Diagenode

CaptEV cell culture

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目录号
格式
价格
C28030001-10
10 ml
$265.00
其他格式

CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.

  • Exosomes

    Exosomes are small extracellular vesicules of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.

    Exosomes contain various molecular constituents of their cell of origin, including proteins and microRNA. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.

    Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.

  • Product description

    Description

    The CaptEV reagent has been designed to concentrate and precipitate all extracellular vesicles(EVs) within serum or plasma blood samples to avoid bias. After precipitation, the exosome-rich suspension can be further purified by:

    • immunocapture with Diagenode’s ExoIP™ immunocapture kits for a selective isolation
    • EVCleaner Diagenode Column for a global isolation

    Advantages of CaptEV :

    • Full recovery of EVs
    • Enhanced immunocapture
    • Better signal coverage in FACS than with other reagents
    • Ease of use
    • Reproducibility
    • Intact and preserved exosomes (not sticky)

    Validation Data

    We used several methods to charachterize and validate exosome isolated with both CaptEV and ExoIP™ immunocapture kit:

    • Validation by electron microscopy 

    TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. 

    • Validation by Flow Cytometry

    Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). 

  •  文档
    CaptEV reagent MANUAL
    CaptEV: EV pre-enrichment reagent
    Download
  •  出版物

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