CATS Small RNA-seq Kit x24

Catalog Number
24 rxns

Diagenodeの「CATS small RNA-seq Kit」は、革新的なCATS法(Capture and Amplification by Tailing and Switching)を採用することで、ライゲーションが不要でかつ低インプットRNA量から次世代シークエンシングのDNAライブラリーを作製することができるキットです。

  • 多様なスモールRNAを回収可能
  • 10pgの超少量インプットRNAで優れた再現性
  • 難しいサンプルでの優れたパフォーマンス
  • 続きを読む

    CATSは、RNAの3’末端をキャプチャーするためのポリヌクレオチドテーリングおよび、cDNA合成時に5’末端をキャプチャーするためのMMLV-RTによるテンプレートスイッチ 能力を利用した技術です。この方法なら、10ピコグラムの低インプットRNA量からわずか数時間でIllumina用シークエンシングライブラリーを作製することができます。

    ライブラリーは、ストランド特異的な情報を保持しています。「CATS RNA-seq Kit」は、増幅を最小限に抑えることでより多くの有効な鋳型をキャプチャーし、また、バイアスを最小限に抑えることで高い複雑性を得ることができるので、他のライゲーションベースの手法と比べより高いライブラリー作製効率を示します。

Unique sncRNAs detected in circulating plasma RNA

sncRNA transcripts detected using the CATS small RNA-seq Kit vs the Competitor’s Kit. The input was 1 ng circulating small RNA in plasma samples. In total, 908 transcripts were found in the CATS small RNA-seq libraries. TPM≥1

PCR cycles: CATS 14x vs Competitor 21x. Final library yield: CATS 27 ng vs Competitor 1.2 ng.

More miRNA transcripts detected from challenging samples

Transcripts detected in miRNA samples of CATS small RNA-seq Kit vs the Competitor’s Kit.

Input: 1 ng circulating small RNA in plasma samples. PCR cycles: CATS 14x vs Competitor 21x. Final library yield: CATS 27 ng vs Competitor 1.2 ng.

Ultra low inputs, excellent reproducibility

Correlation between 2 different operators using CATS small RNA-seq Kit on two different inputs (100 pg vs 1 ng) for ncRNA detected at TPM ≥2.
R2 = 0.86

Consistency across template switching techniques

Comparison of the number of detected small non-coding RNAs CATS small RNA-seq Kit vs SMARTer
Input: 100 pg isolated small RNA. TPM ≥2. Transcripts taken into consideration: miRNA, piRNA, scRNA, scaRNA, snoRNA, snRNA and vaultRNA. 1,217 transcripts found in both libraries at this expression level.

High number of reads mapped

Representation of detected transcripts different biotypes with CATS small RNA-seq Kitvs NEBNext UltraI kit. TPM≥2.
100 ng total RNA, 1 ng small RNA used as input.

Excellent performance on challenging samples

Heatmap clustering of the top 50 differentially expressed miRNAs after library preparation:

  • CATS Small RNA-seq Kit on 1 ng exosomal RNA (Exosome sample 1, 2)
  • CATS RNA-seq Kit on 1 ng total RNA from parent (HEK293T) cells (Cell sample 1, 2).

Prior library preparation, exosomes were isolated with Diagenode exosome capture solutions Exosomes

CATS saves time

  • 特徴
  • FAQs
  •  資料
    D-Plex Small RNA-seq Kit for Illumina MANUAL
    Note: The D-Plex Small RNA-seq Kit manual describes the protocol for both UDI and SI li...
    D-Plex Small RNA DNBSEQ™ Kit for MGI MANUAL
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: CATS Small RNA-seq Kit x24 (Diagenode Cat# C05010040). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Functional microRNA targetome undergoes degeneration-induced shift in the retina
    Chu-Tan JA et al.
    Background: MicroRNA (miRNA) play a significant role in the pathogenesis of complex neurodegenerative diseases including age-related macular degeneration (AMD), acting as post-transcriptional gene suppressors through their association with argonaute 2 (AGO2) - a key member of the RNA Induced Silencing Complex...

    Repeat RNAs associate with replication forks and post-replicative DNA.
    Gylling HM, Gonzalez-Aguilera C, Smith MA, Kaczorowski DC, Groth A, Lund AH
    Non-coding RNA has a proven ability to direct and regulate chromatin modifications by acting as scaffolds between DNA and histone-modifying complexes. However, it is unknown if ncRNA plays any role in DNA replication and epigenome maintenance, including histone eviction and re-instalment of histone-modifications aft...

    Multisite Evaluation of Next-Generation Methods for Small RNA Quantification.
    Herbert ZT, Thimmapuram J, Xie S, Kershner JP, Kolling FW, Ringelberg CS, LeClerc A, Alekseyev YO, Fan J, Podnar JW, Stevenson HS, Sommerville G, Gupta S, Berkeley M, Koeman J, Perera A, Scott AR, Grenier JK, Malik J, Ashton JM, Pivarski KL, Wang X, Kuffe
    Small RNAs (smRNAs) are important regulators of many biologic processes and are now most frequently characterized using Illumina sequencing. However, although standard RNA sequencing library preparation has become routine in most sequencing facilities, smRNA sequencing library preparation has historically been chall...

    Circulating miRNA analysis for cancer diagnostics and therapy.
    Valihrach L, Androvic P, Kubista M
    Successful treatment of cancer depends on early diagnosis and effective monitoring of patients' response to therapy. Traditional tools based on tumor biopsies lack the sensitivity and specificity to capture cancer development in its early phases and are not applicable for continuous monitoring. To overcome these bar...

    The sncRNA Zoo: a repository for circulating small noncoding RNAs in animals.
    Fehlmann T, Backes C, Pirritano M, Laufer T, Galata V, Kern F, Kahraman M, Gasparoni G, Ludwig N, Lenhof HP, Gregersen HA, Francke R, Meese E, Simon M, Keller A
    The repertoire of small noncoding RNAs (sncRNAs), particularly miRNAs, in animals is considered to be evolutionarily conserved. Studies on sncRNAs are often largely based on homology-based information, relying on genomic sequence similarity and excluding actual expression data. To obtain information on sncRNA expres...

    MicroRNAs and Transplantation.
    Khan Z, Suthanthiran M, Muthukumar T
    miRNAs, ∼20 to 22 nucleotide single-stranded RNA species that play a pivotal role in the regulation of protein-coding genes, are emerging as robust biomarkers for assessing allograft status. Herein, the authors briefly review the biogenesis and function of the miRNAs and provide an overview of the tools to quant...

    NGS analysis of total small non coding RNAs from low input RNA from dried blood sampling
    Marcello Pirritano, Tobias Fehlmann, Thomas Laufer, Nicole Ludwig, Gilles Gasparoni, Yongping Li, Eckart Meese, Andreas Keller, and Martin Simon
    Circulating miRNAs are favored for biomarker candidates as they can reflect tissue specific miRNA dysregulation in disease contexts. Moreover, they have additional advantages that they can be monitored in a minimal invasive manner. Blood-borne miRNAs are therefore currently characterized to identify, describe and va...

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