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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 μg/ChIP</td>
<td>Fig 1, 2</td>
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<tr>
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<td>1:1,000 - 1:3,000</td>
<td>Fig 3, 4, 5</td>
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<td>2 μg/IP</td>
<td>Fig 6</td>
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<td>1:100 - 1:1,000</td>
<td>Fig 7</td>
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<td>1:100 - 1:1,000</td>
<td>Fig 8</td>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p>
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<tr>
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<th>References</th>
</tr>
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<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1:3,000</td>
<td>Fig 3, 4, 5</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>2 μg/IP</td>
<td>Fig 6</td>
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<tr>
<td>Immunofluorescence</td>
<td>1:100 - 1:1,000</td>
<td>Fig 7</td>
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<tr>
<td>Immunohistochemistry</td>
<td>1:100 - 1:1,000</td>
<td>Fig 8</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p>
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</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p>
</div>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
</div>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p>
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<p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p>
<center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center>
<p></p>
<p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p>
<p>
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<p></p>
<p>
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'info3' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
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<tbody>
<tr valign="middle">
<td style="width: 213px;"></td>
<td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
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<p style="text-align: center;">< 0.1%</p>
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<td style="text-align: center; width: 180px;">
<p style="text-align: center;">0.2%</p>
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<td style="text-align: center; width: 154px;">
<p style="text-align: center;">1%</p>
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<td style="text-align: center; width: 155px;">
<p style="text-align: center;">0.5%</p>
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<td style="text-align: center; width: 180px;">
<p style="text-align: center;">Yes</p>
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<td style="text-align: center; width: 154px;">
<p style="text-align: center;">No</p>
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<td style="text-align: center; width: 155px;">
<p style="text-align: center;">Yes</p>
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<td style="width: 213px;">
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<td style="text-align: center; width: 180px;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center; width: 154px;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center; width: 155px;">
<p style="text-align: center;">up to 25 g of tissue</p>
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<tr style="background-color: #fff;" valign="middle">
<td style="width: 213px;">
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center; width: 208px;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center; width: 180px;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center; width: 154px;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center; width: 155px;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>TARDBP (TAR DNA binding protein),</strong> using a recombinant protein.</span></p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p>
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<p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p>
<center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center>
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<p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p>
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'info1' => '<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor/Bioruptor_pico_cooler_manual.pdf">Download</a></p>
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'label2' => 'Recommended settings for DNA shearing with Bioruptor® Pico',
'info2' => '<p>Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor® Pico</a></p>
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'label3' => 'Available chromatin shearing kits',
'info3' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table style="width: 925px;">
<tbody>
<tr valign="middle">
<td style="width: 213px;"></td>
<td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
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<p style="text-align: left;"><strong>SDS concentration</strong></p>
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<p style="text-align: center;">< 0.1%</p>
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<td style="text-align: center; width: 180px;">
<p style="text-align: center;">0.2%</p>
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<td style="text-align: center; width: 154px;">
<p style="text-align: center;">1%</p>
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<td style="text-align: center; width: 155px;">
<p style="text-align: center;">0.5%</p>
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<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
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<p style="text-align: center;">Yes</p>
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<td style="text-align: center; width: 180px;">
<p style="text-align: center;">Yes</p>
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<td style="text-align: center; width: 154px;">
<p style="text-align: center;">No</p>
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<td style="text-align: center; width: 155px;">
<p style="text-align: center;">Yes</p>
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<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center; width: 180px;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center; width: 154px;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center; width: 155px;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td style="width: 213px;">
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center; width: 208px;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center; width: 180px;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center; width: 154px;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center; width: 155px;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
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'antibody_id' => '427',
'name' => 'TARDBP Antibody',
'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>TARDBP (TAR DNA binding protein),</strong> using a recombinant protein.</span></p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p>
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</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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include - APP/View/Products/view.ctp, line 755
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View::render() - CORE/Cake/View/View.php, line 473
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ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p>
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<p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p>
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</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p>
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'info2' => '<p>TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.</p>',
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<p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p>
<center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center>
<p></p>
<p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p>
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'info3' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table style="width: 925px;">
<tbody>
<tr valign="middle">
<td style="width: 213px;"></td>
<td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
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<p style="text-align: center;">< 0.1%</p>
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<td style="text-align: center; width: 180px;">
<p style="text-align: center;">0.2%</p>
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<td style="text-align: center; width: 154px;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center; width: 155px;">
<p style="text-align: center;">0.5%</p>
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<td style="width: 213px;">
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<p style="text-align: center;">Yes</p>
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<td style="text-align: center; width: 180px;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center; width: 154px;">
<p style="text-align: center;">No</p>
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<td style="text-align: center; width: 155px;">
<p style="text-align: center;">Yes</p>
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<tr style="background-color: #fff;" valign="middle">
<td style="width: 213px;">
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<td style="text-align: center; width: 208px;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center; width: 180px;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center; width: 154px;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center; width: 155px;">
<p style="text-align: center;">up to 25 g of tissue</p>
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<tr style="background-color: #fff;" valign="middle">
<td style="width: 213px;">
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center; width: 208px;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center; width: 180px;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center; width: 154px;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center; width: 155px;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
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<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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'id' => '2384',
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'name' => 'TARDBP Antibody',
'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>TARDBP (TAR DNA binding protein),</strong> using a recombinant protein.</span></p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
</div>
</div>
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<p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p>
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<p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p>
<center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center>
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<td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
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<td style="text-align: center; width: 155px;">
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<p style="text-align: center;">Yes</p>
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<td style="text-align: center; width: 154px;">
<p style="text-align: center;">No</p>
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<td style="text-align: center; width: 155px;">
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<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
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<td style="text-align: center; width: 180px;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
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<td style="text-align: center; width: 154px;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
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<td style="text-align: center; width: 155px;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p>
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<p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p>
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'description' => '<p><span>Polyclonal antibody raised in rabbit against TARDBP (TAR DNA binding protein), using a recombinant protein.</span></p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p>
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<p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p>
</div>
</div>',
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'info2' => '<p>TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.</p>',
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'catalog_number' => 'C15410266-25',
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'sf_code' => 'C15410266-32380',
'type' => 'FRE',
'search_order' => '03-Antibody',
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'price_USD' => '150',
'price_GBP' => '115',
'price_JPY' => '18800',
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'price_AUD' => '375',
'country' => 'ALL',
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'slug' => 'tardbp-polyclonal-antibody-classic-25-mg',
'meta_title' => 'TARDBP polyclonal antibody - Classic',
'meta_keywords' => '',
'meta_description' => 'TARDBP polyclonal antibody - Classic',
'modified' => '2022-01-05 14:57:55',
'created' => '2015-06-29 14:08:20',
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'host' => '*****',
'id' => '427',
'name' => 'TARDBP polyclonal antibody - Classic',
'description' => 'TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.',
'clonality' => '',
'isotype' => '',
'lot' => '40135',
'concentration' => '1.01 μg/μl',
'reactivity' => 'Human, mouse, rat',
'type' => 'Polyclonal',
'purity' => 'Affinity purified',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1:3,000</td>
<td>Fig 3, 4, 5</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>2 μg/IP</td>
<td>Fig 6</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:100 - 1:1,000</td>
<td>Fig 7</td>
</tr>
<tr>
<td>Immunohistochemistry</td>
<td>1:100 - 1:1,000</td>
<td>Fig 8</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>',
'storage_conditions' => '',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
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'modified' => '2019-09-10 13:40:41',
'created' => '2015-07-28 14:11:25',
'select_label' => '427 - TARDBP polyclonal antibody - Classic (40135 - 1.01 μg/μl - Human, mouse, rat - Affinity purified - Rabbit)'
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'Group' => array(
'Group' => array(
'id' => '77',
'name' => 'C15410266',
'product_id' => '2384',
'modified' => '2016-02-18 21:46:45',
'created' => '2016-02-18 21:46:45'
),
'Master' => array(
'id' => '2384',
'antibody_id' => '427',
'name' => 'TARDBP Antibody',
'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>TARDBP (TAR DNA binding protein),</strong> using a recombinant protein.</span></p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p>
</div>
</div>',
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'info2' => '<p>TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.</p>',
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'meta_title' => 'TARDBP Antibody - ChIP-seq Grade (C15410266) | Diagenode',
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<p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p>
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<p></p>
<p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p>
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'label3' => 'Available chromatin shearing kits',
'info3' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table style="width: 925px;">
<tbody>
<tr valign="middle">
<td style="width: 213px;"></td>
<td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td style="width: 213px;">
<p style="text-align: left;"><strong>SDS concentration</strong></p>
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<td style="text-align: center; width: 208px;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center; width: 180px;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center; width: 154px;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center; width: 155px;">
<p style="text-align: center;">0.5%</p>
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<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
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<p style="text-align: center;">Yes</p>
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<td style="text-align: center; width: 180px;">
<p style="text-align: center;">Yes</p>
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<td style="text-align: center; width: 154px;">
<p style="text-align: center;">No</p>
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<td style="text-align: center; width: 155px;">
<p style="text-align: center;">Yes</p>
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<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
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<td style="text-align: center; width: 180px;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center; width: 154px;">
<p style="text-align: center;">100 million cells</p>
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<p style="text-align: center;">up to 25 g of tissue</p>
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<tr style="background-color: #fff;" valign="middle">
<td style="width: 213px;">
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center; width: 208px;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center; width: 180px;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center; width: 154px;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center; width: 155px;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p>
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'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p>
<p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p>
<center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center>
<p></p>
<p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p>
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'info1' => '<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor/Bioruptor_pico_cooler_manual.pdf">Download</a></p>
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'label3' => 'Available chromatin shearing kits',
'info3' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table style="width: 925px;">
<tbody>
<tr valign="middle">
<td style="width: 213px;"></td>
<td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td style="width: 213px;">
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center; width: 208px;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center; width: 180px;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center; width: 154px;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center; width: 155px;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td style="width: 213px;">
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center; width: 208px;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center; width: 180px;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center; width: 154px;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center; width: 155px;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td style="width: 213px;">
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center; width: 208px;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center; width: 180px;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center; width: 154px;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center; width: 155px;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td style="width: 213px;">
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center; width: 208px;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center; width: 180px;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center; width: 154px;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center; width: 155px;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p>
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<p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p>
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<p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p>
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'meta_description' => 'TARDBP polyclonal antibody - Classic',
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'description' => 'TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.',
'clonality' => '',
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'reactivity' => 'Human, mouse, rat',
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'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1:3,000</td>
<td>Fig 3, 4, 5</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>2 μg/IP</td>
<td>Fig 6</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:100 - 1:1,000</td>
<td>Fig 7</td>
</tr>
<tr>
<td>Immunohistochemistry</td>
<td>1:100 - 1:1,000</td>
<td>Fig 8</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>',
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'modified' => '2016-02-18 21:46:45',
'created' => '2016-02-18 21:46:45'
),
'Master' => array(
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'name' => 'TARDBP Antibody',
'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>TARDBP (TAR DNA binding protein),</strong> using a recombinant protein.</span></p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-7 columns">
<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.</p>',
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<p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p>
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<p></p>
<p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p>
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'info2' => '<p>Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor® Pico</a></p>
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'label3' => 'Available chromatin shearing kits',
'info3' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
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<tbody>
<tr valign="middle">
<td style="width: 213px;"></td>
<td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
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<p style="text-align: left;"><strong>SDS concentration</strong></p>
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<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center; width: 180px;">
<p style="text-align: center;">0.2%</p>
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<td style="text-align: center; width: 154px;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center; width: 155px;">
<p style="text-align: center;">0.5%</p>
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<p style="text-align: center;">Yes</p>
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<td style="text-align: center; width: 180px;">
<p style="text-align: center;">Yes</p>
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<td style="text-align: center; width: 154px;">
<p style="text-align: center;">No</p>
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<td style="text-align: center; width: 155px;">
<p style="text-align: center;">Yes</p>
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<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center; width: 180px;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center; width: 154px;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center; width: 155px;">
<p style="text-align: center;">up to 25 g of tissue</p>
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<tr style="background-color: #fff;" valign="middle">
<td style="width: 213px;">
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center; width: 208px;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center; width: 180px;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center; width: 154px;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center; width: 155px;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> Expert technical support</span></li>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p>
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'label3' => 'Available chromatin shearing kits',
'info3' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
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<tbody>
<tr valign="middle">
<td style="width: 213px;"></td>
<td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
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<p style="text-align: left;"><strong>SDS concentration</strong></p>
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<p style="text-align: center;">< 0.1%</p>
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<td style="text-align: center; width: 180px;">
<p style="text-align: center;">0.2%</p>
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<td style="text-align: center; width: 154px;">
<p style="text-align: center;">1%</p>
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<td style="text-align: center; width: 155px;">
<p style="text-align: center;">0.5%</p>
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<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
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<p style="text-align: center;">Yes</p>
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<td style="text-align: center; width: 180px;">
<p style="text-align: center;">Yes</p>
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<td style="text-align: center; width: 154px;">
<p style="text-align: center;">No</p>
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<td style="text-align: center; width: 155px;">
<p style="text-align: center;">Yes</p>
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<p style="text-align: center;">100 million cells</p>
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<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center; width: 154px;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center; width: 155px;">
<p style="text-align: center;">up to 25 g of tissue</p>
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<tr style="background-color: #fff;" valign="middle">
<td style="width: 213px;">
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center; width: 208px;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center; width: 180px;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center; width: 154px;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center; width: 155px;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
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'name' => 'TARDBP Antibody',
'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>TARDBP (TAR DNA binding protein),</strong> using a recombinant protein.</span></p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p>
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View::render() - CORE/Cake/View/View.php, line 473
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×