Diagenode

Environmental enrichment induces epigenomic and genome organization changesrelevant for cognitive function


Espeso-Gil, S. et al.

In early development, the environment triggers mnemonic epigenomic programs resulting in memory and learning experiences to confer cognitive phenotypes into adulthood. To uncover how environmental stimulation impacts the epigenome and genome organization, we used the paradigm of environmental enrichment (EE) in young mice constantly receiving novel stimulation. We profiled epigenome and chromatin architecture in whole cortex and sorted neurons by deep-sequencing techniques. Specifically, we studied chromatin accessibility, gene and protein regulation, and 3D genome conformation, combined with predicted enhancer and chromatin interactions. We identified increased chromatin accessibility, transcription factor binding including CTCF-mediated insulation, differential occupancy of H3K36me3 and H3K79me2, and changes in transcriptional programs required for neuronal development. EE stimuli led to local genome re-organization by inducing increased contacts between chromosomes 7 and 17 (inter-chromosomal). Our findings support the notion that EE-induced learning and memory processes are directly associated with the epigenome and genome organization.

Tags
Antibody

Share this article

Published
February, 2021

Source

Products used in this publication

  • cut and tag antibody icon
    C15410210-50
    CTCF polyclonal antibody
  • cut and tag antibody icon
    C15410196
    H3K27ac polyclonal antibody
  • cut and tag antibody icon
    C15410194
    H3K4me1 polyclonal antibody
  • ChIP-seq Grade
    C15410192
    H3K36me3 polyclonal antibody
  • ChIP-seq Grade
    C15410051
    H3K79me2 polyclonal antibody
  • cut and tag antibody icon
    C15410193
    H3K9me3 polyclonal antibody
  • cut and tag antibody icon
    C15410195
    H3K27me3 polyclonal antibody

ニュース

 すべてのニュースを見る


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics