Diagenode

LSD1 polyclonal antibody (sample size)

Catalog Number
Format
Price
C15410067-10
10 µl
$115.00
  Bulk order
Other format



Alternative names: BHC110, AOF2, EC1, KDM1

Polyclonal antibody raised in rabbit against human LSD1 (Lysine-specific demethylase 1), using a KLH-conjugated synthetic peptide from the inner part of the protein.

LotA57-00234P
Concentration2.2 µg/µl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP /ChIP-seq * 1 μg/ChIP Fig 1, 2
ELISA 1:1,000 - 1:10,000 Fig 3
Western Blotting 1:4,000 Fig 4
Immunofluorescence 1:200 Fig 5

* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation data

    LSD1 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1
    ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the “iDeal ChIP-seq” kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    LSD1 Antibody ChIP-seq Grade

    LSD1 Antibody for ChIP-seq

    LSD1 Antibody for ChIP-seq assay

    LSD1 Antibody for ChIP-seq assay

    LSD1 Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1
    ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 μg of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.

    LSD1 Antibody ELISA validation

    Figure 3. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.

    LSD1 Antibody validated in Western Blot

    Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1
    Western blot was performed using nuclear extracts from HeLa cells (40 μg) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.

    LSD1 Antibody validated in Western Blot

    Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1
    Whole cell extracts (40 μg) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    LSD1 Antibody validated in Immunofluorescence

    Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1
    HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  • Target Description

    LSD1 (lysine specific demethylase 1, UniProt/Swiss-Prot entry O60341) is a component of the histone demethylase complex that uses FAD as a prosthetic goup. LSD1 may have a dual effect on gene transcription. As it demethylates the mono- and dimethylated ‘Lys-4’ of histone H3, which are associated with transcriptional activation, LSD1 can act as a repressor of gene expression. However, LSD1 is also capable of demethylating ‘Lys-9’ of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to activation of androgen receptor target genes. LSD1 therefore mediates different processes such as embryonic development, cell differentiation and proliferation, stem and cancer cell biology

  •  実験手法
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
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    siRNA Knockdown
    Epigenetic antibodies you can trust! Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level o... Read more
  •  資料
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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    Datasheet LSD1 C15410067 DATASHEET
    Datasheet description
    Download
  •  Safety sheets
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: LSD1 polyclonal antibody (sample size) (Diagenode Cat# C15410067-10 Lot# A57-00234P). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Interactome Networks of FOSL1 and FOSL2 in Human Th17 Cells.
    Shetty Ankitha et al.
    Dysregulated function of Th17 cells has implications in immunodeficiencies and autoimmune disorders. Th17 cell differentiation is orchestrated by a complex network of transcription factors, including several members of the activator protein (AP-1) family. Among the latter, FOSL1 and FOSL2 modulate the effector funct...

    HOTAIR lncRNA promotes epithelial-mesenchymal transition byredistributing LSD1 at regulatory chromatin regions.
    Jarroux, J. et al.
    Epithelial-to-mesenchymal transition (EMT) describes the loss of epithelial traits and gain of mesenchymal traits by normal cells during development and by neoplastic cells during cancer metastasis. The long noncoding RNA HOTAIR triggers EMT, in part by serving as a scaffold for PRC2 and thus promoting repressive hi...

    LSD1 regulates the balance between self-renewal and differentiation in human embryonic stem cells.
    Adamo A, Sesé B, Boue S, Castaño J, Paramonov I, Barrero MJ, Izpisua Belmonte JC
    We identify LSD1 (lysine-specific demethylase 1; also known as KDM1A and AOF2) as a key histone modifier that participates in the maintenance of pluripotency through the regulation of bivalent domains, a chromatin environment present at the regulatory regions of developmental genes that contains both H3K4 di/trimeth...

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