Diagenode

Mll4 polyclonal antibody

カタログ番号
フォーマット
価格
C15310100-20
20 µl
$100.00
  Bulk order
旧フォーマット



Alternative names: ALR, MLL2, Kmt2d

Polyclonal antibody raised in rabbit against mouse Mll4 (Myeloid/lymphoid or mixed-lineage leukemia protein 4), using three KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.

LotA274-001
Concentrationnot determined
Species reactivityMouse: positive. Other species: not tested.
TypePolyclonal
PurityWhole antiserum
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:1,000 - 1:5,000 Fig 1
Western Blotting 1:500 Fig 2, (1)
  • Validation data

    ELISA

    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Mll4 (cat. No. C15310100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against Mll4 (1)
    Western blot was performed on whole cell lysates from mouse embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Mll4 (ca. No. C15310100), diluted 1:500 in PBS-Tween containing 5% skimmed milk. The location of the protein of interest (~ 600 kDa) is indicated on the right; the location of the 500 kDa MW marker is shown on the left. Cells homozygous for the targeted mll4 allele (-/-) show a complete loss of Mll4 protein, whereas the Mll4 protein was detected in flp-recombined ES cells (F/F) and heterozygously targeted ES cells (+/-).

  • Target description

    Mll4 (UniProtKB/Swiss-Prot entry O14686) is involved in the methylation of lys 4 of histone H3, thus acting as a transcriptional
    regulator
    .

  •  実験手法
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ELISA
    Enzyme-linked immunosorbent assay. Read more
  •  資料
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    Datasheet Mll4 C15310100 DATASHEET
    Polyclonal antibody raised in rabbit against mouse Mll4 (Myeloid/lymphoid or mixed-lineage leukem...
    Download
  •  Safety sheets
    Mll4 polyclonal antibody SDS GB en Download
    Mll4 polyclonal antibody SDS US en Download
    Mll4 polyclonal antibody SDS DE de Download
    Mll4 polyclonal antibody SDS JP ja Download
    Mll4 polyclonal antibody SDS BE nl Download
    Mll4 polyclonal antibody SDS BE fr Download
    Mll4 polyclonal antibody SDS FR fr Download
    Mll4 polyclonal antibody SDS ES es Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Mll4 polyclonal antibody (Diagenode Cat# C15310100-20 Lot# A274-001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

イベント

  • Virtual ChIP workshop - January 18-19, 2022 or January 25-26, 2022
    Diagenode
    Jan 18-Jan 26, 2022
  • Virtual DNA Methylation - January 18-19, 2022 or January 25-26, 2022
    Diagenode
    Jan 18-Jan 26, 2022
  • Lausanne Genomics Days 2022
    Lausanne, Switzerland
    Feb 14-Feb 15, 2022
 すべてのイベントを見る

ニュース

 すべてのニュースを見る


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics