Diagenode

Tissue Nuclei Extraction for ATAC-seq

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目录号
格式
价格
C01080003
8 rxns
$160.00
其他格式

Diagenode’s Tissue Nuclei Extraction for ATAC-seq module has been developed and validated for efficient nuclei extraction from tissue samples prior to ATAC-seq experiment. The protocol has been validated on many different tissues (lung, liver, brain, kidney, muscles) and different species (pork, chicken).

For a complete ATAC-seq for tissue workflow we highly recommend to use the ATAC-seq package for tissue. The ATAC-seq package includes all reagents needed for nuclei extraction from tissues, library preparation and multiplexing. For more details please go to the page: ATAC-seq package for tissue.

Features:

  • Starting amount: 25 mg – 100 mg per rxn
  • Validated on many mammalian tissues
  • Included in a complete ATAC-seq workflow: ATAC-seq package for tissue

Looking for ATAC-seq for cells? Please go to: ATAC-seq kit

  • Example of results

    ATAC-seq, Assay for Transposase-Accessible Chromatin, followed by next generation sequencing, is a key technology to easily identify the open regions of the chromatin. The protocol consists of 3 steps: nuclei preparation, tagmentation and library amplification. First, the tissue undergo the lysis, ending with the crude nuclei. Then, the nuclei are incubated with a tagmentase (Tn5 transposase), which  cuts the genomic regions associated with open chromatin and inserts the sequencing adaptors. Finally, the generated libraries are amplified and can be used for sequencing. High-throughput sequencing will then detect peaks, in open regions of the chromatin only, giving a map of the chromatin status in the whole genome of the sample.


    workflow

  • Example of results

    GENE-SWitCH aims to deliver new underpinning knowledge on the functional genomes of two main monogastric farm species (pig and chicken) and to enable immediate translation to the pig and poultry sectors. It is a multi-actor project that will produce new genome information to enable the characterization of genetic and epigenetic determinants of complex traits in these two species. Diagenode, as a principal participant to the project and leading the WP1, developed a new protocol to improve the preparation of ATAC-seq libraries from a variety of snap-frozen tissues. The ATAC-seq protocol combines efficient nuclei extraction procedure validated on 7 different kinds of tissues from 3 developmental stages of the two species and a robust Tagmentation protocol based on Diagenode Tn5 enzyme. The developed ATAC-seq protocol was successfully used to produce 168 ATAC-seq libraries for WP1 and 320 for WP5.

    Table 1. List of validated tissues with Diagenode’s ATAC-seq package for tissue (Cat. No. C01080005/6). The samples were used as part of GENE-SWitCH consortium.

    A.

    B.

    Figure 2. ATAC-seq library profiles generated using the ATAC-seq package for tissue (Cat. No. C01080005/6) from pork’s liver (A) and brain (B). The samples were used as part of GENE-SWitCH consortium.

  •  文档
    Chromatin Brochure BROCHURE
    Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has e...
    Download
    ATAC-seq Kit - Manual MANUAL
    Gene expression is carefully regulated in the cells in order to manage a wide range of biological...
    Download
  •  Safety sheets
    Tissue Nuclei Extraction for ATAC-seq SDS US en Download
    Tissue Nuclei Extraction for ATAC-seq SDS GB en Download
    Tissue Nuclei Extraction for ATAC-seq SDS BE fr Download
    Tissue Nuclei Extraction for ATAC-seq SDS FR fr Download
    Tissue Nuclei Extraction for ATAC-seq SDS ES es Download
    Tissue Nuclei Extraction for ATAC-seq SDS DE de Download
    Tissue Nuclei Extraction for ATAC-seq SDS JP ja Download
    Tissue Nuclei Extraction for ATAC-seq SDS BE nl Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Tissue Nuclei Extraction for ATAC-seq (Diagenode Cat# C01080003). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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