Protocols

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• Download Mass-Spectrometry: procedure for shotgun proteomics
  Mass Spectrometry is the preferred method for the sequencing and characterization of proteins. Shotgun proteomics analysis of cell lines and tissues relies on stringent isolation of proteins in add...
• Download Re-shearing of decrosslinked immunoprecipitated DNA using the Bioruptor® Pico
  ChIP-seq is a common tool used to study protein-DNA interactions in cells and tissues on a genome-wide scale. The size of the immunoprecipitated DNA fragments is a key parameter for successful libr...
• Download Chromatin shearing with the Bioruptor® Pico, 0.2 ml Bioruptor® Microtubes and the corresponding holder
  The first critical step of a successful ChIP experiment is the preparation of sheared chromatin with a suitable fragment range which best represents the biological scenario being investigated. The ...
• Download DNA shearing for Next-Generation Sequencing with the Bioruptor® Pico (2013-2019), 0.2 ml Bioruptor® microtubes and the corresponding holder
  Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, among others the precise DNA fragmentation. The Diagenode Bioruptor® Pico ensures that the DNA is e...
• Download CRISPR/Cas9 editing: mutation detection with mismatch cleavage assay
  Genome editing using CRISPR/Cas9 is used for targeted mutagenesis. But because genome editing does not target all loci with similar efficiencies, the mutation hit-rate at a given locus needs to be ...
• Download Transfection of CRISPR/Cas9 Nuclease NLS ribonucleoprotein (RNP) into adherent mammalian cells using Lipofectamine® RNAiMAX
  The CRISPR/Cas genome editing system consists of a single guide RNA (sgRNA) and the Cas9 endonuclease. To create gene disruptions, a single guide RNA is generated to direct the Cas9 nuclease to a s...
• Download Workflow for FFPE-derived DNA shearing prior to Next Generation Sequencing
  Formalin fixation and paraffin embedding (FFPE) is a standard method for long-term preservation of most archived pathological specimens. Archival FFPE samples provide an invaluable repository of in...
• Download iST Sample Preparation with the Bioruptor®
  The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico enhances mass spectrometric analyses.
• Download Ultrasensitive proteomics for microdissected fresh frozen tissue samples using the Bioruptor® Pico for sample preparation
  Histology-defined proteomics investigations are a fundamental tool in modern molecular pathology. Molecular signatures of specific cells must be detected within their correct histopathological cont...
• Download DNA shearing guide
  DNA shearing for Next-Generation Sequencing with the Bioruptor Pico