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Evaluation of N<sup>6</sup>-adenine DNA-immunoprecipitation-basedgenomic profiling in eukaryotes


Debo Brian M. et al.

The detection of low-abundance DNA N6-methyladenine (DNA-m6A) remains challenging, limiting our understanding of this novel base in eukaryotes. To address this, we introduce an approach for systematically validating the selectivity and sensitivity of antibody-based DNA-m6A methods, revealing most commercial antibodies as poorly selective towards DNA-m6A. Finally, using a validated highly selective anti-DNA-m6A antibody we expose distinct patterns of DNA-m6A in C. reinhardtii, A. thaliana, and D. melanogaster.

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Antibody

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Published
March, 2022

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Products used in this publication

  • Mouse IgG
    C15200082-50
    N6-methyladenosine (m6A) monoclonal antibody

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  • 64th ASH Annual Meeting and Exposition 2022
    New Orleans, Louisiana, USA
    Dec 10-Dec 13, 2022
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