Diagenode

HA tag monoclonal antibody - Classic (sample size)

カタログ番号
フォーマット
価格
C15200190-10
10 µg
$90.00
  Bulk order
ブローカー情報

Monoclonal antibody raised in mouse against the HA tag (amino acid sequence YPYDVPDYA) using a KLH-conjugated synthetic peptide. The HA antibody recognizes the influenza Hemagglutinin epitope which is extensively used as a protein tag in expression vectors. This antibody is extremely specific and allows unambiguous identification and quantitative analysis of HA tagged proteins.

Lot003
Concentration1.0 µg/µl
Species reactivitySpecific for HA-tagged fusion proteins expressed in bacteria, insect, mammalian cells
TypeMonoclonal
PurityProtein A purified
HostMouse
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
Western Blotting 1:1,000 - 1:3,000 Fig 1
IP/ChIP 1:150 - 1:200
IS 1:500 - 1:1,000

* Please note that optimal antibody amount should be determined by the end-user for each specific assay condition.

  • Validation Data

    Western Blot

    Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the HA protein tag
    Western blot was performed on whole cell extracts from HEK293 cells transfected with an HA-tagged expression vector using the Diagenode monoclonal antibody directed against HA (Cat. No. MAb-190-050). The antibody was used at a dilution of 1:1,000. Figure 1 shows the results for untransfected cells, used as a negative control (lane 1) and for cells transfected with a HA-tagged protein (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 2, but absent in lane 1 showing the high specificity of the HA antibody.

  •  実験手法
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
    ChIP-qPCR
    定量のPCRとクロマチン免疫沈降(ChIP)が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、およ... Read more
  •  資料
    Datasheet HAtag MAb-190-050 DATASHEET
    Datasheet description
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: HA tag monoclonal antibody - Classic (sample size) (Diagenode Cat# C15200190-10 Lot# 003). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Nuclear Receptor Nur77 Limits the Macrophage Inflammatory Response through Transcriptional Reprogramming of Mitochondrial Metabolism.
    Koenis DS, Medzikovic L, van Loenen PB, van Weeghel M, Huveneers S, Vos M, Evers-van Gogh IJ, Van den Bossche J, Speijer D, Kim Y, Wessels L, Zelcer N, Zwart W, Kalkhoven E, de Vries CJ
    Activation of macrophages by inflammatory stimuli induces reprogramming of mitochondrial metabolism to support the production of pro-inflammatory cytokines and nitric oxide. Hallmarks of this metabolic rewiring are downregulation of α-ketoglutarate formation by isocitrate dehydrogenase (IDH) and accumulation o...

    FBXO32 promotes microenvironment underlying epithelial-mesenchymal transition via CtBP1 during tumour metastasis and brain development
    Sahu S.K. et al.
    The set of events that convert adherent epithelial cells into migratory cells are collectively known as epithelial-mesenchymal transition (EMT). EMT is involved during development, for example, in triggering neural crest migration, and in pathogenesis such as metastasis. Here we discover FBXO32, an E3 ubiquitin liga...

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics