Local euchromatin enrichment in lamina-associated domains anticipatestheir repositioning in the adipogenic lineage.

Madsen-Østerbye J. et al.

BACKGROUND: Interactions of chromatin with the nuclear lamina via lamina-associated domains (LADs) confer structural stability to the genome. The dynamics of positioning of LADs during differentiation, and how LADs impinge on developmental gene expression, remains, however, elusive. RESULTS: We examined changes in the association of lamin B1 with the genome in the first 72 h of differentiation of adipose stem cells into adipocytes. We demonstrate a repositioning of entire stand-alone LADs and of LAD edges as a prominent nuclear structural feature of early adipogenesis. Whereas adipogenic genes are released from LADs, LADs sequester downregulated or repressed genes irrelevant for the adipose lineage. However, LAD repositioning only partly concurs with gene expression changes. Differentially expressed genes in LADs, including LADs conserved throughout differentiation, reside in local euchromatic and lamin-depleted sub-domains. In these sub-domains, pre-differentiation histone modification profiles correlate with the LAD versus inter-LAD outcome of these genes during adipogenic commitment. Lastly, we link differentially expressed genes in LADs to short-range enhancers which overall co-partition with these genes in LADs versus inter-LADs during differentiation. CONCLUSIONS: We conclude that LADs are predictable structural features of adipose nuclear architecture that restrain non-adipogenic genes in a repressive environment.

Microplex Library Preparation kit

Share this article

April, 2022


Products used in this publication

  • ChIP-seq Grade
    H3K27ac Antibody - ChIP-seq Grade
  • ChIP-seq Grade
    H3K4me1 Antibody - ChIP-seq Grade
  • cut and tag antibody icon
    H3K4me3 Antibody - ChIP-seq Grade
  • cut and tag antibody icon
    H3K4me3 Antibody - ChIP-seq Grade
  • ChIP kit icon
    MicroPlex Library Preparation Kit v2 (12 indexes)
  • chip kit icon
    MicroPlex Library Preparation Kit v3 /48 rxns


  • EpiPlant 2024
    Clermont-Ferrand, France
    Jul 10-Jul 12, 2024


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy