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Polyclonal antibody raised in rabbit against mouse MBD2 (methyl-CpG binding domain protein 2), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the central part of the protein.
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse MBD2 (Cat. No. CS-098-100). By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:185,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against MBD2 (1) Western blot was performed on nuclear extracts from mouse fibroblastst (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse MBD2 (Cat. No. CS-098-100), diluted 1:500 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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