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<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
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<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
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<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
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<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
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<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
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<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
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<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
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<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
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<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
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<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
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<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
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<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<td><span>RIP*</span></td>
<td><span>2 µg per IP</span></td>
<td>Fig 1, 2</td>
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<tr>
<td>Dot Blotting</td>
<td>1:500</td>
<td>Fig 3, 4</td>
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<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>',
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<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
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<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
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<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
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<td><span>RIP*</span></td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>',
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<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
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<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
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<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
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'label2' => '',
'info2' => '',
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'price_CNY' => '',
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$chipseq_service = array(
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$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
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'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 μg',
'catalog_number' => 'C15200082-10',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000582',
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'search_order' => '',
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'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'm6a-monoclonal-antibody-10-ug',
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'name' => 'DB',
'description' => '<p>Dot blotting</p>',
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'modified' => '2016-01-13 14:40:49',
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'name' => 'DB',
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'tabular' => true,
'slug' => 'dot-blotting',
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'modified' => '2016-01-13 14:40:49',
'created' => '2015-07-08 13:45:05',
'locale' => 'jpn'
)
$description = '<p>Dot blotting</p>'
$name = 'DB'
$document = array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
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'type' => 'Brochure',
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'authors' => 'Debo Brian M. et al.',
'description' => '<p>The detection of low-abundance DNA N6-methyladenine (DNA-m6A) remains challenging, limiting our understanding of this novel base in eukaryotes. To address this, we introduce an approach for systematically validating the selectivity and sensitivity of antibody-based DNA-m6A methods, revealing most commercial antibodies as poorly selective towards DNA-m6A. Finally, using a validated highly selective anti-DNA-m6A antibody we expose distinct patterns of DNA-m6A in C. reinhardtii, A. thaliana, and D. melanogaster.</p>',
'date' => '2022-03-01',
'pmid' => 'https://doi.org/10.1101%2F2022.03.02.482749',
'doi' => '10.1101/2022.03.02.482749',
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'created' => '2022-08-11 12:14:50',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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