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NF-E2 polyclonal antibody

100 μl
  Bulk order

Polyclonal antibody raised in rabbit against NF-E2 (nuclear factor (erythroid-derived 2), 45kDa), using a recombinant protein.

Concentration1.03 μg/μl
Species reactivityHuman, mouse
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 2-5 μg/ChIP Fig 1, 2
Western Blotting 1:500 - 1:3,000 Fig 3
Immunofluorescence 1:100 - 1:1,000 Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation data

    NF-E2 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against NF-E2
    ChIP assays were performed using K562 cells, the Diagenode antibody against NF-E2 (Cat. No. C15410240) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the HBE1 and GAPDH genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    NF-E2 Antibody ChIP-seq Grade

    NF-E2 Antibody for ChIP-seq

    NF-E2 Antibody for ChIP-seq assay

    NF-E2 Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NF-E2
    ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against NF-E2 (Cat. No. C15410240). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions of chromosome 11 and 12 surrounding the HB cluster and the GAPDH genes, respectively.

    NF-E2 Antibody validated in Western BlotNF-E2 Antibody validated in Western Blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against NF-E2
    Whole cell extracts from 293 cells (30 μg, figure 3A) or mouse spleen (50 μg, figure 3B) were analysed by Western blot using the Diagenode antibody against NF-E2 (Cat. No. C15410240) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    NF-E2 Antibody validated in Immunofluorescence

    Figure 4. Immunofluorescence with the Diagenode antibody directed against NF-E2
    HepG2 cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against NF-E2 (Cat. C15410240) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain.

  • Target Description

    NF-E2 (UniProt/Swiss-Prot entry Q16621) is a component of the NF-E2 complex which is essential for the regulation of erythroid and megakaryocytic maturation and differentiation. It may play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

  •  実験手法
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-seq (ab)
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    ChIP-qPCR (ab)
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  •  資料
    Datasheet NF-E2 C15410240 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  出版物

    How to properly cite this product in your work

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