Diagenode

Histone lysine trimethylation or acetylation can be modulated by phytoestrogen, estrogen or anti-HDAC in breast cancer cell lines.


Dagdemir A, Durif J, Ngollo M, Bignon YJ, Bernard-Gallon D

AIM: The isoflavones genistein, daidzein and equol (daidzein metabolite) have been reported to interact with epigenetic modifications, specifically hypermethylation of tumor suppressor genes. The objective of this study was to analyze and understand the mechanisms by which phytoestrogens act on chromatin in breast cancer cell lines. MATERIALS & METHODS: Two breast cancer cell lines, MCF-7 and MDA-MB 231, were treated with genistein (18.5 µM), daidzein (78.5 µM), equol (12.8 µM), 17β-estradiol (10 nM) and suberoylanilide hydroxamic acid (1 µM) for 48 h. A control with untreated cells was performed. 17β-estradiol and an anti-HDAC were used to compare their actions with phytoestrogens. The chromatin immunoprecipitation coupled with quantitative PCR was used to follow soy phytoestrogen effects on H3 and H4 histones on H3K27me3, H3K9me3, H3K4me3, H4K8ac and H3K4ac marks, and we selected six genes (EZH2, BRCA1, ERα, ERβ, SRC3 and P300) for analysis. RESULTS: Soy phytoestrogens induced a decrease in trimethylated marks and an increase in acetylating marks studied at six selected genes. CONCLUSION: We demonstrated that soy phytoestrogens tend to modify transcription through the demethylation and acetylation of histones in breast cancer cell lines.

Tags
Bioruptor
Chromatin Shearing
ChIP-qPCR
IP-Star
Antibody
H3K4me3 (C15410003)
HighCell ChIP kit
H3K27me3 (C15410069)
IP-Star Compact

Share this article

Published
February, 2013

Source

Products used in this publication

  • ChIP-seq Grade
    C15410039-50
    EZH2 polyclonal antibody
  • ChIP-seq Grade
    C15410069
    H3K27me3 polyclonal antibody
  • Antibody ChIP icon
    C15410165
    H3K4ac polyclonal antibody
  • cut and tag antibody icon
    C15410003-50
    H3K4me3 polyclonal antibody
  • ChIP-seq Grade
    C15410056
    H3K9me3 polyclonal antibody
  • Antibody ChIP icon
    C15410103
    H4K8ac polyclonal antibody
  • Mouse IgG
    C15410206
    Rabbit IgG
  • Compact Automated System
    B03000002
    SX-8G IP-Star® Compact Automated System

イベント

  • CLEPIC 2022
    Szczecin, Poland
    Jun 8-Jun 10, 2022
  • European Socienty of Human Genetics - ESHG 2022
    Wien, Austria
    Jun 11-Jun 14, 2022
  • Lausanne Genomics Days 2022
    Lausanne, Switzerland
    Jun 16-Jun 17, 2022
  • EpiPlant 2022
    Banyuls-Sur-Mer, France
    Jun 22-Jun 24, 2022
 すべてのイベントを見る

ニュース

 すべてのニュースを見る


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics