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ELAVL3 polyclonal antibody

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C15410348

100 µg

$380.00
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Polyclonal antibody raised in rabbit against human ELAVL3 (ELAV Like RNA Binding Protein 3), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.

Lot	001
Concentration	1 μg/μl
Species reactivity	Human, mouse: positive. Other species: not tested.
Type	Polyclonal
Purity	Affinity purified polyclonal antibody.
Host	Rabbit
Storage Conditions	Store at -20°C.
Storage Buffer	PBS containing 50% glycerol, does not contain a preservative.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
RIP	15 µg per 6x10⁶ cells	Fig 1
Western blotting	1:1,000	Fig 2
IP	5 µg per 2.5x10⁶ cells	Fig 3

Validation data

Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL3
Immunoprecipitation was performed on total RNA isolated from 6 million SK-N-SH cells using 15 µg of the Diagenode antibody against ELAVL3 (Cat. No. C15410348) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL3 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.

Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3
Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (Cat. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3
Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 µg of the Diagenode antibody against ELAVL3 (Cat. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.

資料
ELAVL3 polyclonal antibody datasheet DATASHEET
Polyclonal antibody raised in rabbit against human ELAVL3 (ELAV Like RNA Binding Protein 3), usin... Download

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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on total RNA isolated from 6 million SK-N-SH cells using 15 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL3 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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$related_products = ''
$rrbs_service = array(
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	(int) 3 => (int) 2684,
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	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
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$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
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	'position' => '10',
	'parent_id' => '40',
	'name' => 'IP',
	'description' => '<p>Immunoprecipitation</p>',
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	'online' => true,
	'tabular' => true,
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	'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
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	'created' => '2015-07-08 13:46:50',
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	'name' => 'IP',
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	'created' => '2015-07-08 13:46:50',
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$description = '<p>Immunoprecipitation</p>'
$name = 'IP'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
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$dataForView = array(
	'language' => 'jp',
	'meta_keywords' => '',
	'meta_description' => '',
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			'antibody_id' => '531',
			'name' => 'ELAVL3 polyclonal antibody',
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410348-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on total RNA isolated from 6 million SK-N-SH cells using 15 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL3 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<div class="row">
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410348-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410348-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.</small></p>
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<td>Fig 1</td>
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<td>Western blotting</td>
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<td>Fig 2</td>
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410348-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on total RNA isolated from 6 million SK-N-SH cells using 15 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL3 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410348-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="small-7 columns">
<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.</small></p>
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<th>References</th>
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<td>Fig 1</td>
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<td>Western blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Batch-specific data is available on the website</li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on total RNA isolated from 6 million SK-N-SH cells using 15 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL3 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.</small></p>
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<td>Fig 1</td>
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<tr>
<td>Western blotting</td>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410348-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on total RNA isolated from 6 million SK-N-SH cells using 15 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL3 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 &micro;g of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.</small></p>
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		'id' => '531',
		'name' => 'ELAVL3 polyclonal antibody',
		'description' => 'ELAVL3 (UniProtKB/Swiss-Prot entry Q14576) is a neural- specific member of the ELAVL RNA-binding protein family. It selectively binds to AU-rich sequences in several mRNA’s including VEGF mRNA. The observation that ELAVL3 is recognized by the anti-Hu antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy suggests it is involved in neuronal differentiation and maintenance.',
		'clonality' => '',
		'isotype' => '',
		'lot' => '001',
		'concentration' => '1 μg/μl',
		'reactivity' => 'Human, mouse: positive. Other species: not tested.',
		'type' => 'Polyclonal',
		'purity' => 'Affinity purified polyclonal antibody.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>RIP</td>
<td>15 &micro;g per 6x10<sup>6</sup> cells</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
</tr>
<tr>
<td>IP</td>
<td>5 &micro;g per 2.5x10<sup>6</sup> cells</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>',
		'storage_conditions' => 'Store at -20°C.',
		'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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		'modified' => '2018-07-03 17:12:45',
		'created' => '2018-06-27 15:21:12',
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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<div style="text-align: justify;" class="large-12 columns">
<p><span style="font-weight: 400;">Diagenode&rsquo;s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA-associated proteins studies below.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>
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			'meta_description' => 'Diagenode offers Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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