Diagenode

H3T3p Antibody - ChIP Grade (sample size)

目录号
格式
价格
C15210001-10
10 µg
$115.00
  Bulk order
其他格式



Monoclonal antibody raised in rabbit against histone H3, phosphorylated at threonine 3 (H3T3p), using a KLH-conjugated synthetic peptide.

Lot001
Concentration1 μg/μl
Species reactivityHuman
TypeMonoclonal
PurityProtein A purified
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP* 0.5 - 1 μg/ChIP Fig 1
Western Blotting 1:10,000 Fig 2
Immunofluorescence 1:1,000 Fig 3

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 µg per ChIP.

  • Validation Data

    H3T3p Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3T3p
    ChIP assays were performed using HeLa cells, the Diagenode antibody against H3T3p (cat. No. C15210001) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010051), using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the TSH2B and HBB genes, used as positive controls, and for the promoters of the EIF4A2 and GAPDH genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    H3T3p Antibody validated in Western blot

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against H3T3p
    Histone extracts from HeLa cells (lane 1) or HeLa cells treated with nocodazole (lane 2), as well as recombinant H3 (lane 3) were analysed by Western blot using the Diagenode monoclonal antibody against H3T3p (Cat. No. C15210001) diluted 1:10,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    H3T3p Antibody validated in Immunofluorescence

    Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against H3T3p
    HeLa cells were stained with the Diagenode antibody against H3T3p (Cat. No. C15210001, red) diluted 1:1,000. Actin was stained with fluorescein phalladoin (green).

  • Target Description

    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.

  •  应用
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-qPCR (ab)
    Read more
  •  文档
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    H3T3p monoclonal antibody DATASHEET
    Monoclonal antibody raised in rabbit against histone H3, phosphorylated at threonine 3 (H3T3p), u...
    Download
  •  Safety sheets
    H3T3p Antibody SDS US en Download
    H3T3p Antibody SDS GB en Download
    H3T3p Antibody SDS BE fr Download
    H3T3p Antibody SDS FR fr Download
    H3T3p Antibody SDS ES es Download
    H3T3p Antibody SDS DE de Download
    H3T3p Antibody SDS JP ja Download
    H3T3p Antibody SDS BE nl Download
  •  出版物

    How to properly cite this product in your work

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