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Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against hSirT1 Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 μg) and the Diagenode monoclonal antibody against hSirT1 (cat# AC-063-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left), the position of the protein of interest is shown on the right.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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