Application Note - Mapping Histone post-translational modifications in the Pacific oyster using iDeal CUT&Tag and the Tissue Nuclei Extraction Module.
Mollusks represent one of the largest and most diverse phyla in the animal kingdom (Escoubas et al., 2016). Nevertheless, the different carriers of epigenetic information have only rarely been studied in these species. This lack of knowledge is particularly concerning for Magallana gigas, commonly known as the Pacific Oyster, which is the most widely distributed bivalve mollusk species in aquaculture worldwide (Botta et al., 2020). This gap is especially pronounced when it comes to the mapping of posttranslational histone modifications. We had anecdotal evidence that chromatin immunoprecipitation (ChIP) was particularly difficult to perform with mollusk species. To address this technical challenge, we tested different ChIP-seq methods (Dellong et al., 2024) and in our hands, CUT&Tag with the Tissue Nuclei Extraction module and the iDeal CUT&Tag kit for histones gave the most convincing results.
