Diagenode

Mll4 polyclonal antibody

Catalog Number
Format
Price
C15310100-20
20 µl
$115.00
  Bulk order
Other format



Alternative names: ALR, MLL2, Kmt2d

Polyclonal antibody raised in rabbit against mouse Mll4 (Myeloid/lymphoid or mixed-lineage leukemia protein 4), using three KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.

LotA274-001
Concentrationnot determined
Species reactivityMouse: positive. Other species: not tested.
TypePolyclonal
PurityWhole antiserum
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:1,000 - 1:5,000 Fig 1
Western Blotting 1:500 Fig 2, (1)
  • Validation data

    ELISA

    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Mll4 (cat. No. C15310100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against Mll4 (1)
    Western blot was performed on whole cell lysates from mouse embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Mll4 (ca. No. C15310100), diluted 1:500 in PBS-Tween containing 5% skimmed milk. The location of the protein of interest (~ 600 kDa) is indicated on the right; the location of the 500 kDa MW marker is shown on the left. Cells homozygous for the targeted mll4 allele (-/-) show a complete loss of Mll4 protein, whereas the Mll4 protein was detected in flp-recombined ES cells (F/F) and heterozygously targeted ES cells (+/-).

  • Target description

    Mll4 (UniProtKB/Swiss-Prot entry O14686) is involved in the methylation of lys 4 of histone H3, thus acting as a transcriptional
    regulator
    .

  •  実験手法
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ELISA
    Enzyme-linked immunosorbent assay. Read more
  •  資料
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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    Datasheet Mll4 C15310100 DATASHEET
    Polyclonal antibody raised in rabbit against mouse Mll4 (Myeloid/lymphoid or mixed-lineage leukem...
    Download
  •  Safety sheets
    SDS C15310100 MLL4 Antibody GB en Download
    SDS C15310100 MLL4 Antibody US en Download
    SDS C15310100 MLL4 Antibody BE nl Download
    SDS C15310100 MLL4 Antibody BE fr Download
    SDS C15310100 MLL4 Antibody FR fr Download
    SDS C15310100 MLL4 Antibody ES es Download
    SDS C15310100 MLL4 Antibody DE de Download
    SDS C15310100 MLL4 Antibody JP ja Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Mll4 polyclonal antibody (Diagenode Cat# C15310100-20 Lot# A274-001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    KMT2D deficiency drives lung squamous cell carcinoma andhypersensitivity to RTK-RAS inhibition.
    Pan Yuanwang et al.
    Lung squamous cell carcinoma (LUSC) represents a major subtype of lung cancer with limited treatment options. KMT2D is one of the most frequently mutated genes in LUSC (>20\%), and yet its role in LUSC oncogenesis remains unknown. Here, we identify KMT2D as a key regulator of LUSC tumorigenesis wherein Kmt2d dele...

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