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<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
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<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
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<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
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<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
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<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
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<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
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<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
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<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
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<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
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<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
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<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
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<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
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<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>',
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<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
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<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
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<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
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<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
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<th>Suggested dilution</th>
<th>References</th>
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<td><span>RIP*</span></td>
<td><span>2 µg per IP</span></td>
<td>Fig 1, 2</td>
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<td>1:500</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>',
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<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
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<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
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<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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