D-Plex cell-free RNA-seq Service

D-Plex cell-free RNA-seq is our innovative NGS technology for capturing the most comprehensive profile of circulating RNA from low-input liquid biopsy samples such as plasma and saliva, expanding the possibilities beyond miRNA alone. This service, leveraging the D-Plex cell-free RNA-seq kit (link when available), enables the analysis of all cfRNA biotypes present in liquid biopsies, including fragmented protein-coding transcripts (mRNA), long non-coding RNA (lncRNA), pseudogenes, and small non-coding RNA (sncRNA). This coverage is substantially broader than miRNA-focused approaches and extends to tRNA, piRNA, snRNA, and other species, with enhanced detection powered by our published method MGcount [1].

This method represents a new frontier for cfRNA biomarker discovery in plasma, going beyond the historically dominant focus on miRNA alone. Leverage our exclusive D-Plex-based service to gain deeper insights from your liquid biopsy studies.

The Most Comprehensive Low-Input Circulating cfRNA Profiling

• Higher reproducibility across operators and robust performance even at lowest input
• Designed for challenging liquid biopsy samples (plasma, saliva, etc.)
• Compatible with low input volumes, down to 0.2 mL of plasma
• End-to-end service, from plasma cfRNA extraction to comprehensive RNA biotype analysis and biomarker discovery
• Multi-omics integration with cfDNA methylation (Human Methylome) and Histone’s PTM form circulating nucleosomes (Nu.Q Discover)

[1] Hita et al., “MGcount: a total RNA-seq quantification tool to address multi-mapping and multi-overlapping alignments ambiguity in non-coding transcripts” BMC Bioinformatics. 2022 Jan 14;23:39