FOXA2 polyclonal antibody

Catalog Number
50 μg
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Other names: HNF3B, TFC3B, HNF-3B, TCF-3B

Polyclonal antibody raised in rabbit against human FOXA2 (Forkhead Box A2), using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.

Concentration2.4 μg/μl
Species reactivityHuman: positive. Other species: not tested.
PurityAffinity purified polyclonal antibody in PBS containing 0.05% azide.
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 2 μg/ChIP Fig 1, 2
ELISA 1:5,000 Fig 3
WB 1:500 Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation data

    FOXA2 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2
    ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    FOXA2 Antibody ChIP-seq Grade

    FOXA2 Antibody for ChIP-seq

    FOXA2 Antibody for ChIP-seq assay

    FOXA2 Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2
    ChIP was performed with 2 μg of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).

    FOXA2 Antibody ELISA Validation

    Figure 3. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.

    FOXA2 Antibody validated in Western Blot

    Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2
    Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

  • Target Description

    FOXA2 (UniProtKB/Swiss-Prot entry Q9Y261) is a member of the forkhead class of transcription factors which are involved in the transcriptional activation of liver-specific genes such as AFP, albumin, tyrosine aminotransferase, PEPCK and transthyretin. FOXA2 also plays a role glucose homeostasis and in the regulation of fat metabolism and is required for the embryonic development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs. The FOXA2 gene has been linked to sporadic cases of maturity-onset diabetes of the young.

  •  実験手法
    ChIP-seq (ab)
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    ChIP-qPCR (ab)
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    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  資料
    FOXA2 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human FOXA2 (Forkhead Box A2), using two synthetic p...
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: FOXA2 polyclonal antibody (Diagenode Cat# C15410343 Lot# A2683-0040). Click here to copy to clipboard.

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