Diagenode

Rtf1 polyclonal antibody - Classic

カタログ番号
フォーマット
価格
C15410018
(pAb-018-050)
50 µg/28 µl
$315.00
  Bulk order

Alternative names: CDG1N, GTL7

Polyclonal antibody raised in rabbit against drosophila Rtf1 (Rtf1, Paf1/RNA polymerase II complex component, homolog), using the full length recombinant protein.

Lot001
Concentration1.8 µg/µl
Species reactivityDrosophila
TypePolyclonal
PurityProtein G purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:1,000 Fig 1, Ref 1
Immunofluorescence 1:50 Ref 1
Immunoprecipitation 12 μg/IP Ref 1
ChIP 1 - 5 μg/ChIP Ref 1
  • Validation Data

    WB

    Figure 1. Western blot analysis using the Diagenode antibody directed against dRtf1
    Whole cell extracts (WCE) from drosophila larva and drosophila adults were analysed by Western blot using the Diagenode antibody against dRtf1 (Cat. No. pAb-018-050), diluted 1:1000. Size and location of the protein are indicated.

  •  実験手法
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IP
    Immunoprecipitation Read more
    ChIP-qPCR
    定量のPCRとクロマチン免疫沈降(ChIP)が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、およ... Read more
  •  資料
    Datasheet dRtf1 pAb-018-050 DATASHEET
    Datasheet description
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Rtf1 polyclonal antibody - Classic (Diagenode Cat# C15410018 Lot# 001). Click here to copy to clipboard.

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