JMJD2b polyclonal antibody

Catalog Number
100 µl
  Bulk order

Alternative names: JHDM3b, KDM4b

Polyclonal antibody raised in rabbit against human JMJD2b (Jumonji Domain containing 2b), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the central part of the protein.

Concentrationnot determined
Species reactivityHuman
PurityWhole antiserum
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:200 Fig 1
Western blotting 1:1,000 Fig 2
Immunofluorescence 1:200 Fig 3
  • Validation Data


    Figure 1. Determination of the antibody titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human JMJD2b (Cat. No. C15310104). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:3,000.

    Western Blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against JMJD2b
    Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against JMJD2b (Cat. No. C15310104) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.


    Figure 3. Immunofluorescence using the Diagenode antibody directed against JMJD2b
    HeLa cells were stained with the Diagenode antibody against JMJD2b (Cat. No. C15310104) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the JMJD2b antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  •  実験手法
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  資料
    Datasheet JMJD2b CS-104-100 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Safety sheets
    JMJD2b Antibody SDS GB en Download
    JMJD2b Antibody SDS US en Download
    JMJD2b Antibody SDS BE nl Download
    JMJD2b Antibody SDS FR fr Download
    JMJD2b Antibody SDS BE fr Download
    JMJD2b Antibody SDS ES es Download
    JMJD2b Antibody SDS DE de Download
    JMJD2b Antibody SDS JP ja Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: JMJD2b polyclonal antibody (Diagenode Cat# C15310104 Lot# A302-004 ). Click here to copy to clipboard.

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