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<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
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<p></p>
<p></p>
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<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
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<p><span size="1" style="font-size: xx-small;">Representation of elution model. Elution changment may occur for different experimental models. Performed on HeLa cell culture meidium. </span></p>
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<h2 style="text-align: center;"><strong><img src="https://www.diagenode.com/img/categories/library-prep/flux-exosomes.png" alt="" usemap="#fluxmap" width="811" height="334" /></strong></h2>
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<p><span style="font-weight: 400;"></span></p>
<p><span style="font-weight: 400;"></span></p>
<p><span style="font-weight: 400;">The identification of reliable predictive or diagnostic biomarkers is crucial for biomedical research. Recent evidence shows that <strong>exosomes</strong>, small vesicles that are excreted into the extracellular environment, are excellent disease biomarker candidates as they contain specific biological material including <strong>small non-coding RNAs</strong> (microRNAs and others) and proteins involved in intercellular communication.</span></p>
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<p><span style="font-weight: 400;"></span></p>
<h3 class="red"><strong>Benefits of our optimal exosome capture solutions </strong></h3>
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<li><span style="font-weight: 400;">Fully compatible with our <strong>D-Plex</strong> technology (<a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">D-Plex Small RNA-seq kit</a>)</span></li>
<li><span style="font-weight: 400;"><strong>High yields</strong> from exosome pre-enrichment </span></li>
<li><strong>Global</strong> and <strong>specific</strong> exosome isolation options</li>
<li><strong>Guaranteed results</strong> with high level of validation</li>
<li><strong>User-friendlly</strong> method - no ultracentrifugation needed</li>
<li>Broad applications - RNA and microRNA (miRNA) NGS analysis, protein analysis, Western blot, ELISA, flow cytometry</li>
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<h3></h3>
<div class="row" id="workflow"> <img src="https://www.diagenode.com/img/product/kits/exosome-workflow-all.png" alt="" usemap="#wfmap" width="500" height="871" /></div>
<h3 class="red"><strong>microRNAs highlight</strong><strong>ed after exosomes isolation</strong></h3>
<p>MicroRNA differential expression analysis was performed on RNA-seq data from RNA isolated from exosomes captured from HEK293T cell culture supernatant and total RNA isolated from HEK293T cells.</p>
<p>The RNA libraries were prepared using Diagenode's <a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">RNA-seq library preparation</a> protocol for small RNA analysis. Reference genomes were obtained from the UCSC genome browser.</p>
<p><img src="https://www.diagenode.com/img/categories/exosome/micro-rnas-highlighted.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p>We observed several upregulated and downregulated microRNAs between exosomes isolated from cell culture supernatant and total HEK293T cells.</p>
<p style="padding-left: 30px;">- microRNA upregulation including miR7704, mirR4532, miR6087, miR4497, miR3687, miR19a-3p</p>
<p style="padding-left: 30px;">- microRNA downregulation including miR663a, miR4454, miR222-3p, miR196b-5p, miR10a-5p</p>
<p><map name="fluxmap">
<area shape="rect" coords="1610,865,1985,985" href="../p/CATS-Small-RNA-seq-Kit" alt="CATS Small RNA seq-Kit" />
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<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
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<p><span style="font-weight: 400;">The identification of reliable predictive or diagnostic biomarkers is crucial for biomedical research. Recent evidence shows that <strong>exosomes</strong>, small vesicles that are excreted into the extracellular environment, are excellent disease biomarker candidates as they contain specific biological material including <strong>small non-coding RNAs</strong> (microRNAs and others) and proteins involved in intercellular communication.</span></p>
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<li><span style="font-weight: 400;"><strong>High yields</strong> from exosome pre-enrichment </span></li>
<li><strong>Global</strong> and <strong>specific</strong> exosome isolation options</li>
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<div class="row" id="workflow"> <img src="https://www.diagenode.com/img/product/kits/exosome-workflow-all.png" alt="" usemap="#wfmap" width="500" height="871" /></div>
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<p>MicroRNA differential expression analysis was performed on RNA-seq data from RNA isolated from exosomes captured from HEK293T cell culture supernatant and total RNA isolated from HEK293T cells.</p>
<p>The RNA libraries were prepared using Diagenode's <a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">RNA-seq library preparation</a> protocol for small RNA analysis. Reference genomes were obtained from the UCSC genome browser.</p>
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<p style="padding-left: 30px;">- microRNA upregulation including miR7704, mirR4532, miR6087, miR4497, miR3687, miR19a-3p</p>
<p style="padding-left: 30px;">- microRNA downregulation including miR663a, miR4454, miR222-3p, miR196b-5p, miR10a-5p</p>
<p><map name="fluxmap">
<area shape="rect" coords="1610,865,1985,985" href="../p/CATS-Small-RNA-seq-Kit" alt="CATS Small RNA seq-Kit" />
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<h2 style="text-align: center;"><strong><img src="https://www.diagenode.com/img/categories/library-prep/flux-exosomes.png" alt="" usemap="#fluxmap" width="811" height="334" /></strong></h2>
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<p><span style="font-weight: 400;">The identification of reliable predictive or diagnostic biomarkers is crucial for biomedical research. Recent evidence shows that <strong>exosomes</strong>, small vesicles that are excreted into the extracellular environment, are excellent disease biomarker candidates as they contain specific biological material including <strong>small non-coding RNAs</strong> (microRNAs and others) and proteins involved in intercellular communication.</span></p>
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<h3 class="red"><strong>Benefits of our optimal exosome capture solutions </strong></h3>
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<li><span style="font-weight: 400;">Fully compatible with our <strong>D-Plex</strong> technology (<a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">D-Plex Small RNA-seq kit</a>)</span></li>
<li><span style="font-weight: 400;"><strong>High yields</strong> from exosome pre-enrichment </span></li>
<li><strong>Global</strong> and <strong>specific</strong> exosome isolation options</li>
<li><strong>Guaranteed results</strong> with high level of validation</li>
<li><strong>User-friendlly</strong> method - no ultracentrifugation needed</li>
<li>Broad applications - RNA and microRNA (miRNA) NGS analysis, protein analysis, Western blot, ELISA, flow cytometry</li>
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<div class="row" id="workflow"> <img src="https://www.diagenode.com/img/product/kits/exosome-workflow-all.png" alt="" usemap="#wfmap" width="500" height="871" /></div>
<h3 class="red"><strong>microRNAs highlight</strong><strong>ed after exosomes isolation</strong></h3>
<p>MicroRNA differential expression analysis was performed on RNA-seq data from RNA isolated from exosomes captured from HEK293T cell culture supernatant and total RNA isolated from HEK293T cells.</p>
<p>The RNA libraries were prepared using Diagenode's <a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">RNA-seq library preparation</a> protocol for small RNA analysis. Reference genomes were obtained from the UCSC genome browser.</p>
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<p>We observed several upregulated and downregulated microRNAs between exosomes isolated from cell culture supernatant and total HEK293T cells.</p>
<p style="padding-left: 30px;">- microRNA upregulation including miR7704, mirR4532, miR6087, miR4497, miR3687, miR19a-3p</p>
<p style="padding-left: 30px;">- microRNA downregulation including miR663a, miR4454, miR222-3p, miR196b-5p, miR10a-5p</p>
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<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
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<p><span size="1" style="font-size: xx-small;">Representation of elution model. Elution changment may occur for different experimental models. Performed on HeLa cell culture meidium. </span></p>
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<h2 style="text-align: center;"><strong><img src="https://www.diagenode.com/img/categories/library-prep/flux-exosomes.png" alt="" usemap="#fluxmap" width="811" height="334" /></strong></h2>
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<p><span style="font-weight: 400;"></span></p>
<p><span style="font-weight: 400;">The identification of reliable predictive or diagnostic biomarkers is crucial for biomedical research. Recent evidence shows that <strong>exosomes</strong>, small vesicles that are excreted into the extracellular environment, are excellent disease biomarker candidates as they contain specific biological material including <strong>small non-coding RNAs</strong> (microRNAs and others) and proteins involved in intercellular communication.</span></p>
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<h3 class="red"><strong>Benefits of our optimal exosome capture solutions </strong></h3>
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<li><span style="font-weight: 400;">Fully compatible with our <strong>D-Plex</strong> technology (<a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">D-Plex Small RNA-seq kit</a>)</span></li>
<li><span style="font-weight: 400;"><strong>High yields</strong> from exosome pre-enrichment </span></li>
<li><strong>Global</strong> and <strong>specific</strong> exosome isolation options</li>
<li><strong>Guaranteed results</strong> with high level of validation</li>
<li><strong>User-friendlly</strong> method - no ultracentrifugation needed</li>
<li>Broad applications - RNA and microRNA (miRNA) NGS analysis, protein analysis, Western blot, ELISA, flow cytometry</li>
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<h3></h3>
<div class="row" id="workflow"> <img src="https://www.diagenode.com/img/product/kits/exosome-workflow-all.png" alt="" usemap="#wfmap" width="500" height="871" /></div>
<h3 class="red"><strong>microRNAs highlight</strong><strong>ed after exosomes isolation</strong></h3>
<p>MicroRNA differential expression analysis was performed on RNA-seq data from RNA isolated from exosomes captured from HEK293T cell culture supernatant and total RNA isolated from HEK293T cells.</p>
<p>The RNA libraries were prepared using Diagenode's <a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">RNA-seq library preparation</a> protocol for small RNA analysis. Reference genomes were obtained from the UCSC genome browser.</p>
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<p>We observed several upregulated and downregulated microRNAs between exosomes isolated from cell culture supernatant and total HEK293T cells.</p>
<p style="padding-left: 30px;">- microRNA upregulation including miR7704, mirR4532, miR6087, miR4497, miR3687, miR19a-3p</p>
<p style="padding-left: 30px;">- microRNA downregulation including miR663a, miR4454, miR222-3p, miR196b-5p, miR10a-5p</p>
<p><map name="fluxmap">
<area shape="rect" coords="1610,865,1985,985" href="../p/CATS-Small-RNA-seq-Kit" alt="CATS Small RNA seq-Kit" />
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<area shape="rect" coords="940,1180,1110,1245" href="../p/exoip-composite-kit" alt="ExoIP Composite Kit" />
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<h6>Advantages of CaptEV :</h6>
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<p></p>
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<ul>
<li>
<h6>Validation by electron microscopy </h6>
</li>
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<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
<p></p>
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<ul>
<li>
<h6>Validation by Flow Cytometry</h6>
</li>
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<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
<p></p>
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