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Fast genomic muChIP-chip from 1,000 cells


Dahl JA, Reiner AH, Collas P.

Genome-wide location analysis of histone modifications and transcription factor binding relies on chromatin immunoprecipitation (ChIP) assays. These assays are, however, time-consuming and require large numbers of cells, hindering their application to the analysis of many interesting cell types. We report here a fast microChIP (μChIP) assay for 1,000 cells in combination with microarrays to produce genome-scale surveys of histone modifications. μChIP-chip reliably reproduces data obtained by large-scale assays: H3K9ac and H3K9m3 enrichment profiles are conserved and nucleosome-free regions are revealed.

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Products used in this publication

  • Antibody ChIP-seq grade icon
    C15410056
    H3K9me3 polyclonal antibody

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    Oct 15-Oct 19, 2019
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