WDR5 polyclonal antibody

Catalog Number
100 µl
  Bulk order

Alternative names: BIG3, SWD3

Polyclonal antibody raised in rabbit against mouse Wdr5 (WD (tryptophan-aspartate) repeat domain protein 5), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.

Concentrationnot determined
Species reactivityMouse
PurityWhole antiserum
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 Fig 1
Western Blotting 1:1,000 Fig 2, (1)
  • Validation Data


    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Wdr5 (Cat. No. CS-101-100). By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:12,300.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against Wdr5 (1)
    Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Wdr5 (Cat. No. CS-101-100), diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (36 kDa) is indicated on the right.

  •  実験手法
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  資料
    Datasheet Wdr5 CS-101-100 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Safety sheets
    SDS C15310101 WDR5 Antibody GB en Download
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: WDR5 polyclonal antibody (Diagenode Cat# C15310101 Lot# A266-004 ). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Chd8 regulates X chromosome inactivation in mouse through fine-tuningcontrol of Xist expression.
    Cerase, Andrea et al.
    Female mammals achieve dosage compensation by inactivating one of their two X chromosomes during development, a process entirely dependent on Xist, an X-linked long non-coding RNA (lncRNA). At the onset of X chromosome inactivation (XCI), Xist is up-regulated and spreads along the future inactive X chromosome. Conte...

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