATAC-seq kit workflow

  • 結果の例

    library prepared with the Diagenode ATAC-seq kit

    Figure 1.Representative Bioanalyzer profile of an ATAC-seq library prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells.

    Diagenode ATAC-seq kit

    Figure 2. Main ATAC-seq alignment and peak calling statistics of 3 replicates prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells. (Mapping efficiency: Percentage of non-mitochondrial reads that mapped to the reference genome. Uniquely mapped ratio: Proportion of mapped reads that map to only one location on the reference genome (hg19). Peaks: Number of peaks (open chromatin regions) identified by MACS2 for each sample. FRiP - Fraction of reads in peaks: Percentage of reads in peaks, with respect to the number of uniquely mapped reads. Sequencing was realized in paired-end mode 50 base pairs (PE50) on an Illumina NovaSeq6000.)

    Assay for Transposase-Accessible Chromatin

    Assay for Transposase-Accessible Chromatin

    Figure 3 Sequencing profiles of ATAC-seq library (3 replicates) prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells.

     open chromatin regions

    Figure 4.
    Heatmap around TSS of three ATAC-seq replicates prepared with the Diagenode ATAC-seq kit and 24 UDI for tagmented libraries (Cat. No. C01011034) on 50,000 nuclei from K562 cells.

  • ATAC-seqキット専用の追加ソリューション
  •  資料
    ATAC-seq Kit - Manual MANUAL
    Gene expression is carefully regulated in the cells in order to manage a wide range of biological...
    Download
    Chromatin Brochure BROCHURE
    Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has e...
    Download
  •  Safety sheets
    ATAC-seq kit SDS GB en Download
    ATAC-seq kit SDS US en Download
    ATAC-seq kit SDS BE nl Download
    ATAC-seq kit SDS BE fr Download
    ATAC-seq kit SDS FR fr Download
    ATAC-seq kit SDS ES es Download
    ATAC-seq kit SDS DE de Download
    ATAC-seq kit SDS JP ja Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: ATAC-seq kit (Diagenode Cat# C01080001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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    Dynamic PRC1-CBX8 stabilizes a porous structure of chromatin condensates
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