Comprehensive analysis of DNA-methylation in mammalian tissues using MeDIP-chip.

Pälmke N, Santacruz D, Walter J

Genome-wide mapping of epigenetic changes is essential for understanding the mechanisms involved in gene regulation during cell differentiation and embryonic development. DNA-methylation is one of these key epigenetic marks that is directly linked to gene expression is. Methylated DNA immunoprecipitation (MeDIP) is a recently devised method used to determine the distribution of DNA-methylation within functional regions (e.g., promoters) or in the entire genome robustly and cost-efficiently. This approach is based on the enrichment of methylated DNA with an antibody that specifically binds to 5-methyl-cytosine and can be combined with PCR, microarrays or high-throughput sequencing. This article outlines the experimental procedure of MeDIP-chip and provides a comprehensive summary of quality control strategies and primary data analysis.

DNA shearing

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February, 2011


Products used in this publication

  • Mouse IgG
    5-methylcytosine (5-mC) monoclonal antibody cl. b



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