Diagenode

Lamin A/C monoclonal antibody 3A6-4C11

カタログ番号
フォーマット
価格
C15200243
50 µg
$315.00
  Bulk order



other names: LMNA, LMN1, CMT2B1, LGMD1B, CDCD1, FPLD2, Lamin, CMD1A, LMNL1, CDDC, EMD2, FPLD, HGPS, LDP1, LMNC, MADA, PRO1, FPL, IDC, LFP

Polyclonal antibody raised in rabbit against LMNA (Lamin A/C) using a recombinant protein.

Lot001
Concentration1 µg/µl
Species reactivityHuman, mouse, rat, hamster, monkey.
TypeMonoclonal
PurityProtein G purified monoclonal antibody.
HostMouse
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferTBS containing 0.1 % Na-azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western blotting 1:2,000 - 1:16,000 Fig 1, 2
IP 5 µg/IP Fig 3
IF 1:1,000 Fig 4
  • Validation data

    WB

    Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C
    Western blot was performed on protein extracts from NIH3T3 using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The antibody was used at different dilutions. The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.

    WB

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C
    Western blot was performed on protein extracts from HeLa, 293T, NIH-3T3, Rat1, BHK-21 and CV-1 cells (lanes 1-6, respectively) using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.

    IP

    Figure 3. IP using the Diagenode monoclonal antibody directed against Lamin A/C
    IP was performed on whole cell extracts (300 µg) from HeLa cells ectopically expressing Flag-tagged lamin A using 5 µg of the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The immunoprecipitated proteins were subsequently analysed by Western blot with the Lamin A/C antibody as described above. Lane 3 shows the result of the IP, the input (15 µg) is shown in lane 1, lane 2 shows the cell lysate after the IP.

    IF

    Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against Lamin A/C
    HeLa cells ectopically expressing Flag-tagged lamin A were fixed with 3.7% formaldehyde, permeabilized in 0,5% Triton X-100 and blocked in TBS containing 2% BSA. The cells were stained with the Lamin A/C antibody diluted 1:1,000 for 2 hours at RT, followed by incubation with an anti mouse secondary antibody coupled to AF596 for 1 h at RT (middle). Nuclei were counter-stained with Hoechst 33342 (left). A merge of the two stainings is shown on the right.

  •  実験手法
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  •  資料
    Lamin A/C monoclonal antibody 3A6-4C11 DATASHEET
    Polyclonal antibody raised in rabbit against LMNA (Lamin A/C) using a recombinant protein.
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  •  出版物

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