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Alternative names: HNF3A, HNF-3A, TCF3A, TCF-3A
Polyclonal antibody raised in rabbit against FOXA1 (forkhead box A1), using a KLH-conjugated synthetic peptide.
| Lot | 39435 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution * | References |
|---|---|---|
| ChIP/ChIP-seq | 2 μg/ChIP | Fig 1, 2 |
| Western Blotting | 1:500 - 1:3,000 | Fig 3 |
| Immunoprecipitation | 2.5 μg/IP | Fig 4 |
| Immunofluorescence | 1:100 - 1:1,000 | Fig 5 |
| Immunohistochemistry | 1:100 - 1:1,000 | Fig 6 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.
Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1
ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1
ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow.
Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1
Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1
Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract).
Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1
HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain.
Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1
Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody.
FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.
| IHC Immunohistochemistry Read more |
| IP Immunoprecipitation Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-seq (ab) Read more |
| ChIP-qPCR (ab) Read more |
| Datasheet FOXA1 C15410231 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: FOXA1 polyclonal antibody (Diagenode Cat# C15410231-100 Lot# 39435). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Signal-induced enhancer activation requires Ku70 to readtopoisomerase1-DNA covalent complexes. |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'FOXA1 polyclonal antibody - Classic', 'meta_title' => 'FOXA1 polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2316', 'antibody_id' => '347', 'name' => 'FOXA1 polyclonal antibody ', 'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p> <p>Polyclonal antibody raised in rabbit against FOXA1 (forkhead box A1), using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="FOXA1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p> </div> </div>', 'label2' => '', 'info2' => '<p>FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410231-100', 'old_catalog_number' => '', 'sf_code' => 'C15410231-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'foxa1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'FOXA1 polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'FOXA1 polyclonal antibody - Classic', 'modified' => '2021-12-23 12:04:41', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '347', 'name' => 'FOXA1 polyclonal antibody - Classic', 'description' => 'FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. 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ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410231-100', 'old_catalog_number' => '', 'sf_code' => 'C15410231-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'foxa1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'FOXA1 polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'FOXA1 polyclonal antibody - Classic', 'modified' => '2021-12-23 12:04:41', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '347', 'name' => 'FOXA1 polyclonal antibody - Classic', 'description' => 'FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.', 'clonality' => '', 'isotype' => '', 'lot' => '39435', 'concentration' => '1 μg/μl ', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution <sup>*</sup></th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq</td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2.5 μg/IP</td> <td>Fig 4</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 5</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:100 - 1:1,000</td> <td>Fig 6</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-05-12 12:10:16', 'created' => '2015-07-16 17:00:26', 'select_label' => '347 - FOXA1 polyclonal antibody - Classic (39435 - 1 μg/μl - Human - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '68', 'name' => 'C15410231', 'product_id' => '2316', 'modified' => '2016-02-18 21:41:25', 'created' => '2016-02-18 21:41:25' ) ), 'Group' => array( 'Group' => array( 'id' => '68', 'name' => 'C15410231', 'product_id' => '2316', 'modified' => '2016-02-18 21:41:25', 'created' => '2016-02-18 21:41:25' ), 'Master' => array( 'id' => '2316', 'antibody_id' => '347', 'name' => 'FOXA1 Antibody', 'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p> <p>Polyclonal antibody raised in rabbit against <strong>FOXA1 (forkhead box A1)</strong>, using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="FOXA1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410231-100', 'old_catalog_number' => '', 'sf_code' => 'C15410231-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'foxa1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'FOXA1 Antibody - ChIP-seq Grade (C15410231) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'FOXA1 (Forkhead box A1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IF, IP and IHC . Alternative names: HNF3A, HNF-3A, TCF3A, TCF-3A.', 'modified' => '2021-12-23 12:04:41', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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<![CDATA[ (function(){var qs,js,q,s,d=document,gi=d.getElementById,ce=d.createElement,gt=d.getElementsByTagName,id='typef_orm',b='https://s3-eu-west-1.amazonaws.com/share.typeform.com/';if(!gi.call(d,id)){js=ce.call(d,'script');js.id=id;js.src=b+'share.js';q=gt.call(d,'script')[0];q.parentNode.insertBefore(js,q)}id=id+'_';if(!gi.call(d,id)){qs=ce.call(d,'link');qs.rel='stylesheet';qs.id=id;qs.href=b+'share-button.css';s=gt.call(d,'head')[0];s.appendChild(qs,s)}})() // ]]></script> </p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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'ACC', 'search_order' => '00-Machine', 'price_EUR' => '24000', 'price_USD' => '27500', 'price_GBP' => '21000', 'price_JPY' => '3759600', 'price_CNY' => 'Discontinued', 'price_AUD' => '68750', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-pico-sonication-device', 'meta_title' => 'Bioruptor® Pico sonication device for RNA,Chromatin and DNA shearing for Next-Generation-Sequencing | Diagenode', 'meta_keywords' => 'Bioruptor, sonication, Next-Generation-Sequencing,DNA shearing,Protein extraction', 'meta_description' => 'An all-in-one shearing system Ideal for DNA shearing for Next-Generation-Sequencing,Chromatin shearing,RNA shearing,Protein extraction from tissues and cells and FFPE DNA extraction', 'modified' => '2023-12-20 14:21:02', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-10 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> <div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2021-07-01 10:22:38', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '630', 'name' => 'Datasheet FOXA1 C15410231', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_FOXA1_C15410231.pdf', 'slug' => 'datasheet-foxa1-c15410231', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4673', 'name' => 'Signal-induced enhancer activation requires Ku70 to readtopoisomerase1-DNA covalent complexes.', 'authors' => 'Tan Y. et al.', 'description' => '<p>Enhancer activation serves as the main mechanism regulating signal-dependent transcriptional programs, ensuring cellular plasticity, yet central questions persist regarding their mechanism of activation. Here, by successfully mapping topoisomerase I-DNA covalent complexes genome-wide, we find that most, if not all, acutely activated enhancers, including those induced by 17β-estradiol, dihydrotestosterone, tumor necrosis factor alpha and neuronal depolarization, are hotspots for topoisomerase I-DNA covalent complexes, functioning as epigenomic signatures read by the classic DNA damage sensor protein, Ku70. Ku70 in turn nucleates a heterochromatin protein 1 gamma (HP1γ)-mediator subunit Med26 complex to facilitate acute, but not chronic, transcriptional activation programs. Together, our data uncover a broad, unappreciated transcriptional code, required for most, if not all, acute signal-dependent enhancer activation events in both mitotic and postmitotic cells.</p>', 'date' => '2023-02-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36747093', 'doi' => '10.1038/s41594-022-00883-8', 'modified' => '2023-04-14 09:24:10', 'created' => '2023-02-28 12:19:11', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/jp/p/foxa1-polyclonal-antibody-classic-100-mg' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array() $pro = array( 'id' => '2315', 'antibody_id' => '347', 'name' => 'FOXA1 polyclonal antibody - Classic', 'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p> <p>Polyclonal antibody raised in rabbit against FOXA1 (forkhead box A1), using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="ChIP-seq figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="ChIP-seq figure 4" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410232-ihc.jpg" alt="Immunohistochemistry" caption="false" width="150" height="201" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '25 μl', 'catalog_number' => 'C15410231-25', 'old_catalog_number' => '', 'sf_code' => 'C15410231-000032380', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '140', 'price_GBP' => '100', 'price_JPY' => '12600', 'price_CNY' => '', 'price_AUD' => '350', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => false, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'foxa1-polyclonal-antibody-classic-25-mg', 'meta_title' => 'FOXA1 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'FOXA1 (Forkhead box A1) Polyclonal Antibody validated in IHC, IP, WB, IF, ChIP-seq and ChIP-qPCR. ', 'modified' => '2020-02-27 11:23:18', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '75', 'product_id' => '2315', 'group_id' => '68' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptor-pico-sonication-device"><img src="/img/product/shearing_technologies/bioruptor_pico.jpg" alt="Bioruptor pico next gen sequencing " class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01060010</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Picoruptor Sonicator</h6> </div> </div> </li> ' $related = array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: 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Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( 'id' => '1297', 'product_id' => '2316', 'document_id' => '38' ) ) $publication = array( 'id' => '4673', 'name' => 'Signal-induced enhancer activation requires Ku70 to readtopoisomerase1-DNA covalent complexes.', 'authors' => 'Tan Y. et al.', 'description' => '<p>Enhancer activation serves as the main mechanism regulating signal-dependent transcriptional programs, ensuring cellular plasticity, yet central questions persist regarding their mechanism of activation. Here, by successfully mapping topoisomerase I-DNA covalent complexes genome-wide, we find that most, if not all, acutely activated enhancers, including those induced by 17β-estradiol, dihydrotestosterone, tumor necrosis factor alpha and neuronal depolarization, are hotspots for topoisomerase I-DNA covalent complexes, functioning as epigenomic signatures read by the classic DNA damage sensor protein, Ku70. Ku70 in turn nucleates a heterochromatin protein 1 gamma (HP1γ)-mediator subunit Med26 complex to facilitate acute, but not chronic, transcriptional activation programs. Together, our data uncover a broad, unappreciated transcriptional code, required for most, if not all, acute signal-dependent enhancer activation events in both mitotic and postmitotic cells.</p>', 'date' => '2023-02-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36747093', 'doi' => '10.1038/s41594-022-00883-8', 'modified' => '2023-04-14 09:24:10', 'created' => '2023-02-28 12:19:11', 'ProductsPublication' => array( 'id' => '6768', 'product_id' => '2316', 'publication_id' => '4673' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/36747093" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'FOXA1 polyclonal antibody - Classic', 'meta_title' => 'FOXA1 polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2316', 'antibody_id' => '347', 'name' => 'FOXA1 polyclonal antibody ', 'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p> <p>Polyclonal antibody raised in rabbit against FOXA1 (forkhead box A1), using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="FOXA1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p> </div> </div>', 'label2' => '', 'info2' => '<p>FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410231-100', 'old_catalog_number' => '', 'sf_code' => 'C15410231-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'foxa1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'FOXA1 polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'FOXA1 polyclonal antibody - Classic', 'modified' => '2021-12-23 12:04:41', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '347', 'name' => 'FOXA1 polyclonal antibody - Classic', 'description' => 'FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.', 'clonality' => '', 'isotype' => '', 'lot' => '39435', 'concentration' => '1 μg/μl ', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution <sup>*</sup></th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq</td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2.5 μg/IP</td> <td>Fig 4</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 5</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:100 - 1:1,000</td> <td>Fig 6</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-05-12 12:10:16', 'created' => '2015-07-16 17:00:26', 'select_label' => '347 - FOXA1 polyclonal antibody - Classic (39435 - 1 μg/μl - Human - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/jp/p/foxa1-polyclonal-antibody-classic-100-mg' ) $language = 'jp' $meta_keywords = '' $meta_description = 'FOXA1 polyclonal antibody - Classic' $meta_title = 'FOXA1 polyclonal antibody - Classic' $product = array( 'Product' => array( 'id' => '2316', 'antibody_id' => '347', 'name' => 'FOXA1 polyclonal antibody ', 'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p> <p>Polyclonal antibody raised in rabbit against FOXA1 (forkhead box A1), using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="FOXA1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410231-100', 'old_catalog_number' => '', 'sf_code' => 'C15410231-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'foxa1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'FOXA1 polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'FOXA1 polyclonal antibody - Classic', 'modified' => '2021-12-23 12:04:41', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '347', 'name' => 'FOXA1 polyclonal antibody - Classic', 'description' => 'FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.', 'clonality' => '', 'isotype' => '', 'lot' => '39435', 'concentration' => '1 μg/μl ', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution <sup>*</sup></th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq</td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2.5 μg/IP</td> <td>Fig 4</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 5</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:100 - 1:1,000</td> <td>Fig 6</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-05-12 12:10:16', 'created' => '2015-07-16 17:00:26', 'select_label' => '347 - FOXA1 polyclonal antibody - Classic (39435 - 1 μg/μl - Human - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '68', 'name' => 'C15410231', 'product_id' => '2316', 'modified' => '2016-02-18 21:41:25', 'created' => '2016-02-18 21:41:25' ) ), 'Group' => array( 'Group' => array( 'id' => '68', 'name' => 'C15410231', 'product_id' => '2316', 'modified' => '2016-02-18 21:41:25', 'created' => '2016-02-18 21:41:25' ), 'Master' => array( 'id' => '2316', 'antibody_id' => '347', 'name' => 'FOXA1 Antibody', 'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p> <p>Polyclonal antibody raised in rabbit against <strong>FOXA1 (forkhead box A1)</strong>, using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="FOXA1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410231-100', 'old_catalog_number' => '', 'sf_code' => 'C15410231-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'foxa1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'FOXA1 Antibody - ChIP-seq Grade (C15410231) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'FOXA1 (Forkhead box A1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IF, IP and IHC . Alternative names: HNF3A, HNF-3A, TCF3A, TCF-3A.', 'modified' => '2021-12-23 12:04:41', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-10 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> <div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2021-07-01 10:22:38', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '630', 'name' => 'Datasheet FOXA1 C15410231', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_FOXA1_C15410231.pdf', 'slug' => 'datasheet-foxa1-c15410231', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4673', 'name' => 'Signal-induced enhancer activation requires Ku70 to readtopoisomerase1-DNA covalent complexes.', 'authors' => 'Tan Y. et al.', 'description' => '<p>Enhancer activation serves as the main mechanism regulating signal-dependent transcriptional programs, ensuring cellular plasticity, yet central questions persist regarding their mechanism of activation. Here, by successfully mapping topoisomerase I-DNA covalent complexes genome-wide, we find that most, if not all, acutely activated enhancers, including those induced by 17β-estradiol, dihydrotestosterone, tumor necrosis factor alpha and neuronal depolarization, are hotspots for topoisomerase I-DNA covalent complexes, functioning as epigenomic signatures read by the classic DNA damage sensor protein, Ku70. Ku70 in turn nucleates a heterochromatin protein 1 gamma (HP1γ)-mediator subunit Med26 complex to facilitate acute, but not chronic, transcriptional activation programs. Together, our data uncover a broad, unappreciated transcriptional code, required for most, if not all, acute signal-dependent enhancer activation events in both mitotic and postmitotic cells.</p>', 'date' => '2023-02-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36747093', 'doi' => '10.1038/s41594-022-00883-8', 'modified' => '2023-04-14 09:24:10', 'created' => '2023-02-28 12:19:11', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/jp/p/foxa1-polyclonal-antibody-classic-100-mg' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array() $pro = array( 'id' => '2315', 'antibody_id' => '347', 'name' => 'FOXA1 polyclonal antibody - Classic', 'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p> <p>Polyclonal antibody raised in rabbit against FOXA1 (forkhead box A1), using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="ChIP-seq figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="ChIP-seq figure 4" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410232-ihc.jpg" alt="Immunohistochemistry" caption="false" width="150" height="201" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '25 μl', 'catalog_number' => 'C15410231-25', 'old_catalog_number' => '', 'sf_code' => 'C15410231-000032380', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '140', 'price_GBP' => '100', 'price_JPY' => '12600', 'price_CNY' => '', 'price_AUD' => '350', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => false, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'foxa1-polyclonal-antibody-classic-25-mg', 'meta_title' => 'FOXA1 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'FOXA1 (Forkhead box A1) Polyclonal Antibody validated in IHC, IP, WB, IF, ChIP-seq and ChIP-qPCR. ', 'modified' => '2020-02-27 11:23:18', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '75', 'product_id' => '2315', 'group_id' => '68' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptor-pico-sonication-device"><img src="/img/product/shearing_technologies/bioruptor_pico.jpg" alt="Bioruptor pico next gen sequencing " class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01060010</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Picoruptor Sonicator</h6> </div> </div> </li> ' $related = array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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<![CDATA[ (function(){var qs,js,q,s,d=document,gi=d.getElementById,ce=d.createElement,gt=d.getElementsByTagName,id='typef_orm',b='https://s3-eu-west-1.amazonaws.com/share.typeform.com/';if(!gi.call(d,id)){js=ce.call(d,'script');js.id=id;js.src=b+'share.js';q=gt.call(d,'script')[0];q.parentNode.insertBefore(js,q)}id=id+'_';if(!gi.call(d,id)){qs=ce.call(d,'link');qs.rel='stylesheet';qs.id=id;qs.href=b+'share-button.css';s=gt.call(d,'head')[0];s.appendChild(qs,s)}})() // ]]></script> </p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( 'id' => '4017', 'product_id' => '2316', 'application_id' => '43' ) ) $slugs = array( (int) 0 => 'chip-qpcr-antibodies' ) $applications = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'locale' => 'jpn' ) $description = '' $name = 'ChIP-qPCR (ab)' $document = array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( 'id' => '1297', 'product_id' => '2316', 'document_id' => '38' ) ) $publication = array( 'id' => '4673', 'name' => 'Signal-induced enhancer activation requires Ku70 to readtopoisomerase1-DNA covalent complexes.', 'authors' => 'Tan Y. et al.', 'description' => '<p>Enhancer activation serves as the main mechanism regulating signal-dependent transcriptional programs, ensuring cellular plasticity, yet central questions persist regarding their mechanism of activation. Here, by successfully mapping topoisomerase I-DNA covalent complexes genome-wide, we find that most, if not all, acutely activated enhancers, including those induced by 17β-estradiol, dihydrotestosterone, tumor necrosis factor alpha and neuronal depolarization, are hotspots for topoisomerase I-DNA covalent complexes, functioning as epigenomic signatures read by the classic DNA damage sensor protein, Ku70. Ku70 in turn nucleates a heterochromatin protein 1 gamma (HP1γ)-mediator subunit Med26 complex to facilitate acute, but not chronic, transcriptional activation programs. Together, our data uncover a broad, unappreciated transcriptional code, required for most, if not all, acute signal-dependent enhancer activation events in both mitotic and postmitotic cells.</p>', 'date' => '2023-02-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36747093', 'doi' => '10.1038/s41594-022-00883-8', 'modified' => '2023-04-14 09:24:10', 'created' => '2023-02-28 12:19:11', 'ProductsPublication' => array( 'id' => '6768', 'product_id' => '2316', 'publication_id' => '4673' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/36747093" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'FOXA1 polyclonal antibody - Classic', 'meta_title' => 'FOXA1 polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2316', 'antibody_id' => '347', 'name' => 'FOXA1 polyclonal antibody ', 'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p> <p>Polyclonal antibody raised in rabbit against FOXA1 (forkhead box A1), using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="FOXA1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p> </div> </div>', 'label2' => '', 'info2' => '<p>FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410231-100', 'old_catalog_number' => '', 'sf_code' => 'C15410231-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'foxa1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'FOXA1 polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'FOXA1 polyclonal antibody - Classic', 'modified' => '2021-12-23 12:04:41', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '347', 'name' => 'FOXA1 polyclonal antibody - Classic', 'description' => 'FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.', 'clonality' => '', 'isotype' => '', 'lot' => '39435', 'concentration' => '1 μg/μl ', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution <sup>*</sup></th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq</td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2.5 μg/IP</td> <td>Fig 4</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 5</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:100 - 1:1,000</td> <td>Fig 6</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-05-12 12:10:16', 'created' => '2015-07-16 17:00:26', 'select_label' => '347 - FOXA1 polyclonal antibody - Classic (39435 - 1 μg/μl - Human - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/jp/p/foxa1-polyclonal-antibody-classic-100-mg' ) $language = 'jp' $meta_keywords = '' $meta_description = 'FOXA1 polyclonal antibody - Classic' $meta_title = 'FOXA1 polyclonal antibody - Classic' $product = array( 'Product' => array( 'id' => '2316', 'antibody_id' => '347', 'name' => 'FOXA1 polyclonal antibody ', 'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p> <p>Polyclonal antibody raised in rabbit against FOXA1 (forkhead box A1), using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="FOXA1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410231-100', 'old_catalog_number' => '', 'sf_code' => 'C15410231-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'foxa1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'FOXA1 polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'FOXA1 polyclonal antibody - Classic', 'modified' => '2021-12-23 12:04:41', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '347', 'name' => 'FOXA1 polyclonal antibody - Classic', 'description' => 'FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.', 'clonality' => '', 'isotype' => '', 'lot' => '39435', 'concentration' => '1 μg/μl ', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution <sup>*</sup></th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq</td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2.5 μg/IP</td> <td>Fig 4</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 5</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:100 - 1:1,000</td> <td>Fig 6</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-05-12 12:10:16', 'created' => '2015-07-16 17:00:26', 'select_label' => '347 - FOXA1 polyclonal antibody - Classic (39435 - 1 μg/μl - Human - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '68', 'name' => 'C15410231', 'product_id' => '2316', 'modified' => '2016-02-18 21:41:25', 'created' => '2016-02-18 21:41:25' ) ), 'Group' => array( 'Group' => array( 'id' => '68', 'name' => 'C15410231', 'product_id' => '2316', 'modified' => '2016-02-18 21:41:25', 'created' => '2016-02-18 21:41:25' ), 'Master' => array( 'id' => '2316', 'antibody_id' => '347', 'name' => 'FOXA1 Antibody', 'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p> <p>Polyclonal antibody raised in rabbit against <strong>FOXA1 (forkhead box A1)</strong>, using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="FOXA1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410231-100', 'old_catalog_number' => '', 'sf_code' => 'C15410231-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'foxa1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'FOXA1 Antibody - ChIP-seq Grade (C15410231) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'FOXA1 (Forkhead box A1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IF, IP and IHC . Alternative names: HNF3A, HNF-3A, TCF3A, TCF-3A.', 'modified' => '2021-12-23 12:04:41', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-10 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> <div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2021-07-01 10:22:38', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '630', 'name' => 'Datasheet FOXA1 C15410231', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_FOXA1_C15410231.pdf', 'slug' => 'datasheet-foxa1-c15410231', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4673', 'name' => 'Signal-induced enhancer activation requires Ku70 to readtopoisomerase1-DNA covalent complexes.', 'authors' => 'Tan Y. et al.', 'description' => '<p>Enhancer activation serves as the main mechanism regulating signal-dependent transcriptional programs, ensuring cellular plasticity, yet central questions persist regarding their mechanism of activation. Here, by successfully mapping topoisomerase I-DNA covalent complexes genome-wide, we find that most, if not all, acutely activated enhancers, including those induced by 17β-estradiol, dihydrotestosterone, tumor necrosis factor alpha and neuronal depolarization, are hotspots for topoisomerase I-DNA covalent complexes, functioning as epigenomic signatures read by the classic DNA damage sensor protein, Ku70. Ku70 in turn nucleates a heterochromatin protein 1 gamma (HP1γ)-mediator subunit Med26 complex to facilitate acute, but not chronic, transcriptional activation programs. Together, our data uncover a broad, unappreciated transcriptional code, required for most, if not all, acute signal-dependent enhancer activation events in both mitotic and postmitotic cells.</p>', 'date' => '2023-02-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36747093', 'doi' => '10.1038/s41594-022-00883-8', 'modified' => '2023-04-14 09:24:10', 'created' => '2023-02-28 12:19:11', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/jp/p/foxa1-polyclonal-antibody-classic-100-mg' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array() $pro = array( 'id' => '2315', 'antibody_id' => '347', 'name' => 'FOXA1 polyclonal antibody - Classic', 'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p> <p>Polyclonal antibody raised in rabbit against FOXA1 (forkhead box A1), using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="ChIP-seq figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="ChIP-seq figure 4" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410232-ihc.jpg" alt="Immunohistochemistry" caption="false" width="150" height="201" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '25 μl', 'catalog_number' => 'C15410231-25', 'old_catalog_number' => '', 'sf_code' => 'C15410231-000032380', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '140', 'price_GBP' => '100', 'price_JPY' => '12600', 'price_CNY' => '', 'price_AUD' => '350', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => false, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'foxa1-polyclonal-antibody-classic-25-mg', 'meta_title' => 'FOXA1 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'FOXA1 (Forkhead box A1) Polyclonal Antibody validated in IHC, IP, WB, IF, ChIP-seq and ChIP-qPCR. ', 'modified' => '2020-02-27 11:23:18', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '75', 'product_id' => '2315', 'group_id' => '68' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptor-pico-sonication-device"><img src="/img/product/shearing_technologies/bioruptor_pico.jpg" alt="Bioruptor pico next gen sequencing " class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01060010</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Picoruptor Sonicator</h6> </div> </div> </li> ' $related = array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( 'id' => '4017', 'product_id' => '2316', 'application_id' => '43' ) ) $slugs = array( (int) 0 => 'chip-qpcr-antibodies' ) $applications = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'locale' => 'jpn' ) $description = '' $name = 'ChIP-qPCR (ab)' $document = array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( 'id' => '1297', 'product_id' => '2316', 'document_id' => '38' ) ) $publication = array( 'id' => '4673', 'name' => 'Signal-induced enhancer activation requires Ku70 to readtopoisomerase1-DNA covalent complexes.', 'authors' => 'Tan Y. et al.', 'description' => '<p>Enhancer activation serves as the main mechanism regulating signal-dependent transcriptional programs, ensuring cellular plasticity, yet central questions persist regarding their mechanism of activation. Here, by successfully mapping topoisomerase I-DNA covalent complexes genome-wide, we find that most, if not all, acutely activated enhancers, including those induced by 17β-estradiol, dihydrotestosterone, tumor necrosis factor alpha and neuronal depolarization, are hotspots for topoisomerase I-DNA covalent complexes, functioning as epigenomic signatures read by the classic DNA damage sensor protein, Ku70. Ku70 in turn nucleates a heterochromatin protein 1 gamma (HP1γ)-mediator subunit Med26 complex to facilitate acute, but not chronic, transcriptional activation programs. Together, our data uncover a broad, unappreciated transcriptional code, required for most, if not all, acute signal-dependent enhancer activation events in both mitotic and postmitotic cells.</p>', 'date' => '2023-02-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36747093', 'doi' => '10.1038/s41594-022-00883-8', 'modified' => '2023-04-14 09:24:10', 'created' => '2023-02-28 12:19:11', 'ProductsPublication' => array( 'id' => '6768', 'product_id' => '2316', 'publication_id' => '4673' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/36747093" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'FOXA1 polyclonal antibody - Classic', 'meta_title' => 'FOXA1 polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2316', 'antibody_id' => '347', 'name' => 'FOXA1 polyclonal antibody ', 'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p> <p>Polyclonal antibody raised in rabbit against FOXA1 (forkhead box A1), using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="FOXA1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p> </div> </div>', 'label2' => '', 'info2' => '<p>FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410231-100', 'old_catalog_number' => '', 'sf_code' => 'C15410231-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'foxa1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'FOXA1 polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'FOXA1 polyclonal antibody - Classic', 'modified' => '2021-12-23 12:04:41', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '347', 'name' => 'FOXA1 polyclonal antibody - Classic', 'description' => 'FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.', 'clonality' => '', 'isotype' => '', 'lot' => '39435', 'concentration' => '1 μg/μl ', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution <sup>*</sup></th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq</td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2.5 μg/IP</td> <td>Fig 4</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 5</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:100 - 1:1,000</td> <td>Fig 6</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-05-12 12:10:16', 'created' => '2015-07-16 17:00:26', 'select_label' => '347 - FOXA1 polyclonal antibody - Classic (39435 - 1 μg/μl - Human - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/jp/p/foxa1-polyclonal-antibody-classic-100-mg' ) $language = 'jp' $meta_keywords = '' $meta_description = 'FOXA1 polyclonal antibody - Classic' $meta_title = 'FOXA1 polyclonal antibody - Classic' $product = array( 'Product' => array( 'id' => '2316', 'antibody_id' => '347', 'name' => 'FOXA1 polyclonal antibody ', 'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p> <p>Polyclonal antibody raised in rabbit against FOXA1 (forkhead box A1), using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="FOXA1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410231-100', 'old_catalog_number' => '', 'sf_code' => 'C15410231-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'foxa1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'FOXA1 polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'FOXA1 polyclonal antibody - Classic', 'modified' => '2021-12-23 12:04:41', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '347', 'name' => 'FOXA1 polyclonal antibody - Classic', 'description' => 'FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.', 'clonality' => '', 'isotype' => '', 'lot' => '39435', 'concentration' => '1 μg/μl ', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution <sup>*</sup></th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq</td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2.5 μg/IP</td> <td>Fig 4</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 5</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:100 - 1:1,000</td> <td>Fig 6</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-05-12 12:10:16', 'created' => '2015-07-16 17:00:26', 'select_label' => '347 - FOXA1 polyclonal antibody - Classic (39435 - 1 μg/μl - Human - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '68', 'name' => 'C15410231', 'product_id' => '2316', 'modified' => '2016-02-18 21:41:25', 'created' => '2016-02-18 21:41:25' ) ), 'Group' => array( 'Group' => array( 'id' => '68', 'name' => 'C15410231', 'product_id' => '2316', 'modified' => '2016-02-18 21:41:25', 'created' => '2016-02-18 21:41:25' ), 'Master' => array( 'id' => '2316', 'antibody_id' => '347', 'name' => 'FOXA1 Antibody', 'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p> <p>Polyclonal antibody raised in rabbit against <strong>FOXA1 (forkhead box A1)</strong>, using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="FOXA1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="FOXA1 Antibody ChIP-seq Grade" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="FOXA1 Antibody for ChIP-seq" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="FOXA1 Antibody for ChIP-seq assay" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="FOXA1 Antibody validated in ChIP-seq" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="FOXA1 Antibody validated in Western Blot" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="FOXA1 Antibody validated in Immunoprecipitation" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="FOXA1 Antibody validated in Immunofluorescence" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IHC.jpg" alt="FOXA1 Antibody validated in Immunohistochemistry" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>FOXA1 (UniProt/Swiss-Prot entry P55317) belongs to the forkhead class of transcription factors. FOXA1 was originally discovered as a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. It is also involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, as well as in cell cycle regulation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410231-100', 'old_catalog_number' => '', 'sf_code' => 'C15410231-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'foxa1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'FOXA1 Antibody - ChIP-seq Grade (C15410231) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'FOXA1 (Forkhead box A1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IF, IP and IHC . Alternative names: HNF3A, HNF-3A, TCF3A, TCF-3A.', 'modified' => '2021-12-23 12:04:41', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var qs,js,q,s,d=document,gi=d.getElementById,ce=d.createElement,gt=d.getElementsByTagName,id='typef_orm',b='https://s3-eu-west-1.amazonaws.com/share.typeform.com/';if(!gi.call(d,id)){js=ce.call(d,'script');js.id=id;js.src=b+'share.js';q=gt.call(d,'script')[0];q.parentNode.insertBefore(js,q)}id=id+'_';if(!gi.call(d,id)){qs=ce.call(d,'link');qs.rel='stylesheet';qs.id=id;qs.href=b+'share-button.css';s=gt.call(d,'head')[0];s.appendChild(qs,s)}})() // ]]></script> </p> <center><iframe width="560" height="315" src="https://www.youtube.com/embed/ckLc4owudIM" frameborder="0" allowfullscreen="allowfullscreen"></iframe></center><center> <p></p> </center><center><a href="https://www.diagenode.com/en/pages/osha"><img src="https://www.diagenode.com/img/banners/banner-osha-580.jpg" width="635" height="243" /></a></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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<![CDATA[ (function(){var qs,js,q,s,d=document,gi=d.getElementById,ce=d.createElement,gt=d.getElementsByTagName,id='typef_orm',b='https://s3-eu-west-1.amazonaws.com/share.typeform.com/';if(!gi.call(d,id)){js=ce.call(d,'script');js.id=id;js.src=b+'share.js';q=gt.call(d,'script')[0];q.parentNode.insertBefore(js,q)}id=id+'_';if(!gi.call(d,id)){qs=ce.call(d,'link');qs.rel='stylesheet';qs.id=id;qs.href=b+'share-button.css';s=gt.call(d,'head')[0];s.appendChild(qs,s)}})() // ]]></script> </p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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'ACC', 'search_order' => '00-Machine', 'price_EUR' => '24000', 'price_USD' => '27500', 'price_GBP' => '21000', 'price_JPY' => '3759600', 'price_CNY' => 'Discontinued', 'price_AUD' => '68750', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-pico-sonication-device', 'meta_title' => 'Bioruptor® Pico sonication device for RNA,Chromatin and DNA shearing for Next-Generation-Sequencing | Diagenode', 'meta_keywords' => 'Bioruptor, sonication, Next-Generation-Sequencing,DNA shearing,Protein extraction', 'meta_description' => 'An all-in-one shearing system Ideal for DNA shearing for Next-Generation-Sequencing,Chromatin shearing,RNA shearing,Protein extraction from tissues and cells and FFPE DNA extraction', 'modified' => '2023-12-20 14:21:02', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-10 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> <div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2021-07-01 10:22:38', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '630', 'name' => 'Datasheet FOXA1 C15410231', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_FOXA1_C15410231.pdf', 'slug' => 'datasheet-foxa1-c15410231', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4673', 'name' => 'Signal-induced enhancer activation requires Ku70 to readtopoisomerase1-DNA covalent complexes.', 'authors' => 'Tan Y. et al.', 'description' => '<p>Enhancer activation serves as the main mechanism regulating signal-dependent transcriptional programs, ensuring cellular plasticity, yet central questions persist regarding their mechanism of activation. Here, by successfully mapping topoisomerase I-DNA covalent complexes genome-wide, we find that most, if not all, acutely activated enhancers, including those induced by 17β-estradiol, dihydrotestosterone, tumor necrosis factor alpha and neuronal depolarization, are hotspots for topoisomerase I-DNA covalent complexes, functioning as epigenomic signatures read by the classic DNA damage sensor protein, Ku70. Ku70 in turn nucleates a heterochromatin protein 1 gamma (HP1γ)-mediator subunit Med26 complex to facilitate acute, but not chronic, transcriptional activation programs. Together, our data uncover a broad, unappreciated transcriptional code, required for most, if not all, acute signal-dependent enhancer activation events in both mitotic and postmitotic cells.</p>', 'date' => '2023-02-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36747093', 'doi' => '10.1038/s41594-022-00883-8', 'modified' => '2023-04-14 09:24:10', 'created' => '2023-02-28 12:19:11', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/jp/p/foxa1-polyclonal-antibody-classic-100-mg' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array() $pro = array( 'id' => '2315', 'antibody_id' => '347', 'name' => 'FOXA1 polyclonal antibody - Classic', 'description' => '<p>Alternative names: <strong>HNF3A</strong>, <strong>HNF-3A</strong>, <strong>TCF3A</strong>, <strong>TCF-3A</strong></p> <p>Polyclonal antibody raised in rabbit against FOXA1 (forkhead box A1), using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIP.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA1 Cat. No. C15410231) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the FBXL18, RAD51B and TFF1 genes, used as positive controls, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-A.jpg" alt="ChIP-seq figure 1" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-B.jpg" alt="ChIP-seq figure 2" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-C.jpg" alt="ChIP-seq figure 3" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-ChIPseq-D.jpg" alt="ChIP-seq figure 4" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA1</strong><br /> ChIP was performed on sheared chromatin from 4 million HepG2 cells using 2 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and an 800 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the FBXL18 and RAD51B positive control genes (fig 2C and D). The position of the amplicon used for qPCR ChIP validation is indicated by an arrow. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-WB.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against FOXA1</strong><br /> Nuclear extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IP.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against FOXA1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HepG2 cells using 2.5 μg of the Diagenode antibody against FOXA1 (Cat. No. C15410231). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated FOXA1 protein was detected by western blot with the FOXA1 antibody diluted 1:1,000. The IP with the FOXA1 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HepG2 whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410231-FOXA1-IF.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against FOXA1</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against FOXA1 (Cat. C15410231) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410232-ihc.jpg" alt="Immunohistochemistry" caption="false" width="150" height="201" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 6. Immunohistochemistry using the Diagenode antibody directed against FOXA1</strong><br /> Formalin fixed paraffin embedded cervical carcinoma (fig 6A) or breast carcinoma (fig 6B) tissue was stained with the Diagenode antibody against FOXA1 (Cat. No. C15410231) diluted 1:500 followed by a peroxidase labelled goat anti- rabbit secondary antibody. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '25 μl', 'catalog_number' => 'C15410231-25', 'old_catalog_number' => '', 'sf_code' => 'C15410231-000032380', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '140', 'price_GBP' => '100', 'price_JPY' => '12600', 'price_CNY' => '', 'price_AUD' => '350', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => false, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'foxa1-polyclonal-antibody-classic-25-mg', 'meta_title' => 'FOXA1 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'FOXA1 (Forkhead box A1) Polyclonal Antibody validated in IHC, IP, WB, IF, ChIP-seq and ChIP-qPCR. ', 'modified' => '2020-02-27 11:23:18', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '75', 'product_id' => '2315', 'group_id' => '68' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptor-pico-sonication-device"><img src="/img/product/shearing_technologies/bioruptor_pico.jpg" alt="Bioruptor pico next gen sequencing " class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01060010</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Picoruptor Sonicator</h6> </div> </div> </li> ' $related = array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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11:01:33', 'created' => '2018-03-06 11:01:33', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( 'id' => '4017', 'product_id' => '2316', 'application_id' => '43' ) ) $slugs = array( (int) 0 => 'chip-qpcr-antibodies' ) $applications = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'locale' => 'jpn' ) $description = '' $name = 'ChIP-qPCR (ab)' $document = array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( 'id' => '1297', 'product_id' => '2316', 'document_id' => '38' ) ) $publication = array( 'id' => '4673', 'name' => 'Signal-induced enhancer activation requires Ku70 to readtopoisomerase1-DNA covalent complexes.', 'authors' => 'Tan Y. et al.', 'description' => '<p>Enhancer activation serves as the main mechanism regulating signal-dependent transcriptional programs, ensuring cellular plasticity, yet central questions persist regarding their mechanism of activation. Here, by successfully mapping topoisomerase I-DNA covalent complexes genome-wide, we find that most, if not all, acutely activated enhancers, including those induced by 17β-estradiol, dihydrotestosterone, tumor necrosis factor alpha and neuronal depolarization, are hotspots for topoisomerase I-DNA covalent complexes, functioning as epigenomic signatures read by the classic DNA damage sensor protein, Ku70. Ku70 in turn nucleates a heterochromatin protein 1 gamma (HP1γ)-mediator subunit Med26 complex to facilitate acute, but not chronic, transcriptional activation programs. Together, our data uncover a broad, unappreciated transcriptional code, required for most, if not all, acute signal-dependent enhancer activation events in both mitotic and postmitotic cells.</p>', 'date' => '2023-02-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36747093', 'doi' => '10.1038/s41594-022-00883-8', 'modified' => '2023-04-14 09:24:10', 'created' => '2023-02-28 12:19:11', 'ProductsPublication' => array( 'id' => '6768', 'product_id' => '2316', 'publication_id' => '4673' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/36747093" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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