Diagenode

PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex.


Link S, Spitzer RMM, Sana M, Torrado M, Völker-Albert MC, Keilhauer EC, Burgold T, Pünzeler S, Low JKK, Lindström I, Nist A, Regnard C, Stiewe T, Hendrich B, Imhof A, Mann M, Mackay JP, Bartkuhn M, Hake SB

Chromatin structure and function is regulated by reader proteins recognizing histone modifications and/or histone variants. We recently identified that PWWP2A tightly binds to H2A.Z-containing nucleosomes and is involved in mitotic progression and cranial-facial development. Here, using in vitro assays, we show that distinct domains of PWWP2A mediate binding to free linker DNA as well as H3K36me3 nucleosomes. In vivo, PWWP2A strongly recognizes H2A.Z-containing regulatory regions and weakly binds H3K36me3-containing gene bodies. Further, PWWP2A binds to an MTA1-specific subcomplex of the NuRD complex (M1HR), which consists solely of MTA1, HDAC1, and RBBP4/7, and excludes CHD, GATAD2 and MBD proteins. Depletion of PWWP2A leads to an increase of acetylation levels on H3K27 as well as H2A.Z, presumably by impaired chromatin recruitment of M1HR. Thus, this study identifies PWWP2A as a complex chromatin-binding protein that serves to direct the deacetylase complex M1HR to H2A.Z-containing chromatin, thereby promoting changes in histone acetylation levels.

Tags
Antibody
Microplex Library Preparation kit

Share this article

Published
October, 2018

Source

Products used in this publication

  • ChIP-seq Grade
    C15410202-50
    H2A.Zac polyclonal antibody
  • ChIP-seq Grade
    C15410069
    H3K27me3 polyclonal antibody
  • Mouse IgG
    C15310068
    H3K79me3 polyclonal antibody
  • ChIP-seq Grade
    C15410056
    H3K9me3 polyclonal antibody
  • ChIP kit icon
    C05010012
    MicroPlex Library Preparation Kit v2 (12 indices)

イベント

  • IHEC Annual meeting 2022
    Quebec, Canada
    Oct 4-Oct 7, 2022
  • Danish RNA Society's 7th Annual Meeting on RNA
    Aarhus, Denmark
    Oct 11, 2022
  • ASHG 2022
    Los Angeles, California, USA
    Oct 25-Oct 29, 2022
  • International Conference on Epigenetics and Bioengineering 2022
    Houston, Texas, USA
    Oct 27-Oct 29, 2022
  • Long-Read Sequencing Meeting Uppsala LRUA22 Meeting
    Uppsala, Sweden
    Oct 31-Nov 2, 2022
  • Association for Molecular Pathology 2022
    Phoenix, Arizona, USA
    Nov 1-Nov 5, 2022
 すべてのイベントを見る

ニュース

 すべてのニュースを見る


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics