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IGF2BP1 polyclonal antibody

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Catalog Number

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C15410349

100 µg

$380.00
Bulk order

Other names: CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1

Polyclonal antibody raised in rabbit against human IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.

Lot	001
Concentration	1 μg/μl
Species reactivity	Human: positive. Other species: not tested.
Type	Polyclonal
Purity	Affinity purified polyclonal antibody.
Host	Rabbit
Storage Conditions	Store at -20°C.
Storage Buffer	PBS containing 50% glycerol, does not contain a preservative.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
RIP	15 µg per 10⁷ cells	Fig 1
Western blotting	1:1,000	Fig 2
IP	5 µg per 2.5x10⁶ cells	Fig 3

Validation data

Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1
Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 µg of the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.

Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1
Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1
Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 µg of the Diagenode monoclonal antibody against IGF2BP1 (Cat. No. C15410349, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP1 protein was subsequently detected by western blot with the IGF2BP1 antibody as described above.

資料
IGF2BP1 polyclonal antibody datasheet
Polyclonal antibody raised in rabbit against human IGF2BP1 (Insulin Like Growth Factor 2 mRNA Bin... Download

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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 &micro;g of the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1</strong><br />Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (<span>Cat</span>. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 &micro;g of the Diagenode monoclonal antibody against IGF2BP1 (<span>Cat</span>. No. C15410349, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP1 protein was subsequently detected by western blot with the IGF2BP1 antibody as described above.</small></p>
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			'created' => '2015-07-17 10:12:18',
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$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
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	(int) 1 => (int) 1895
)
$chipseq_service = array(
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	(int) 1 => (int) 1835,
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	(int) 3 => (int) 2684,
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	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '30',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'IP',
	'description' => '<p>Immunoprecipitation</p>',
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	'online' => true,
	'tabular' => true,
	'slug' => 'immunoprecipitation',
	'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
	'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 12:23:07',
	'created' => '2015-07-08 13:46:50',
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		'application_id' => '30'
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	'id' => '30',
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	'parent_id' => '40',
	'name' => 'IP',
	'description' => '<p>Immunoprecipitation</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'immunoprecipitation',
	'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
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	'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 12:23:07',
	'created' => '2015-07-08 13:46:50',
	'locale' => 'jpn'
)
$description = '<p>Immunoprecipitation</p>'
$name = 'IP'
$document = array(
	'id' => '1018',
	'name' => 'IGF2BP1 polyclonal antibody datasheet',
	'description' => '<p>Polyclonal antibody raised in rabbit against human IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>',
	'image_id' => null,
	'type' => '',
	'url' => '../files/products/antibodies/igf2bp1-polyclonal-antibody-100-ug.pdf',
	'slug' => 'igf2bp1-polyclonal-antibody-100-ug-tds',
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	'meta_description' => '',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'jp',
	'meta_keywords' => '',
	'meta_description' => '',
	'meta_title' => 'IGF2BP1 polyclonal antibody',
	'product' => array(
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			'id' => '2976',
			'antibody_id' => '532',
			'name' => 'IGF2BP1 polyclonal antibody',
			'description' => '<p><strong>Other names:</strong> CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1</p><p>Polyclonal antibody raised in rabbit against human IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>',
			'label1' => 'Validation data',
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 &micro;g of the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1</strong><br />Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (<span>Cat</span>. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 &micro;g of the Diagenode monoclonal antibody against IGF2BP1 (<span>Cat</span>. No. C15410349, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP1 protein was subsequently detected by western blot with the IGF2BP1 antibody as described above.</small></p>
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<thead>
<tr>
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<th>References</th>
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<tr>
<td>RIP</td>
<td>15 &micro;g per 10<sup>7</sup> cells</td>
<td>Fig 1</td>
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<tr>
<td>Western blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
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<tr>
<td>IP</td>
<td>5 &micro;g per 2.5x10<sup>6</sup> cells</td>
<td>Fig 3</td>
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		'name' => 'IGF2BP1 polyclonal antibody',
		'description' => '<p><strong>Other names:</strong> CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1</p><p>Polyclonal antibody raised in rabbit against human IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>',
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		'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 &micro;g of the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1</strong><br />Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (<span>Cat</span>. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 &micro;g of the Diagenode monoclonal antibody against IGF2BP1 (<span>Cat</span>. No. C15410349, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP1 protein was subsequently detected by western blot with the IGF2BP1 antibody as described above.</small></p>
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		'clonality' => '',
		'isotype' => '',
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<th>References</th>
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<td>RIP</td>
<td>15 &micro;g per 10<sup>7</sup> cells</td>
<td>Fig 1</td>
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<tr>
<td>Western blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
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<tr>
<td>IP</td>
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<td>Fig 3</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<li>100% satisfaction guarantee</li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1</strong><br />Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (<span>Cat</span>. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 &micro;g of the Diagenode monoclonal antibody against IGF2BP1 (<span>Cat</span>. No. C15410349, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP1 protein was subsequently detected by western blot with the IGF2BP1 antibody as described above.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1</strong><br />Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (<span>Cat</span>. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 &micro;g of the Diagenode monoclonal antibody against IGF2BP1 (<span>Cat</span>. No. C15410349, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP1 protein was subsequently detected by western blot with the IGF2BP1 antibody as described above.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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			'name' => 'IGF2BP1 polyclonal antibody',
			'description' => '<p><strong>Other names:</strong> CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1</p><p>Polyclonal antibody raised in rabbit against human IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410349-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 &micro;g of the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1</strong><br />Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (<span>Cat</span>. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 &micro;g of the Diagenode monoclonal antibody against IGF2BP1 (<span>Cat</span>. No. C15410349, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP1 protein was subsequently detected by western blot with the IGF2BP1 antibody as described above.</small></p>
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<thead>
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<td>Fig 1</td>
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<tr>
<td>Western blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
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<tr>
<td>IP</td>
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		'description' => '<p><strong>Other names:</strong> CRD-BP, VICKZ1, IMP-1, CRDBP, ZBP-1, IMP1, ZBP1</p><p>Polyclonal antibody raised in rabbit against human IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>',
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million K562 cells using 15 &micro;g of the Diagenode antibody against IGF2BP1 (Cat. No. C15410349) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the IGF2BP1 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the IGF2BP1 antibody and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against IGF2BP1</strong><br />Whole cell extracts from K562, 293T, HeLa and Jurkat cells (lanes 1, 2, 3 and 4, respectively) were analysed by Western blot using the Diagenode antibody against IGF2BP1 (<span>Cat</span>. No. C15410349) diluted 1:1,000 in TBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against IGF2BP1</strong><br />Immunoprecipitation was performed on whole cell extracts from K562 cells using 5 &micro;g of the Diagenode monoclonal antibody against IGF2BP1 (<span>Cat</span>. No. C15410349, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated IGF2BP1 protein was subsequently detected by western blot with the IGF2BP1 antibody as described above.</small></p>
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		'created' => '2018-06-28 14:36:19',
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		'id' => '532',
		'name' => 'IGF2BP1 polyclonal antibody',
		'description' => 'IGF2BP1 (UniProtKB/Swiss-Prot entry Q9NZI8) is a member of the insulin-like growth factor 2 mRNA-binding protein family. It binds to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, thereby regulating translation.',
		'clonality' => '',
		'isotype' => '',
		'lot' => '001',
		'concentration' => '1 μg/μl',
		'reactivity' => 'Human: positive. Other species: not tested.',
		'type' => 'Polyclonal',
		'purity' => 'Affinity purified polyclonal antibody.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>RIP</td>
<td>15 &micro;g per 10<sup>7</sup> cells</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
</tr>
<tr>
<td>IP</td>
<td>5 &micro;g per 2.5x10<sup>6</sup> cells</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>',
		'storage_conditions' => 'Store at -20°C.',
		'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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		'slug' => 'igf2bp1-polyclonal-antibody',
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		'meta_description' => '',
		'modified' => '2018-06-27 15:23:01',
		'created' => '2018-06-27 15:23:01',
		'select_label' => '532 - IGF2BP1 polyclonal antibody (001 - 1 μg/μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)'
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			'modified' => '2016-01-21 08:09:31',
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
			'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
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			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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<div style="text-align: justify;" class="large-12 columns">
<p><span style="font-weight: 400;">Diagenode&rsquo;s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA-associated proteins studies below.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>
</div>
</div>
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			'meta_description' => 'Diagenode offers Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing',
			'meta_title' => 'Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing | Diagenode',
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			'description' => '<p>Polyclonal antibody raised in rabbit against human IGF2BP1 (Insulin Like Growth Factor 2 mRNA Binding Protein 1), using KLH-conjugated synthetic peptide from the C-teminal part of the protein.</p>',
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			'url' => '../files/products/antibodies/igf2bp1-polyclonal-antibody-100-ug.pdf',
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			'modified' => '2018-07-11 15:57:39',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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