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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<li>Raised against the Cpf1 nuclease from <em>Lachnospiraceae bacterium</em></li>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
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