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Standardizing chromatin research: a simple and universal method for ChIP-seq


Laura Arrigoni, Andreas S. Richter, Emily Betancourt, Kerstin Bruder, Sarah Diehl, Thomas Manke and Ulrike Bönisch

Here we demonstrate that harmonization of ChIP-seq workflows across cell types and conditions is possible when obtaining chromatin from properly isolated nuclei. We established an ultrasound-based nuclei extraction method (Nuclei Extraction by Sonication) that is highly effective across various organisms, cell types and cell numbers. The described method has the potential to replace complex cell-type-specific, but largely ineffective, nuclei isolation protocols. This article demonstrates protocol standardization using the Bioruptor shearing systems and the IP-Star Automation System for ChIP automation.

Tags
Bioruptor
H3K4me3 (C15410003)
IP-Star Compact
H3K27me3 (C15410195)
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Published
December, 2015

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Products used in this publication

  • some alt
    B01020001
    Bioruptor® Plus sonication device
  • some alt
    B03000002
    SX-8G IP-Star® Compact Automated System
  • Antibody ChIP-seq grade icon
    C15410003-50
    H3K4me3 polyclonal antibody - Premium
  • Antibody ChIP icon
    C15310135
    H3pan polyclonal antibody - Classic
  • Antibody ChIP-seq grade icon
    C15410195
    H3K27me3 polyclonal antibody - Premium

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