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<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a style="color: #13b29c; background-color: transparent; display: inline; padding: 0;" href="#more">Read more</a>
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<p style="text-align: justify;">CATS relies on polynucleotide tailing for capturing the 3’-ends of nucleic acids and the template switching ability of MMLV-RT to capture the 5’ ends for cDNA synthesis. This kit allows for generating Illumina compatible ready-to-sequence libraries from <strong>RNA inputs as low as 100 picograms</strong> of rRNA depleted material in just few hours.</p>
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<h3>More transcripts detected</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/rRNA-total-RNA-seq-1ng-10ng.png" /></div>
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<p>Biotypes* represented in the libraries <strong>CATS v2 total RNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2. <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000). * < 2% of total detected transcripts at this threshold is rRNA</p>
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<h3>Very reproducible library preparation method</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_4_R1_scatter_replicates_CATS.png" /></div>
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<p>Transcripts detected in two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
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<div>
<h3>High correlation in coding transcripts detection across different inputs</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_7_R_scatter_inputs_CATS.png" /></div>
<div class="large-5 small-12 medium-5 columns">
<p>Transcripts detected in 10 ng vs 1 ng samples of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
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<div>
<h3>Few PCR cycles, less amplification bias</h3>
<div class="large-12 small-12 medium-12 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-Fig_1ng-rRNAdplt-RNA-CATSV2.png" /></div>
<div class="large-12 small-12 medium-12 columns">
<p>BioAnalyzer® electropherogram of two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
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<div>
<h3>CATS saves time</h3>
<img src="https://www.diagenode.com/img/categories/library-prep/cats-save-time.png" /></div>
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<ul>
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<li>Excellent reproducibility with ultra low inputs down to 100 pg</li>
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</ul>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a style="color: #13b29c; background-color: transparent; display: inline; padding: 0;" href="#more">Read more</a>
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<p style="text-align: justify;">CATS relies on polynucleotide tailing for capturing the 3’-ends of nucleic acids and the template switching ability of MMLV-RT to capture the 5’ ends for cDNA synthesis. This kit allows for generating Illumina compatible ready-to-sequence libraries from <strong>RNA inputs as low as 100 picograms</strong> of rRNA depleted material in just few hours.</p>
<p style="text-align: justify;">Libraries retain <strong>strand specificity</strong> of origin. Unlike competing solutions, our CATS RNA-seq kit exhibits <strong>higher library efficiency</strong> versus ligation-based methods providing <strong>higher complexity</strong> from better template capture and minimal bias due to <strong>lower amplification</strong> requirements.</p>
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<h3>More transcripts detected</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/rRNA-total-RNA-seq-1ng-10ng.png" /></div>
<div class="large-5 small-12 medium-5 columns">
<p>Biotypes* represented in the libraries <strong>CATS v2 total RNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2. <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000). * < 2% of total detected transcripts at this threshold is rRNA</p>
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<h3>Very reproducible library preparation method</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_4_R1_scatter_replicates_CATS.png" /></div>
<div class="large-5 small-12 medium-5 columns">
<p>Transcripts detected in two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
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<div>
<h3>High correlation in coding transcripts detection across different inputs</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_7_R_scatter_inputs_CATS.png" /></div>
<div class="large-5 small-12 medium-5 columns">
<p>Transcripts detected in 10 ng vs 1 ng samples of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
</div>
</div>
<div>
<h3>Few PCR cycles, less amplification bias</h3>
<div class="large-12 small-12 medium-12 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-Fig_1ng-rRNAdplt-RNA-CATSV2.png" /></div>
<div class="large-12 small-12 medium-12 columns">
<p>BioAnalyzer® electropherogram of two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
</div>
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<div>
<h3>CATS saves time</h3>
<img src="https://www.diagenode.com/img/categories/library-prep/cats-save-time.png" /></div>
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<h4><span>Discover our<span> </span><strong>NEW<a href="https://www.diagenode.com/en/p/D-Plex-Total-RNA-seq-Library-Prep-x24"><span> </span>D-Plex Total RNA-seq Kit for Illumina</a></strong><a href="https://www.diagenode.com/en/p/D-Plex-Total-RNA-seq-Library-Prep-x24">.</a></span></h4>',
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'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;">
<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li>
<li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li>
<li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li>
<li><span style="font-weight: 400;">Enjoy a fast, easy, single tube protocol</span></li>
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<p></p>
<p></p>
<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Total RNA-seq Library Preparation Kit is a tool designed for the study of the whole coding and non-coding transcriptome. <span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span> </span>D-Plex technology</a><span><span> </span>to generate directional libraries for Illumina sequencing directly from total RNAs, mRNAs that has already been enriched by poly(A) selection, or RNAs that has already been depleted of rRNAs.</span></p>
<p><span>The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p>
<p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. <span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.</span></span></p>
<p><span><span>D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
</ul>
<div class="extra-spaced" align="center"></div>
<div class="row">
<div class="carrousel" style="background-position: center;">
<div class="slick">
<div>
<h3>Greater sensitivity to detect novel transcripts</h3>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p>
</div>
</div>
<div>
<h3>Highest diversity to facilitate biomarker discovery</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p>
</div>
</div>
<div>
<h3>Consistent expression profiling across a wide range of RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p>
</div>
</div>
<div>
<h3>Superior library complexity at low RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div>
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<p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p>
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<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
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<p><span style="font-weight: 400;">Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to </span><a href="../applications/dna-rna-shearing"><span style="font-weight: 400;">fragmented DNA or RNA</span></a><span style="font-weight: 400;"> prior to sequencing</span></p>
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<center><img src="https://www.diagenode.com/img/categories/library-prep/flux.png" /></center>
<p><span style="font-weight: 400;">Diagenode offers a comprehensive product portfolio for library preparation:<br /></span></p>
<strong><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex RNA-seq Library Preparation Kits</a></strong><br />
<p><span style="font-weight: 400;">Diagenode’s new RNA-sequencing solutions utilize the innovative c</span><span style="font-weight: 400;">apture and a</span><span style="font-weight: 400;">mplification by t</span><span style="font-weight: 400;">ailing and s</span><span style="font-weight: 400;">witching”</span><span style="font-weight: 400;">, a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of RNA. </span><span style="font-weight: 400;"></span><a href="../categories/Library-preparation-for-RNA-seq">Learn more</a></p>
<strong><a href="../categories/library-preparation-for-ChIP-seq">ChIP-seq and DNA sequencing library preparation solutions</a></strong><br />
<p><span style="font-weight: 400;">Our kits have been optimized for DNA library preparation used for next generation sequencing for a wide range of inputs. Using a simple three-step protocols, our</span><a href="http://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><span style="font-weight: 400;"> </span></a><span style="font-weight: 400;">kits are an optimal choice for library preparation from DNA inputs down to 50 pg. </span><a href="../categories/library-preparation-for-ChIP-seq">Learn more</a></p>
<a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span><strong>Bioruptor Pico - short fragments</strong></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br />
<p><span style="font-weight: 400;"></span><span style="font-weight: 400;">Our well-cited Bioruptor Pico is the shearing device of choice for chromatin and DNA fragmentation. Obtain uniform and tight fragment distributions between 150bp -2kb. </span><a href="../p/bioruptor-pico-sonication-device">Learn more</a></p>
<strong><a href="../p/megaruptor2-1-unit"><span href="../p/bioruptor-pico-sonication-device">Megaruptor</span>® - long fragments</a></strong><a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br />
<p><span style="font-weight: 400;"></span><span style="font-weight: 400;">The Megaruptor is designed to shear DNA from 3kb-75kb for long-read sequencing. <a href="../p/megaruptor2-1-unit">Learn more</a></span></p>
<span href="../p/bioruptor-pico-sonication-device"></span><span style="font-weight: 400;"></span></div>
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'description' => '<p>The salmon louse Lepeophtheirus salmonis is a marine ectoparasite that has a detrimental impact on salmon farms. Genomic knowledge of adult stages is critical to understand the reproductive success and lifecycle completion of this species. Here, we report a comprehensive characterization of the L. salmonis miRNome with emphasis on the sex-differences of the parasite. Small-RNA sequencing was conducted on males and females, and mRNA-sequencing was also conducted to identify miRNA-targets at these stages. Based on bioinformatics analyses, 3101 putative miRNAs were found in L. salmonis, including precursors and variants. The most abundant and over-expressed miRNAs belonged to the bantam, mir-100, mir-1, mir-263a and mir-276 families, while the most differentially expressed mRNAs corresponded to genes related to reproduction and other biological processes involved in cell-differentiation. Target analyses revealed that the most up-regulated miRNAs in males can act by inhibiting the expression of genes related to female differentiation such as vitellogenin genes. Target prediction and expression patterns suggested a pivotal role of miRNAs in the reproductive development of L. salmonis.</p>',
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'description' => '<p><span>Chemical signals are a key element of host-parasite interactions. In marine ecosystems, obligate ectoparasites, such as sea lice, use chemical cues and other sensory signals to increase the probability of encountering a host and to identify appropriate hosts on which they depend to complete their life cycle. The chemical compounds that underlie host identification by the sea lice are not fully described or characterized. Here, we report a novel compound - the Atlantic salmon (</span><i>Salmo salar</i><span>) antimicrobial peptide cathelicidin-2 (Cath-2) – that acts as an activation cue for the marine parasitic copepod<span> </span></span><i>Lepeophtheirus salmonis</i><span>.<span> </span></span><i>L</i><span>.<span> </span></span><i>salmonis</i><span><span> </span>were exposed to 0, 7, 70 and 700 ppb of Cath-2 and neural activity, swimming behaviour and gene expression profiles of animals in response to the peptide were evaluated. The neurophysiological, behavioural and transcriptomic results were consistent:<span> </span></span><i>L</i><span>.<span> </span></span><i>salmonis</i><span><span> </span>detects Cath-2 as a water-soluble peptide released from the skin of salmon, triggering chemosensory neural activity associated with altered swimming behaviour of copepodids exposed to the peptide, and chemosensory-related genes were up-regulated in copepodids exposed to the peptide.<span> </span></span><i>L</i><span>.<span> </span></span><i>salmonis</i><span><span> </span>are activated by Cath-2, indicating a tight link between this peptide and the salmon louse chemosensory system.</span></p>',
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'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/30213966',
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<h6 style="height:60px">D-Plex Total RNA-seq Kit for Illumina</h6>
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<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li>
<li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li>
<li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li>
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<p></p>
<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Total RNA-seq Library Preparation Kit is a tool designed for the study of the whole coding and non-coding transcriptome. <span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span> </span>D-Plex technology</a><span><span> </span>to generate directional libraries for Illumina sequencing directly from total RNAs, mRNAs that has already been enriched by poly(A) selection, or RNAs that has already been depleted of rRNAs.</span></p>
<p><span>The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p>
<p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. <span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.</span></span></p>
<p><span><span>D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
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<div class="slick">
<div>
<h3>Greater sensitivity to detect novel transcripts</h3>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p>
</div>
</div>
<div>
<h3>Highest diversity to facilitate biomarker discovery</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p>
</div>
</div>
<div>
<h3>Consistent expression profiling across a wide range of RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p>
</div>
</div>
<div>
<h3>Superior library complexity at low RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p>
</div>
</div>
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<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
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'description' => '<p><strong>An updated version of this kit using D-Plex technology is available for superior performance.</strong></p>
<h4><span>Discover our<span> </span><strong>NEW<a href="https://www.diagenode.com/en/p/D-Plex-Total-RNA-seq-Library-Prep-x24"><span> </span>D-Plex Total RNA-seq Kit for Illumina</a></strong><a href="https://www.diagenode.com/en/p/D-Plex-Total-RNA-seq-Library-Prep-x24">.</a></span></h4>',
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<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li>
<li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li>
<li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li>
<li><span style="font-weight: 400;">Enjoy a fast, easy, single tube protocol</span></li>
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<p></p>
<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Total RNA-seq Library Preparation Kit is a tool designed for the study of the whole coding and non-coding transcriptome. <span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span> </span>D-Plex technology</a><span><span> </span>to generate directional libraries for Illumina sequencing directly from total RNAs, mRNAs that has already been enriched by poly(A) selection, or RNAs that has already been depleted of rRNAs.</span></p>
<p><span>The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p>
<p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. <span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.</span></span></p>
<p><span><span>D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
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<div class="extra-spaced" align="center"></div>
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<div class="carrousel" style="background-position: center;">
<div class="slick">
<div>
<h3>Greater sensitivity to detect novel transcripts</h3>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p>
</div>
</div>
<div>
<h3>Highest diversity to facilitate biomarker discovery</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p>
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<div>
<h3>Consistent expression profiling across a wide range of RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p>
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<div>
<h3>Superior library complexity at low RNA inputs</h3>
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<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p>
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<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
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<p><span style="font-weight: 400;">Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to </span><a href="../applications/dna-rna-shearing"><span style="font-weight: 400;">fragmented DNA or RNA</span></a><span style="font-weight: 400;"> prior to sequencing</span></p>
<p><span style="font-weight: 400;">After input DNA has been fragmented, it is end-repaired and blunt-ended</span><span style="font-weight: 400;">. The next step is a A-tailing in which dAMP is added to the 3´ end of the blunt phosphorylated DNA fragments to prevent concatemerization and to allow the ligation of adaptors with complementary dT overhangs. In addition, barcoded adapters can be incorporated to facilitate multiplexing prior to or during amplification.</span></p>
<center><img src="https://www.diagenode.com/img/categories/library-prep/flux.png" /></center>
<p><span style="font-weight: 400;">Diagenode offers a comprehensive product portfolio for library preparation:<br /></span></p>
<strong><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex RNA-seq Library Preparation Kits</a></strong><br />
<p><span style="font-weight: 400;">Diagenode’s new RNA-sequencing solutions utilize the innovative c</span><span style="font-weight: 400;">apture and a</span><span style="font-weight: 400;">mplification by t</span><span style="font-weight: 400;">ailing and s</span><span style="font-weight: 400;">witching”</span><span style="font-weight: 400;">, a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of RNA. </span><span style="font-weight: 400;"></span><a href="../categories/Library-preparation-for-RNA-seq">Learn more</a></p>
<strong><a href="../categories/library-preparation-for-ChIP-seq">ChIP-seq and DNA sequencing library preparation solutions</a></strong><br />
<p><span style="font-weight: 400;">Our kits have been optimized for DNA library preparation used for next generation sequencing for a wide range of inputs. Using a simple three-step protocols, our</span><a href="http://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><span style="font-weight: 400;"> </span></a><span style="font-weight: 400;">kits are an optimal choice for library preparation from DNA inputs down to 50 pg. </span><a href="../categories/library-preparation-for-ChIP-seq">Learn more</a></p>
<a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span><strong>Bioruptor Pico - short fragments</strong></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br />
<p><span style="font-weight: 400;"></span><span style="font-weight: 400;">Our well-cited Bioruptor Pico is the shearing device of choice for chromatin and DNA fragmentation. Obtain uniform and tight fragment distributions between 150bp -2kb. </span><a href="../p/bioruptor-pico-sonication-device">Learn more</a></p>
<strong><a href="../p/megaruptor2-1-unit"><span href="../p/bioruptor-pico-sonication-device">Megaruptor</span>® - long fragments</a></strong><a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br />
<p><span style="font-weight: 400;"></span><span style="font-weight: 400;">The Megaruptor is designed to shear DNA from 3kb-75kb for long-read sequencing. <a href="../p/megaruptor2-1-unit">Learn more</a></span></p>
<span href="../p/bioruptor-pico-sonication-device"></span><span style="font-weight: 400;"></span></div>
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'description' => '<p>The salmon louse Lepeophtheirus salmonis is a marine ectoparasite that has a detrimental impact on salmon farms. Genomic knowledge of adult stages is critical to understand the reproductive success and lifecycle completion of this species. Here, we report a comprehensive characterization of the L. salmonis miRNome with emphasis on the sex-differences of the parasite. Small-RNA sequencing was conducted on males and females, and mRNA-sequencing was also conducted to identify miRNA-targets at these stages. Based on bioinformatics analyses, 3101 putative miRNAs were found in L. salmonis, including precursors and variants. The most abundant and over-expressed miRNAs belonged to the bantam, mir-100, mir-1, mir-263a and mir-276 families, while the most differentially expressed mRNAs corresponded to genes related to reproduction and other biological processes involved in cell-differentiation. Target analyses revealed that the most up-regulated miRNAs in males can act by inhibiting the expression of genes related to female differentiation such as vitellogenin genes. Target prediction and expression patterns suggested a pivotal role of miRNAs in the reproductive development of L. salmonis.</p>',
'date' => '2019-06-01',
'pmid' => 'http://www.pubmed.gov/30772247',
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'authors' => 'Gustavo Núñez-Acuña, Cristian Gallardo-Escárate, David M. Fields, Steven Shema, Anne Berit Skiftesvik, Ignacio Ormazábal & Howard I. Browman',
'description' => '<p><span>Chemical signals are a key element of host-parasite interactions. In marine ecosystems, obligate ectoparasites, such as sea lice, use chemical cues and other sensory signals to increase the probability of encountering a host and to identify appropriate hosts on which they depend to complete their life cycle. The chemical compounds that underlie host identification by the sea lice are not fully described or characterized. Here, we report a novel compound - the Atlantic salmon (</span><i>Salmo salar</i><span>) antimicrobial peptide cathelicidin-2 (Cath-2) – that acts as an activation cue for the marine parasitic copepod<span> </span></span><i>Lepeophtheirus salmonis</i><span>.<span> </span></span><i>L</i><span>.<span> </span></span><i>salmonis</i><span><span> </span>were exposed to 0, 7, 70 and 700 ppb of Cath-2 and neural activity, swimming behaviour and gene expression profiles of animals in response to the peptide were evaluated. The neurophysiological, behavioural and transcriptomic results were consistent:<span> </span></span><i>L</i><span>.<span> </span></span><i>salmonis</i><span><span> </span>detects Cath-2 as a water-soluble peptide released from the skin of salmon, triggering chemosensory neural activity associated with altered swimming behaviour of copepodids exposed to the peptide, and chemosensory-related genes were up-regulated in copepodids exposed to the peptide.<span> </span></span><i>L</i><span>.<span> </span></span><i>salmonis</i><span><span> </span>are activated by Cath-2, indicating a tight link between this peptide and the salmon louse chemosensory system.</span></p>',
'date' => '2018-09-13',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/30213966',
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<p>将 <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> D-Plex Total RNA-seq Kit for Illumina</strong> 添加至我的购物车。</p>
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<h6 style="height:60px">D-Plex Total RNA-seq Kit for Illumina</h6>
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<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li>
<li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li>
<li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li>
<li><span style="font-weight: 400;">Enjoy a fast, easy, single tube protocol</span></li>
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<p></p>
<p></p>
<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Total RNA-seq Library Preparation Kit is a tool designed for the study of the whole coding and non-coding transcriptome. <span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span> </span>D-Plex technology</a><span><span> </span>to generate directional libraries for Illumina sequencing directly from total RNAs, mRNAs that has already been enriched by poly(A) selection, or RNAs that has already been depleted of rRNAs.</span></p>
<p><span>The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p>
<p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. <span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.</span></span></p>
<p><span><span>D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
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<h3>Greater sensitivity to detect novel transcripts</h3>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div>
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<p></p>
<p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p>
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<div>
<h3>Highest diversity to facilitate biomarker discovery</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div>
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<p></p>
<p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p>
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<h3>Consistent expression profiling across a wide range of RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div>
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<p></p>
<p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p>
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<div>
<h3>Superior library complexity at low RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div>
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<p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p>
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<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
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'description' => '<p class="p1">The Diagenode D-Plex Total RNA-seq Library Preparation kit is a tool designed for the study of the whole coding and non-coding transcriptome. The present kit incorporates the unique D-Plex technology to generate directional RNA libraries for Illumina sequencing directly from total RNAs, messenger RNAs (mRNAs) that have already been enriched by poly(A) capture, or RNAs that have already been depleted of ribosomal RNAs (rRNAs).</p>',
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'description' => '<p><span>Chemical signals are a key element of host-parasite interactions. In marine ecosystems, obligate ectoparasites, such as sea lice, use chemical cues and other sensory signals to increase the probability of encountering a host and to identify appropriate hosts on which they depend to complete their life cycle. The chemical compounds that underlie host identification by the sea lice are not fully described or characterized. Here, we report a novel compound - the Atlantic salmon (</span><i>Salmo salar</i><span>) antimicrobial peptide cathelicidin-2 (Cath-2) – that acts as an activation cue for the marine parasitic copepod<span> </span></span><i>Lepeophtheirus salmonis</i><span>.<span> </span></span><i>L</i><span>.<span> </span></span><i>salmonis</i><span><span> </span>were exposed to 0, 7, 70 and 700 ppb of Cath-2 and neural activity, swimming behaviour and gene expression profiles of animals in response to the peptide were evaluated. The neurophysiological, behavioural and transcriptomic results were consistent:<span> </span></span><i>L</i><span>.<span> </span></span><i>salmonis</i><span><span> </span>detects Cath-2 as a water-soluble peptide released from the skin of salmon, triggering chemosensory neural activity associated with altered swimming behaviour of copepodids exposed to the peptide, and chemosensory-related genes were up-regulated in copepodids exposed to the peptide.<span> </span></span><i>L</i><span>.<span> </span></span><i>salmonis</i><span><span> </span>are activated by Cath-2, indicating a tight link between this peptide and the salmon louse chemosensory system.</span></p>',
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<p style="text-align: justify;">CATS relies on polynucleotide tailing for capturing the 3’-ends of nucleic acids and the template switching ability of MMLV-RT to capture the 5’ ends for cDNA synthesis. This kit allows for generating Illumina compatible ready-to-sequence libraries from <strong>RNA inputs as low as 100 picograms</strong> of rRNA depleted material in just few hours.</p>
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<h3>More transcripts detected</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/rRNA-total-RNA-seq-1ng-10ng.png" /></div>
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<p>Biotypes* represented in the libraries <strong>CATS v2 total RNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2. <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000). * < 2% of total detected transcripts at this threshold is rRNA</p>
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<h3>Very reproducible library preparation method</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_4_R1_scatter_replicates_CATS.png" /></div>
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<p>Transcripts detected in two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
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<h3>High correlation in coding transcripts detection across different inputs</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_7_R_scatter_inputs_CATS.png" /></div>
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<p>Transcripts detected in 10 ng vs 1 ng samples of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
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<h3>Few PCR cycles, less amplification bias</h3>
<div class="large-12 small-12 medium-12 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-Fig_1ng-rRNAdplt-RNA-CATSV2.png" /></div>
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<p>BioAnalyzer® electropherogram of two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
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<h3>CATS saves time</h3>
<img src="https://www.diagenode.com/img/categories/library-prep/cats-save-time.png" /></div>
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'description' => '<p style="text-align: justify;">Diagenode’s CATS total RNA-seq Kit (with rRNA depletion) utilizes the innovative “<strong>C</strong>apture and <strong>A</strong>mplification by <strong>T</strong>ailing and <strong>S</strong>witching” (CATS), a <strong>ligation-free method</strong> to produce DNA libraries for next generation sequencing from low input amounts of RNA. </p>
<ul>
<li>Diverse transcript detection</li>
<li>Excellent reproducibility with ultra low inputs down to 100 pg</li>
<li>Optimal performance with challenging samples</li>
</ul>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a style="color: #13b29c; background-color: transparent; display: inline; padding: 0;" href="#more">Read more</a>
<div class="content" id="more">
<p style="text-align: justify;">CATS relies on polynucleotide tailing for capturing the 3’-ends of nucleic acids and the template switching ability of MMLV-RT to capture the 5’ ends for cDNA synthesis. This kit allows for generating Illumina compatible ready-to-sequence libraries from <strong>RNA inputs as low as 100 picograms</strong> of rRNA depleted material in just few hours.</p>
<p style="text-align: justify;">Libraries retain <strong>strand specificity</strong> of origin. Unlike competing solutions, our CATS RNA-seq kit exhibits <strong>higher library efficiency</strong> versus ligation-based methods providing <strong>higher complexity</strong> from better template capture and minimal bias due to <strong>lower amplification</strong> requirements.</p>
</div>
</li>
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<div class="container">
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<div>
<h3>More transcripts detected</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/rRNA-total-RNA-seq-1ng-10ng.png" /></div>
<div class="large-5 small-12 medium-5 columns">
<p>Biotypes* represented in the libraries <strong>CATS v2 total RNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2. <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000). * < 2% of total detected transcripts at this threshold is rRNA</p>
</div>
</div>
<div>
<h3>Very reproducible library preparation method</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_4_R1_scatter_replicates_CATS.png" /></div>
<div class="large-5 small-12 medium-5 columns">
<p>Transcripts detected in two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
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<div>
<h3>High correlation in coding transcripts detection across different inputs</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_7_R_scatter_inputs_CATS.png" /></div>
<div class="large-5 small-12 medium-5 columns">
<p>Transcripts detected in 10 ng vs 1 ng samples of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
</div>
</div>
<div>
<h3>Few PCR cycles, less amplification bias</h3>
<div class="large-12 small-12 medium-12 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-Fig_1ng-rRNAdplt-RNA-CATSV2.png" /></div>
<div class="large-12 small-12 medium-12 columns">
<p>BioAnalyzer® electropherogram of two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
</div>
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<div>
<h3>CATS saves time</h3>
<img src="https://www.diagenode.com/img/categories/library-prep/cats-save-time.png" /></div>
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<h4><span>Discover our<span> </span><strong>NEW<a href="https://www.diagenode.com/en/p/D-Plex-Total-RNA-seq-Library-Prep-x24"><span> </span>D-Plex Total RNA-seq Kit for Illumina</a></strong><a href="https://www.diagenode.com/en/p/D-Plex-Total-RNA-seq-Library-Prep-x24">.</a></span></h4>',
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'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;">
<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li>
<li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li>
<li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li>
<li><span style="font-weight: 400;">Enjoy a fast, easy, single tube protocol</span></li>
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<p></p>
<p></p>
<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Total RNA-seq Library Preparation Kit is a tool designed for the study of the whole coding and non-coding transcriptome. <span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span> </span>D-Plex technology</a><span><span> </span>to generate directional libraries for Illumina sequencing directly from total RNAs, mRNAs that has already been enriched by poly(A) selection, or RNAs that has already been depleted of rRNAs.</span></p>
<p><span>The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p>
<p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. <span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.</span></span></p>
<p><span><span>D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
</ul>
<div class="extra-spaced" align="center"></div>
<div class="row">
<div class="carrousel" style="background-position: center;">
<div class="slick">
<div>
<h3>Greater sensitivity to detect novel transcripts</h3>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p>
</div>
</div>
<div>
<h3>Highest diversity to facilitate biomarker discovery</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p>
</div>
</div>
<div>
<h3>Consistent expression profiling across a wide range of RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p>
</div>
</div>
<div>
<h3>Superior library complexity at low RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p>
</div>
</div>
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<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
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<p><img src="https://www.diagenode.com/img/product/kits/dplex/bioinfoPipe.png" alt="Small RNA seq Bioinformatics pipeline" width="925" height="196" /></p>',
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<p><span style="font-weight: 400;">Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to </span><a href="../applications/dna-rna-shearing"><span style="font-weight: 400;">fragmented DNA or RNA</span></a><span style="font-weight: 400;"> prior to sequencing</span></p>
<p><span style="font-weight: 400;">After input DNA has been fragmented, it is end-repaired and blunt-ended</span><span style="font-weight: 400;">. The next step is a A-tailing in which dAMP is added to the 3´ end of the blunt phosphorylated DNA fragments to prevent concatemerization and to allow the ligation of adaptors with complementary dT overhangs. In addition, barcoded adapters can be incorporated to facilitate multiplexing prior to or during amplification.</span></p>
<center><img src="https://www.diagenode.com/img/categories/library-prep/flux.png" /></center>
<p><span style="font-weight: 400;">Diagenode offers a comprehensive product portfolio for library preparation:<br /></span></p>
<strong><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex RNA-seq Library Preparation Kits</a></strong><br />
<p><span style="font-weight: 400;">Diagenode’s new RNA-sequencing solutions utilize the innovative c</span><span style="font-weight: 400;">apture and a</span><span style="font-weight: 400;">mplification by t</span><span style="font-weight: 400;">ailing and s</span><span style="font-weight: 400;">witching”</span><span style="font-weight: 400;">, a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of RNA. </span><span style="font-weight: 400;"></span><a href="../categories/Library-preparation-for-RNA-seq">Learn more</a></p>
<strong><a href="../categories/library-preparation-for-ChIP-seq">ChIP-seq and DNA sequencing library preparation solutions</a></strong><br />
<p><span style="font-weight: 400;">Our kits have been optimized for DNA library preparation used for next generation sequencing for a wide range of inputs. Using a simple three-step protocols, our</span><a href="http://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><span style="font-weight: 400;"> </span></a><span style="font-weight: 400;">kits are an optimal choice for library preparation from DNA inputs down to 50 pg. </span><a href="../categories/library-preparation-for-ChIP-seq">Learn more</a></p>
<a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span><strong>Bioruptor Pico - short fragments</strong></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br />
<p><span style="font-weight: 400;"></span><span style="font-weight: 400;">Our well-cited Bioruptor Pico is the shearing device of choice for chromatin and DNA fragmentation. Obtain uniform and tight fragment distributions between 150bp -2kb. </span><a href="../p/bioruptor-pico-sonication-device">Learn more</a></p>
<strong><a href="../p/megaruptor2-1-unit"><span href="../p/bioruptor-pico-sonication-device">Megaruptor</span>® - long fragments</a></strong><a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br />
<p><span style="font-weight: 400;"></span><span style="font-weight: 400;">The Megaruptor is designed to shear DNA from 3kb-75kb for long-read sequencing. <a href="../p/megaruptor2-1-unit">Learn more</a></span></p>
<span href="../p/bioruptor-pico-sonication-device"></span><span style="font-weight: 400;"></span></div>
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<h6 style="height:60px">D-Plex Total RNA-seq Kit for Illumina</h6>
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<h2>Product Features</h2>
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<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
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<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
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<p>D-Plex Total RNA-seq Library Preparation Kit is a tool designed for the study of the whole coding and non-coding transcriptome. <span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span> </span>D-Plex technology</a><span><span> </span>to generate directional libraries for Illumina sequencing directly from total RNAs, mRNAs that has already been enriched by poly(A) selection, or RNAs that has already been depleted of rRNAs.</span></p>
<p><span>The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p>
<p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. <span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.</span></span></p>
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<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
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<p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p>
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<li class="accordion-navigation"><a style="color: #13b29c; background-color: transparent; display: inline; padding: 0;" href="#more">Read more</a>
<div class="content" id="more">
<p style="text-align: justify;">CATS relies on polynucleotide tailing for capturing the 3’-ends of nucleic acids and the template switching ability of MMLV-RT to capture the 5’ ends for cDNA synthesis. This kit allows for generating Illumina compatible ready-to-sequence libraries from <strong>RNA inputs as low as 100 picograms</strong> of rRNA depleted material in just few hours.</p>
<p style="text-align: justify;">Libraries retain <strong>strand specificity</strong> of origin. Unlike competing solutions, our CATS RNA-seq kit exhibits <strong>higher library efficiency</strong> versus ligation-based methods providing <strong>higher complexity</strong> from better template capture and minimal bias due to <strong>lower amplification</strong> requirements.</p>
</div>
</li>
</ul>
<div class="carrousel" style="background-position: center;">
<div class="container">
<div class="row" style="background: rgba(255,255,255,0.1); height: 241px;">
<div class="large-12 columns slick">
<div>
<h3>More transcripts detected</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/rRNA-total-RNA-seq-1ng-10ng.png" /></div>
<div class="large-5 small-12 medium-5 columns">
<p>Biotypes* represented in the libraries <strong>CATS v2 total RNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2. <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000). * < 2% of total detected transcripts at this threshold is rRNA</p>
</div>
</div>
<div>
<h3>Very reproducible library preparation method</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_4_R1_scatter_replicates_CATS.png" /></div>
<div class="large-5 small-12 medium-5 columns">
<p>Transcripts detected in two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
</div>
</div>
<div>
<h3>High correlation in coding transcripts detection across different inputs</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_7_R_scatter_inputs_CATS.png" /></div>
<div class="large-5 small-12 medium-5 columns">
<p>Transcripts detected in 10 ng vs 1 ng samples of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
</div>
</div>
<div>
<h3>Few PCR cycles, less amplification bias</h3>
<div class="large-12 small-12 medium-12 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-Fig_1ng-rRNAdplt-RNA-CATSV2.png" /></div>
<div class="large-12 small-12 medium-12 columns">
<p>BioAnalyzer® electropherogram of two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
</div>
</div>
<div>
<h3>CATS saves time</h3>
<img src="https://www.diagenode.com/img/categories/library-prep/cats-save-time.png" /></div>
</div>
</div>
</div>
</div>
<p><br /><br /></p>
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<ul>
<li>Diverse transcript detection</li>
<li>Excellent reproducibility with ultra low inputs down to 100 pg</li>
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<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a style="color: #13b29c; background-color: transparent; display: inline; padding: 0;" href="#more">Read more</a>
<div class="content" id="more">
<p style="text-align: justify;">CATS relies on polynucleotide tailing for capturing the 3’-ends of nucleic acids and the template switching ability of MMLV-RT to capture the 5’ ends for cDNA synthesis. This kit allows for generating Illumina compatible ready-to-sequence libraries from <strong>RNA inputs as low as 100 picograms</strong> of rRNA depleted material in just few hours.</p>
<p style="text-align: justify;">Libraries retain <strong>strand specificity</strong> of origin. Unlike competing solutions, our CATS RNA-seq kit exhibits <strong>higher library efficiency</strong> versus ligation-based methods providing <strong>higher complexity</strong> from better template capture and minimal bias due to <strong>lower amplification</strong> requirements.</p>
</div>
</li>
</ul>
<div class="carrousel" style="background-position: center;">
<div class="container">
<div class="row" style="background: rgba(255,255,255,0.1); height: 241px;">
<div class="large-12 columns slick">
<div>
<h3>More transcripts detected</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/rRNA-total-RNA-seq-1ng-10ng.png" /></div>
<div class="large-5 small-12 medium-5 columns">
<p>Biotypes* represented in the libraries <strong>CATS v2 total RNA-seq Kit</strong> vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2. <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000). * < 2% of total detected transcripts at this threshold is rRNA</p>
</div>
</div>
<div>
<h3>Very reproducible library preparation method</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_4_R1_scatter_replicates_CATS.png" /></div>
<div class="large-5 small-12 medium-5 columns">
<p>Transcripts detected in two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
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<h3>High correlation in coding transcripts detection across different inputs</h3>
<div class="large-7 small-12 medium-7 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-FIG_7_R_scatter_inputs_CATS.png" /></div>
<div class="large-5 small-12 medium-5 columns">
<p>Transcripts detected in 10 ng vs 1 ng samples of <strong>CATS v2 total RNA-seq libraries.</strong> TPM≥2 <br />Input: <strong>1 ng, 10 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
</div>
</div>
<div>
<h3>Few PCR cycles, less amplification bias</h3>
<div class="large-12 small-12 medium-12 columns"><img src="https://www.diagenode.com/img/product/kits/cats/NEW-Fig_1ng-rRNAdplt-RNA-CATSV2.png" /></div>
<div class="large-12 small-12 medium-12 columns">
<p>BioAnalyzer® electropherogram of two technical replicates of <strong>CATS v2 total RNA-seq libraries.</strong> <br />Input: <strong>1 ng</strong> rRNA(-) RNA from human universal total RNA (Agilent, 740000).</p>
</div>
</div>
<div>
<h3>CATS saves time</h3>
<img src="https://www.diagenode.com/img/categories/library-prep/cats-save-time.png" /></div>
</div>
</div>
</div>
</div>
<p><br /><br /></p>
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'description' => '<p><strong>An updated version of this kit using D-Plex technology is available for superior performance.</strong></p>
<h4><span>Discover our<span> </span><strong>NEW<a href="https://www.diagenode.com/en/p/D-Plex-Total-RNA-seq-Library-Prep-x24"><span> </span>D-Plex Total RNA-seq Kit for Illumina</a></strong><a href="https://www.diagenode.com/en/p/D-Plex-Total-RNA-seq-Library-Prep-x24">.</a></span></h4>',
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'description' => '<div class="small-12 medium-12 large-12 columns" style="border: 3px solid #B02736; padding: 10px; margin: 10px;">
<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li>
<li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li>
<li><span>Get high sensitivity data even from difficult samples, such as degraded, FFPE samples</span></li>
<li><span style="font-weight: 400;">Enjoy a fast, easy, single tube protocol</span></li>
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<p></p>
<p></p>
<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Total RNA-seq Library Preparation Kit is a tool designed for the study of the whole coding and non-coding transcriptome. <span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span> </span>D-Plex technology</a><span><span> </span>to generate directional libraries for Illumina sequencing directly from total RNAs, mRNAs that has already been enriched by poly(A) selection, or RNAs that has already been depleted of rRNAs.</span></p>
<p><span>The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p>
<p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. <span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.</span></span></p>
<p><span><span>D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
</ul>
<div class="extra-spaced" align="center"></div>
<div class="row">
<div class="carrousel" style="background-position: center;">
<div class="slick">
<div>
<h3>Greater sensitivity to detect novel transcripts</h3>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p>
</div>
</div>
<div>
<h3>Highest diversity to facilitate biomarker discovery</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p>
</div>
</div>
<div>
<h3>Consistent expression profiling across a wide range of RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p>
</div>
</div>
<div>
<h3>Superior library complexity at low RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p>
</div>
</div>
</div>
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<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
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<p><span style="font-weight: 400;">Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to </span><a href="../applications/dna-rna-shearing"><span style="font-weight: 400;">fragmented DNA or RNA</span></a><span style="font-weight: 400;"> prior to sequencing</span></p>
<p><span style="font-weight: 400;">After input DNA has been fragmented, it is end-repaired and blunt-ended</span><span style="font-weight: 400;">. The next step is a A-tailing in which dAMP is added to the 3´ end of the blunt phosphorylated DNA fragments to prevent concatemerization and to allow the ligation of adaptors with complementary dT overhangs. In addition, barcoded adapters can be incorporated to facilitate multiplexing prior to or during amplification.</span></p>
<center><img src="https://www.diagenode.com/img/categories/library-prep/flux.png" /></center>
<p><span style="font-weight: 400;">Diagenode offers a comprehensive product portfolio for library preparation:<br /></span></p>
<strong><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex RNA-seq Library Preparation Kits</a></strong><br />
<p><span style="font-weight: 400;">Diagenode’s new RNA-sequencing solutions utilize the innovative c</span><span style="font-weight: 400;">apture and a</span><span style="font-weight: 400;">mplification by t</span><span style="font-weight: 400;">ailing and s</span><span style="font-weight: 400;">witching”</span><span style="font-weight: 400;">, a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of RNA. </span><span style="font-weight: 400;"></span><a href="../categories/Library-preparation-for-RNA-seq">Learn more</a></p>
<strong><a href="../categories/library-preparation-for-ChIP-seq">ChIP-seq and DNA sequencing library preparation solutions</a></strong><br />
<p><span style="font-weight: 400;">Our kits have been optimized for DNA library preparation used for next generation sequencing for a wide range of inputs. Using a simple three-step protocols, our</span><a href="http://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><span style="font-weight: 400;"> </span></a><span style="font-weight: 400;">kits are an optimal choice for library preparation from DNA inputs down to 50 pg. </span><a href="../categories/library-preparation-for-ChIP-seq">Learn more</a></p>
<a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span><strong>Bioruptor Pico - short fragments</strong></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br />
<p><span style="font-weight: 400;"></span><span style="font-weight: 400;">Our well-cited Bioruptor Pico is the shearing device of choice for chromatin and DNA fragmentation. Obtain uniform and tight fragment distributions between 150bp -2kb. </span><a href="../p/bioruptor-pico-sonication-device">Learn more</a></p>
<strong><a href="../p/megaruptor2-1-unit"><span href="../p/bioruptor-pico-sonication-device">Megaruptor</span>® - long fragments</a></strong><a href="../p/bioruptor-pico-sonication-device"><span style="font-weight: 400;"></span></a><a href="../categories/library-preparation-for-ChIP-seq-and-DNA-sequencing"><span style="font-weight: 400;"></span></a><br />
<p><span style="font-weight: 400;"></span><span style="font-weight: 400;">The Megaruptor is designed to shear DNA from 3kb-75kb for long-read sequencing. <a href="../p/megaruptor2-1-unit">Learn more</a></span></p>
<span href="../p/bioruptor-pico-sonication-device"></span><span style="font-weight: 400;"></span></div>
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'name' => 'Characterization of the salmon louse Lepeophtheirus salmonis miRNome: Sex-biased differences related to the coding and non-coding RNA interplay.',
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'description' => '<p>The salmon louse Lepeophtheirus salmonis is a marine ectoparasite that has a detrimental impact on salmon farms. Genomic knowledge of adult stages is critical to understand the reproductive success and lifecycle completion of this species. Here, we report a comprehensive characterization of the L. salmonis miRNome with emphasis on the sex-differences of the parasite. Small-RNA sequencing was conducted on males and females, and mRNA-sequencing was also conducted to identify miRNA-targets at these stages. Based on bioinformatics analyses, 3101 putative miRNAs were found in L. salmonis, including precursors and variants. The most abundant and over-expressed miRNAs belonged to the bantam, mir-100, mir-1, mir-263a and mir-276 families, while the most differentially expressed mRNAs corresponded to genes related to reproduction and other biological processes involved in cell-differentiation. Target analyses revealed that the most up-regulated miRNAs in males can act by inhibiting the expression of genes related to female differentiation such as vitellogenin genes. Target prediction and expression patterns suggested a pivotal role of miRNAs in the reproductive development of L. salmonis.</p>',
'date' => '2019-06-01',
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'description' => '<p><span>Chemical signals are a key element of host-parasite interactions. In marine ecosystems, obligate ectoparasites, such as sea lice, use chemical cues and other sensory signals to increase the probability of encountering a host and to identify appropriate hosts on which they depend to complete their life cycle. The chemical compounds that underlie host identification by the sea lice are not fully described or characterized. Here, we report a novel compound - the Atlantic salmon (</span><i>Salmo salar</i><span>) antimicrobial peptide cathelicidin-2 (Cath-2) – that acts as an activation cue for the marine parasitic copepod<span> </span></span><i>Lepeophtheirus salmonis</i><span>.<span> </span></span><i>L</i><span>.<span> </span></span><i>salmonis</i><span><span> </span>were exposed to 0, 7, 70 and 700 ppb of Cath-2 and neural activity, swimming behaviour and gene expression profiles of animals in response to the peptide were evaluated. The neurophysiological, behavioural and transcriptomic results were consistent:<span> </span></span><i>L</i><span>.<span> </span></span><i>salmonis</i><span><span> </span>detects Cath-2 as a water-soluble peptide released from the skin of salmon, triggering chemosensory neural activity associated with altered swimming behaviour of copepodids exposed to the peptide, and chemosensory-related genes were up-regulated in copepodids exposed to the peptide.<span> </span></span><i>L</i><span>.<span> </span></span><i>salmonis</i><span><span> </span>are activated by Cath-2, indicating a tight link between this peptide and the salmon louse chemosensory system.</span></p>',
'date' => '2018-09-13',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/30213966',
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<h6 style="height:60px">D-Plex Total RNA-seq Kit for Illumina</h6>
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<h2>Product Features</h2>
<ul style="list-style-type: disc;">
<li><span style="font-weight: 400;">An innovative technology with template switching and UMIs</span></li>
<li><span style="font-weight: 400;">Ultra-low input capability, down to 50 pg for total RNAs</span></li>
<li><span style="font-weight: 400;">Capture the widest possible diversity of RNAs for rich content</span></li>
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<p></p>
<h4></h4>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/dplex-total-manual.pdf" target="_blank" title="dplex total rnaseq user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Total RNA-seq Library Preparation Kit is a tool designed for the study of the whole coding and non-coding transcriptome. <span>The kit is using the</span><a href="https://www.diagenode.com/en/pages/dplex" target="_blank"><span> </span>D-Plex technology</a><span><span> </span>to generate directional libraries for Illumina sequencing directly from total RNAs, mRNAs that has already been enriched by poly(A) selection, or RNAs that has already been depleted of rRNAs.</span></p>
<p><span>The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for comprehensive analysis of the transcriptome. It accurately measures gene and transcript abundance and captures the widest possible diversity of RNAs, including known and novel features in coding and non-coding RNAs.</span></p>
<p><span></span><span>D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. <span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.</span></span></p>
<p><span><span>D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:</span></span></p>
<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
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<h3>Greater sensitivity to detect novel transcripts</h3>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/CPM_totalrna.png" alt="total RNA kit" /></center></div>
<div class="large-12 small-12 medium-12 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq kit enables great transcript detection, even when starting from very low RNA inputs or working with challenging FFPE samples. Increased number of transcripts detected at 1x coverage is an indicator of greater sensitivity.</p>
</div>
</div>
<div>
<h3>Highest diversity to facilitate biomarker discovery</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_rdep_biotypes.png" alt="total RNA diversity" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>D-Plex Total RNA-seq libraries capture efficiently all RNA biotypes present in the sample of interest, both coding and non-coding RNAs or small and long RNAs.</p>
</div>
</div>
<div>
<h3>Consistent expression profiling across a wide range of RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/HUR_and_umeg_rdep_violin_tpm_10.png" alt="total RNA" width="450px" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Gene expression profiling (including protein coding exons and introns) obtained with D-Plex Total RNA-seq kit is conserved for decreasing RNA inputs, keeping transcript diversity across the range of RNA inputs.</p>
</div>
</div>
<div>
<h3>Superior library complexity at low RNA inputs</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><center><img src="https://www.diagenode.com/img/product/kits/dplex/robustness_corr_HUR_HuMEg_rrna.png" alt="total RNA" /></center></div>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns">
<p></p>
<p>Correlation analysis of the protein coding genes detected indicates superior transcript expression correlation between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for challenging samples such as FFPE preparations.</p>
</div>
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<ul>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-A" title="D-Plex UDI Module - Set A" target="_blank"><span>C05030021</span> - D-Plex Unique Dual Indexes for Illumina - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/D-Plex-24-Unique-Dual-Indexes-Set-B" title="D-Plex UDI Module - Set B" target="_blank"><span>C05030022</span> - D-Plex Unique Dual Indexes for Illumina - Set B</a></li>
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'meta_title' => 'D-Plex Total RNA-seq Library Prep Kit for Illumina | Diagenode ',
'meta_keywords' => 'RNA-seq kit, RNA-seq library preparation; low input RNA-seq, UMI RNA-seq, UDI RNA-seq, D-Plex, higher RNA sensitivity',
'meta_description' => 'RNA-seq library preparation for Illumina sequencing - Unique D-Plex technology - Optimized for ultra-low input (50 pg total RNA) - UMI reduce PCR biases - UDI detect index hopping - Compatibility with FFPE samples - User-friendly and fast protocol',
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'id' => '1134',
'name' => 'D-Plex Total RNA-seq Kit',
'description' => '<p class="p1">The Diagenode D-Plex Total RNA-seq Library Preparation kit is a tool designed for the study of the whole coding and non-coding transcriptome. The present kit incorporates the unique D-Plex technology to generate directional RNA libraries for Illumina sequencing directly from total RNAs, messenger RNAs (mRNAs) that have already been enriched by poly(A) capture, or RNAs that have already been depleted of ribosomal RNAs (rRNAs).</p>',
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'type' => 'Manual',
'url' => 'files/products/kits/dplex-total-rnaseq.pdf',
'slug' => 'dplex-total-rnaseq',
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'modified' => '2021-04-20 14:09:33',
'created' => '2021-04-20 14:09:33',
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'id' => '3386',
'name' => 'The Atlantic salmon (Salmo salar) antimicrobial peptide cathelicidin-2 is a molecular host-associated cue for the salmon louse (Lepeophtheirus salmonis)',
'authors' => 'Gustavo Núñez-Acuña, Cristian Gallardo-Escárate, David M. Fields, Steven Shema, Anne Berit Skiftesvik, Ignacio Ormazábal & Howard I. Browman',
'description' => '<p><span>Chemical signals are a key element of host-parasite interactions. In marine ecosystems, obligate ectoparasites, such as sea lice, use chemical cues and other sensory signals to increase the probability of encountering a host and to identify appropriate hosts on which they depend to complete their life cycle. The chemical compounds that underlie host identification by the sea lice are not fully described or characterized. Here, we report a novel compound - the Atlantic salmon (</span><i>Salmo salar</i><span>) antimicrobial peptide cathelicidin-2 (Cath-2) – that acts as an activation cue for the marine parasitic copepod<span> </span></span><i>Lepeophtheirus salmonis</i><span>.<span> </span></span><i>L</i><span>.<span> </span></span><i>salmonis</i><span><span> </span>were exposed to 0, 7, 70 and 700 ppb of Cath-2 and neural activity, swimming behaviour and gene expression profiles of animals in response to the peptide were evaluated. The neurophysiological, behavioural and transcriptomic results were consistent:<span> </span></span><i>L</i><span>.<span> </span></span><i>salmonis</i><span><span> </span>detects Cath-2 as a water-soluble peptide released from the skin of salmon, triggering chemosensory neural activity associated with altered swimming behaviour of copepodids exposed to the peptide, and chemosensory-related genes were up-regulated in copepodids exposed to the peptide.<span> </span></span><i>L</i><span>.<span> </span></span><i>salmonis</i><span><span> </span>are activated by Cath-2, indicating a tight link between this peptide and the salmon louse chemosensory system.</span></p>',
'date' => '2018-09-13',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/30213966',
'doi' => '',
'modified' => '2018-12-31 11:30:39',
'created' => '2018-09-20 11:15:54',
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
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