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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the HA protein tag </strong><br />Western blot was performed on whole cell extracts from HEK293 cells transfected with an HA-tagged expression vector using the Diagenode monoclonal antibody directed against HA (Cat. No. MAb-190-050). The antibody was used at a dilution of 1:1,000. Figure 1 shows the results for untransfected cells, used as a negative control (lane 1) and for cells transfected with a HA-tagged protein (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 2, but absent in lane 1 showing the high specificity of the HA antibody. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
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<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
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<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
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<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
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<li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span> </span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li>
<li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span> </span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong>
<p>を用いたタンパク質-DNA複合体の捕捉</p>
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<li class="large-12 columns"><strong>DNA purification (DNA精製):<span> </span></strong>クロマチン逆架橋および溶出後の精製</li>
<li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span> </span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li>
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<h3 class="text-center" style="color: #b21329;">初めての方へ</h3>
<p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p>
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'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
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<div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br />
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span> </span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li>
<li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span> </span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li>
<li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span> </span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong>
<p>を用いたタンパク質-DNA複合体の捕捉</p>
</li>
<li class="large-12 columns"><strong>DNA purification (DNA精製):<span> </span></strong>クロマチン逆架橋および溶出後の精製</li>
<li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span> </span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">初めての方へ</h3>
<p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
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</div>'
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'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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'name' => 'FBXO32 promotes microenvironment underlying epithelial-mesenchymal transition via CtBP1 during tumour metastasis and brain development',
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<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
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<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
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<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
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<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
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<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
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<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
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<div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br />
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<li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span> </span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li>
<li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span> </span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li>
<li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span> </span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong>
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<h3 class="text-center" style="color: #b21329;">初めての方へ</h3>
<p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p>
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<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
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<li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span> </span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li>
<li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span> </span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong>
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<li class="large-12 columns"><strong>DNA purification (DNA精製):<span> </span></strong>クロマチン逆架橋および溶出後の精製</li>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the HA protein tag </strong><br />Western blot was performed on whole cell extracts from HEK293 cells transfected with an HA-tagged expression vector using the Diagenode monoclonal antibody directed against HA (Cat. No. MAb-190-050). The antibody was used at a dilution of 1:1,000. Figure 1 shows the results for untransfected cells, used as a negative control (lane 1) and for cells transfected with a HA-tagged protein (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 2, but absent in lane 1 showing the high specificity of the HA antibody. </small></p>
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<th>References</th>
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<td>Fig 1</td>
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<td>IP/ChIP</td>
<td>1:150 - 1:200</td>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200190_WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the HA protein tag </strong><br />Western blot was performed on whole cell extracts from HEK293 cells transfected with an HA-tagged expression vector using the Diagenode monoclonal antibody directed against HA (Cat. No. MAb-190-050). The antibody was used at a dilution of 1:1,000. Figure 1 shows the results for untransfected cells, used as a negative control (lane 1) and for cells transfected with a HA-tagged protein (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 2, but absent in lane 1 showing the high specificity of the HA antibody. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
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<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
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<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
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<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<li>Batch-specific data is available on the website</li>
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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'description' => '<p>Activation of macrophages by inflammatory stimuli induces reprogramming of mitochondrial metabolism to support the production of pro-inflammatory cytokines and nitric oxide. Hallmarks of this metabolic rewiring are downregulation of α-ketoglutarate formation by isocitrate dehydrogenase (IDH) and accumulation of glutamine-derived succinate, which enhances the inflammatory response via the activity of succinate dehydrogenase (SDH). Here, we identify the nuclear receptor Nur77 (Nr4a1) as a key upstream transcriptional regulator of this pro-inflammatory metabolic switch in macrophages. Nur77-deficient macrophages fail to downregulate IDH expression and accumulate higher levels of succinate and other TCA cycle-derived metabolites in response to inflammatory stimulation in a glutamine-independent manner. Consequently, these macrophages produce more nitric oxide and pro-inflammatory cytokines in an SDH-dependent manner. In vivo, bone marrow Nur77 deficiency exacerbates atherosclerosis development and leads to increased circulating succinate levels. In summary, Nur77 induces an anti-inflammatory metabolic state in macrophages that protects against chronic inflammatory diseases such as atherosclerosis.</p>',
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<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
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<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
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<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
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<div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br />
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
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<ol>
<li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span> </span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li>
<li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span> </span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li>
<li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span> </span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong>
<p>を用いたタンパク質-DNA複合体の捕捉</p>
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<li class="large-12 columns"><strong>DNA purification (DNA精製):<span> </span></strong>クロマチン逆架橋および溶出後の精製</li>
<li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span> </span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li>
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<h3 class="text-center" style="color: #b21329;">初めての方へ</h3>
<p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p>
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<div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br />
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span> </span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li>
<li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span> </span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li>
<li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span> </span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong>
<p>を用いたタンパク質-DNA複合体の捕捉</p>
</li>
<li class="large-12 columns"><strong>DNA purification (DNA精製):<span> </span></strong>クロマチン逆架橋および溶出後の精製</li>
<li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span> </span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li>
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</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">初めての方へ</h3>
<p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
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$name = 'ChIP-qPCR'
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'name' => 'Antibodies you can trust',
'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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'countries' => 'FR',
'modified' => '2020-09-21 15:31:06',
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'name' => 'FBXO32 promotes microenvironment underlying epithelial-mesenchymal transition via CtBP1 during tumour metastasis and brain development',
'authors' => 'Sahu S.K. et al.',
'description' => '<p>The set of events that convert adherent epithelial cells into migratory cells are collectively known as epithelial-mesenchymal transition (EMT). EMT is involved during development, for example, in triggering neural crest migration, and in pathogenesis such as metastasis. Here we discover FBXO32, an E3 ubiquitin ligase, to be critical for hallmark gene expression and phenotypic changes underlying EMT. Interestingly, FBXO32 directly ubiquitinates CtBP1, which is required for its stability and nuclear retention. This is essential for epigenetic remodeling and transcriptional induction of CtBP1 target genes, which create a suitable microenvironment for EMT progression. FBXO32 is also amplified in metastatic cancers and its depletion in a NSG mouse xenograft model inhibits tumor growth and metastasis. In addition, FBXO32 is essential for neuronal EMT during brain development. Together, these findings establish that FBXO32 acts as an upstream regulator of EMT by governing the gene expression program underlying this process during development and disease.</p>',
'date' => '2017-11-15',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/29142217',
'doi' => '',
'modified' => '2018-01-12 17:29:21',
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the HA protein tag </strong><br />Western blot was performed on whole cell extracts from HEK293 cells transfected with an HA-tagged expression vector using the Diagenode monoclonal antibody directed against HA (Cat. No. MAb-190-050). The antibody was used at a dilution of 1:1,000. Figure 1 shows the results for untransfected cells, used as a negative control (lane 1) and for cells transfected with a HA-tagged protein (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 2, but absent in lane 1 showing the high specificity of the HA antibody. </small></p>
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<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
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<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
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<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
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<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
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<li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span> </span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li>
<li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span> </span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong>
<p>を用いたタンパク質-DNA複合体の捕捉</p>
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<li class="large-12 columns"><strong>DNA purification (DNA精製):<span> </span></strong>クロマチン逆架橋および溶出後の精製</li>
<li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span> </span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li>
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<h3 class="text-center" style="color: #b21329;">初めての方へ</h3>
<p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p>
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'meta_keywords' => 'Diagenode ChIP qPCRキットは、染色体免疫沈降後の豊富なDNAを定量化。',
'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
'meta_title' => 'クロマチン免疫沈降（ChIP）および定量PCR | Diagenode',
'modified' => '2018-01-09 16:46:56',
'created' => '2014-12-11 00:22:08',
'locale' => 'jpn'
)
$description = '<div class="row">
<div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br />
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span> </span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li>
<li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span> </span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li>
<li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span> </span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong>
<p>を用いたタンパク質-DNA複合体の捕捉</p>
</li>
<li class="large-12 columns"><strong>DNA purification (DNA精製):<span> </span></strong>クロマチン逆架橋および溶出後の精製</li>
<li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span> </span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">初めての方へ</h3>
<p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;"></div>
</div>
</div>'
$name = 'ChIP-qPCR'
$document = array(
'id' => '11',
'name' => 'Antibodies you can trust',
'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
'slug' => 'antibodies-you-can-trust-poster',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-01 20:18:31',
'created' => '2015-07-03 16:05:15',
'ProductsDocument' => array(
'id' => '2117',
'product_id' => '2815',
'document_id' => '11'
)
)
$sds = array(
'id' => '773',
'name' => 'HA tag antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/HA/SDS-C15200190-HA_tag_Antibody-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2020-09-21 15:31:06',
'created' => '2020-09-21 15:31:06',
'ProductsSafetySheet' => array(
'id' => '1409',
'product_id' => '2815',
'safety_sheet_id' => '773'
)
)
$publication = array(
'id' => '3313',
'name' => 'FBXO32 promotes microenvironment underlying epithelial-mesenchymal transition via CtBP1 during tumour metastasis and brain development',
'authors' => 'Sahu S.K. et al.',
'description' => '<p>The set of events that convert adherent epithelial cells into migratory cells are collectively known as epithelial-mesenchymal transition (EMT). EMT is involved during development, for example, in triggering neural crest migration, and in pathogenesis such as metastasis. Here we discover FBXO32, an E3 ubiquitin ligase, to be critical for hallmark gene expression and phenotypic changes underlying EMT. Interestingly, FBXO32 directly ubiquitinates CtBP1, which is required for its stability and nuclear retention. This is essential for epigenetic remodeling and transcriptional induction of CtBP1 target genes, which create a suitable microenvironment for EMT progression. FBXO32 is also amplified in metastatic cancers and its depletion in a NSG mouse xenograft model inhibits tumor growth and metastasis. In addition, FBXO32 is essential for neuronal EMT during brain development. Together, these findings establish that FBXO32 acts as an upstream regulator of EMT by governing the gene expression program underlying this process during development and disease.</p>',
'date' => '2017-11-15',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/29142217',
'doi' => '',
'modified' => '2018-01-12 17:29:21',
'created' => '2018-01-12 17:29:21',
'ProductsPublication' => array(
'id' => '2571',
'product_id' => '2815',
'publication_id' => '3313'
)
)
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
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ReflectionMethod::invokeArgs() - [internal], line ??
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Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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