Diagenode

Radiation-induced H3K9 methylation on E-cadherin promoter mediated byROS/Snail axis : Role of G9a signaling during lung epithelial-mesenchymaltransition.


Nagaraja, SunilGowda Sunnaghatta and Subramanian, Umadevi and Nagarajan,Devipriya

Lung cancer patients who have undergone radiotherapy developed severe complications such as pneumonitis and fibrosis. Upon irradiation, epithelial cells acquire mesenchymal phenotype via a process called epithelial to mesenchymal transition (EMT), which plays a vital role in organ fibrosis. Several mechanisms have been studied on EMT, however, the correlation between radiation-induced EMT and epigenetic changes are not well known. In the present study, we investigated the role of histone methyltransferase G9a on radiation-induced EMT signaling. There was an increase in total global histone methylation level in irradiated epithelial cells. Western blot analysis on irradiated cells showed an increased expression of H3K9me2/3. The pre-treatment of G9a inhibitor enhanced E-cadherin expression and decreased the mesenchymal markers like N-cadherin, vimentin in the radiated group. Surprisingly, radiation-induced ROS generation and pERK1/2 levels were also inhibited by G9a inhibitor BIX01294, which is showing its antioxidant potential. The ChIP-qPCR analysis on the E-cadherin promoter suggested that G9a and Snail might have formed complex to enrich suppressive marker H3K9me2/3. On the whole, our present study suggested that 1] ROS could modify H3K9 methylation via G9a and promote radiation-induced lung EMT in Beas2B and A549 cells 2] E-cadherin promoter enrichment with heterochromatin mark H3K9me2 expression upon irradiation could be modified by regulating G9a methyltransferase.

Tags
iDeal ChIP-seq Kit for Histones

Share this article

Published
October, 2020

Source

Products used in this publication

  • ChIP kit icon
    C01010051
    iDeal ChIP-seq kit for Histones

活动

  • IHEC Annual meeting 2022
    Quebec, Canada
    Oct 4-Oct 7, 2022
  • Danish RNA Society's 7th Annual Meeting on RNA
    Aarhus, Denmark
    Oct 11, 2022
  • ASHG 2022
    Los Angeles, California, USA
    Oct 25-Oct 29, 2022
  • International Conference on Epigenetics and Bioengineering 2022
    Houston, Texas, USA
    Oct 27-Oct 29, 2022
  • Long-Read Sequencing Meeting Uppsala LRUA22 Meeting
    Uppsala, Sweden
    Oct 31-Nov 2, 2022
  • Association for Molecular Pathology 2022
    Phoenix, Arizona, USA
    Nov 1-Nov 5, 2022
 查看所有活动

新闻

 查看所有新闻


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics