Diagenode

ZMYM3 Antibody - ChIP-seq Grade (sample size)

目录号
格式
价格
C15200016-10
10 µg
$115.00
  Bulk order
其他格式



Monoclonal antibody raised in mouse against human ZMYM3 (Zinc Finger, MYM-Type 3), using the full length recombinant protein. 

Lot001
Concentration2.07 μg/μl
Species reactivityHuman
TypeMonoclonal
PurityProtein A purified monoclonal antibody in PBS containing 0.05% azide.
HostMouse
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP /ChIP-seq * 1-2 μg/ChIP Fig 1, 2
Protein array 1:100,000 Fig 3
IF 1:1,000 Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.

  • Validation data

    ZMYM3 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against ZMYM3
    ChIP was performed using HepG2 cells, the Diagenode monoclonal antibody against ZMYM3 (Cat. No. C15200016) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers near the CAMK2N1 and SCML1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ZMYM3 Antibody ChIP-seq Grade

    ZMYM3 Antibody for  ChIP-seq

    ZMYM3 Antibody  ChIP-seq assay

    ZMYM3 Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against ZMYM3
    ChIP was performed with 1 μg of the Diagenode antibody against ZMYM3 (Cat. No. C15200016) on sheared chromatin from 4 million HepG2 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 2 Mb region of chromosome 3 (figure 2A and B) and in two regions surrounding the SCML1 and CAMK2N1 positive control genes (figure 2C and D). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.

    ZMYM3 Antibody validated in Protein array

    Figure 3. Protein array analysis with the Diagenode antibody directed against ZMYM3
    The specificity of the Diagenode antibody against ZMYM3 (Cat. No. C15200016) was demonstrated using the HuProt human protein microarray (CDI Laboratories), a protein array containing more than 19,000 human proteins. The antibody was used at a dilution of 1:100,000. Figure 4 shows the Z-score of the signal intensity (mean value of the duplicate spots on the array). The names of the proteins with 3 highest Z-scores are indicated at the bottom. This figure clearly shows the high specificity of the antibody for ZMYM3.

    ZMYM3 Antibody validated in Immunofluorescence

    Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against ZMYM3
    HeLa cells were stained with the Diagenode monoclonal antibody against ZMYM3 (cat. No. C15200016) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the ZMYM3 antibody (middle) diluted 1:1.000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  • Target Description

    Monoclonal antibody raised in mouse against human ZMYM3 (Zinc Finger, MYM-Type 3), using the full length recombinant protein.

  •  应用
  •  文档
    ZMYM3 monoclonal antibody C15200016 DATASHEET
    Datasheet of the ZMYM3 monoclonal antibody
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  •  Safety sheets
    ZMYM3 Antibody SDS GB en Download
    ZMYM3 Antibody SDS US en Download
    ZMYM3 Antibody SDS BE nl Download
    ZMYM3 Antibody SDS BE fr Download
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    ZMYM3 Antibody SDS ES es Download
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    ZMYM3 Antibody SDS JP ja Download
  •  出版物

    How to properly cite this product in your work

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  • Long-Read Sequencing Meeting 2024
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    Oct 21-Oct 23, 2024
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