Diagenode

>>>   Click for Diagenode’s approach to COVID-19

H3K36me2 Antibody - ChIP Grade (sample size)

目录号
格式
价格
C15200182-10
(MAb-182-050)
10 µg
$97.00
  Bulk order
其他格式



Monoclonal antibody raised in mouse against histone H3 dimethylated at lysine 36 (H3K36me2), using a KLH-conjugated synthetic peptide.

Lot001-12
Concentration1.0 µg/µl
Species reactivityHuman: positive. Other species: not tested.
TypeMonoclonal
PurityProtein A purified monoclonal antibody.
HostMouse
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 1-2 µg/ChIP Fig 1
ELISA 1:3,000 Fig 2
Dot Blotting 1:10,000
Western Blotting 1:1,000 - 1:2,000

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.

  • Validation Data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K36me2
    ChIP assays were performed using human HeLa cells, the Diagenode monoclonal antibody against H3K36me2 (Cat. No. C15200182) and optimized PCR primer pairs for qPCR. ChIP was performed with the “Auto Histone ChIP-seq” kit (Cat. No. C01010020), using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (1 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a genomic region upstream of the TGM2 gene, used as a positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ELISA

    Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against H3K36me2
    To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against H3K36me2 (Cat. No. C15200182). The wells were coated with peptides containing the unmodified H3K36 region as well as the mono-, di- and trimethylated H3K36 and the trimethylated H3K9. Figure 2 shows a high specificity of the antibody for the peptide containing the modification of interest.

  •  应用
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    DB
    Dot blotting Read more
    ChIP-qPCR (ab)
    Read more
  •  文档
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    Datasheet H3K36me2 MAb-182-050 DATASHEET
    Datasheet description
    Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H3K36me2 Antibody - ChIP Grade (sample size) (Diagenode Cat# C15200182-10 Lot# 001-12). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    A histone H3.3K36M mutation in mice causes an imbalance of histonemodifications and defects in chondrocyte differentiation.
    Abe, Shusaku and Nagatomo, Hiroaki and Sasaki, Hiroyuki and Ishiuchi,Takashi
    Histone lysine-to-methionine (K-to-M) mutations have been identified as driver mutations in human cancers. Interestingly, these 'oncohistone' mutations inhibit the activity of histone methyltransferases. Therefore, they can potentially be used as versatile tools to investigate the roles of histone modifications. In ...

    A lncRNA regulates alternative splicing via establishment of a splicing-specific chromatin signature.
    Gonzalez I, Munita R, Agirre E, Dittmer TA, Gysling K, Misteli T, Luco RF
    Alternative pre-mRNA splicing is a highly cell type-specific process essential to generating protein diversity. However, the mechanisms responsible for the establishment and maintenance of heritable cell-specific alternative-splicing programs are poorly understood. Recent observations point to a role of histone modi...

活动

  • DNA methylation workshop: Become an expert at RRBS
    Diagenode
    Oct 1-Oct 31, 2021
 查看所有活动

新闻

 查看所有新闻


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.



  ABOUT SSL CERTIFICATES

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics