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Polyclonal antibody raised in rabbit against mouse Mll4 (Myeloid/lymphoid or mixed-lineage leukemia protein 4), using three KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Mll4 (Cat. No. CS-100-100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:15,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against Mll4 (1) Western blot was performed on whole cell lysates from mouse embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Mll4 (Cat. No. CS-100-100), diluted 1:500 in PBS-Tween containing 5% skimmed milk. The location of the protein of interest (~ 600 kDa) is indicated on the right; the location of the 500 kDa MW marker is shown on the left. Cells homozygous for the targeted mll4 allele (-/-) show a complete loss of Mll4 protein, whereas the Mll4 protein was detected in flp-recombined ES cells (F/F) and heterozygously targeted ES cells (+/-).
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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