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ER alpha monoclonal antibody - Classic

50 µg/25 µl
  Bulk order

Alternative names:ESR, ESR1, ESRA, NR3A1

Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32).

Concentration2.0 µg/µl
Species reactivityHuman
PurityAmmonium sulphate purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 5 μg/ChIP Fig 1
Western Blotting 7 μg/ml Fig 2
Immunochemistry 15 μg/ml Fig 3
* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.
  • Validation Data


    Figure 1. ChIP using the Diagenode monoclonal antibody
    ChIP using the Diagenode monoclonal antibody against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.

    Western Blot

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα
    Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.


    Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα
    COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.

  •  実験手法
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
    Immunochemistry Read more
  •  資料
    Datasheet hERalpha MAb-009-050 DATASHEET
    Datasheet description
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: ER alpha monoclonal antibody - Classic (Diagenode Cat# C15200009 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer
    Adurthi S. et al.
    Oestrogen controls Foxp3 expression in regulatory T cells (Treg cells) via a mechanism thought to involve oestrogen receptor alpha (ERα), but the molecular basis and functional impact of ERα signalling in Treg cells remain unclear. We report that ERα ligand oestradiol (E2) is significantly increase...

    An oestrogen-receptor-alpha-bound human chromatin interactome.
    Fullwood MJ, Liu MH, Pan YF, Liu J, Xu H, Mohamed YB, Orlov YL, Velkov S, Ho A, Mei PH, Chew EG, Huang PY, Welboren WJ, Han Y, Ooi HS, Ariyaratne PN, Vega VB, Luo Y, Tan PY, Choy PY, Wansa KD, Zhao B, Lim KS, Leow SC, Yow JS, Joseph R, Li H, Desai KV, Tho
    Genomes are organized into high-level three-dimensional structures, and DNA elements separated by long genomic distances can in principle interact functionally. Many transcription factors bind to regulatory DNA elements distant from gene promoters. Although distal binding sites have been shown to regulate transcript...

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